Glycitin

Catalog No.S3825 Synonyms: Glycitein-7-β-O-glucoside

For research use only.

Glycitin (Glycitein-7-β-O-glucoside), a natural isoflavone isolated from legumes, has antibacterial, antiviral and estrogenic activities and may exerts preventative effects on alcoholism, cardiovascular and cerebrovascular diseases and some types of cancer.

Glycitin Chemical Structure

CAS No. 40246-10-4

Purity & Quality Control

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Biological Activity

Description Glycitin (Glycitein-7-β-O-glucoside), a natural isoflavone isolated from legumes, has antibacterial, antiviral and estrogenic activities and may exerts preventative effects on alcoholism, cardiovascular and cerebrovascular diseases and some types of cancer.
In vitro

Glycitin promotes cell proliferation, osteoblast induction, and activates Col I mRNA expression and ALP activity of BMSCs. Glycitin promotes the proliferation and migration of human dermal fibroblast cells through TGF-β and p-AKT signaling[1].

In vivo Glycitin significantly prevents bone loss in variectomized (ovx) rats at a dose of 50 mg/kg/d. At this dose glycitin also prevents ovx-induced uterine atrophy and increases in body weight gain, abdominal fat, serum total cholesterol and triglyceride, and urinary excretion of pyridinoline and deoxypyridinoline with statistical significance[2].

Protocol (from reference)

Cell Research:[1]
  • Cell lines: Bone marrow stem cells (BMSCs)
  • Concentrations: 0.01, 0.5, 1, 5 and 10 µM
  • Incubation Time: 7 days
  • Method: BMSCs (1-2×106 cells or 1-2×104 per well) are cultured in 6- or 96-well culture plates overnight at 37°C in an atmosphere containing 5% CO2. Glycitin is added to the wells at final concentrations of 0.01, 0.5, 1, 5 and 10 µM and cultured for 7 days. In cells cultured in 6-well culture plates, BMSCs are determined using Oil Red O staining and observed via light microscopy at 510 nm. BMSCs are fixed using 5% precooled paraformaldehyde for 30 min at 4°C and stained with 0.6% (w/v) Oil Red O solution for 15 min at room temperature. Cells stained with Oil Red O are washed with water (3×5 min) to remove unbound dye, and culture dishes are stained with 1 ml isopropyl alcohol for 10 min. In cells cultured in 96-well culture plates, BMSCs are determined via MTT assay. A total of 20 µl MTT (5 g/l) are added to each well and cultured for 4 h. The supernatant is removed and 200 µl dimethylsulfoxide are added to each well for 15 min. Optical density (OD) is measured using a microplate spectrophotometer at 570 nm. Proliferation rate is calculated using: OD treated / OD control × 100%.
Animal Research:[2]
  • Animal Models: Female Sprague-Dawley rats
  • Dosages: 25, 50 or 100 mg/kg/d
  • Administration: oral

Solubility (25°C)

In vitro

Chemical Information

Molecular Weight 446.40
Formula

C22H22O10

CAS No. 40246-10-4
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles COC1=C(C=C2C(=C1)C(=O)C(=CO2)C3=CC=C(C=C3)O)OC4C(C(C(C(O4)CO)O)O)O

In vivo Formulation Calculator (Clear solution)

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Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Molarity Calculator

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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