Dansylcadaverine (Monodansyl cadaverine)

Catalog No.E0451 Synonyms: MDC, Monodansyl cadaverine

For research use only.

Dansylcadaverine (Monodansyl cadaverine, MDC) is an autofluorescent compound used for the labeling of autophagic vacuoles. Dansylcadaverine, a high affinity substrate of transglutaminases, can block the receptor-mediated endocytosis of many ligands.

Dansylcadaverine (Monodansyl cadaverine) Chemical Structure

CAS No. 10121-91-2

Purity & Quality Control

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Biological Activity

Description

Dansylcadaverine (Monodansyl cadaverine, MDC) is an autofluorescent compound used for the labeling of autophagic vacuoles. Dansylcadaverine, a high affinity substrate of transglutaminases, can block the receptor-mediated endocytosis of many ligands.

In vitro

Dansylcadaverine is an autofluorescent compound used for the labeling of autophagic vacuoles, which is able to serve as a substrate for transglutaminases and to block competitively the crosslinking of fibrin molecules.[3]

Protocol (from reference)

Cell Research:

[3]

  • Cell lines: MCF7 cells
  • Concentrations: 50 mM
  • Incubation Time: 15 min
  • Method:

    MCF7 cells (2.4 ×104 ) are seeded into 35 mm plates. After 24 h incubation, CuO NPs are added with an increasing concentration in the presence or absence of 3-MA for different time periods. The cells are then incubated with 50 mM monodansylcadaverine at 37℃ for 15 min and washed with 1×PBS three times with 5 min interval. Finally, the cells are observed under a fluorescence microscope.

Solubility (25°C)

In vitro

Chemical Information

Molecular Weight 335.46
Formula

C17H25N3O2S

CAS No. 10121-91-2
Storage 3 years -20°C powder
2 years -80°C in solvent

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