Coomassie Blue R-250

Synonyms: Brilliant Blue R250

Coomassie Blue R-250 (Brilliant blue R250), an anionic dye, is the most popular stain to detect proteins resolved in SDS-PAGE gels.

Coomassie Blue R-250 Chemical Structure

Coomassie Blue R-250 Chemical Structure

CAS: 6104-59-2

Purity & Quality Control

Batch: E270401 DMSO] 100 mg/mL] false] Ethanol] 3 mg/mL] false] Water] 2 mg/mL] false Purity: 99%
99

Coomassie Blue R-250 Related Products

Biological Activity

Description Coomassie Blue R-250 (Brilliant blue R250), an anionic dye, is the most popular stain to detect proteins resolved in SDS-PAGE gels.

Chemical Information & Solubility

Molecular Weight 827.98 Formula

C45H45N3O7S2.Na

CAS No. 6104-59-2 SDF --
Smiles [Na;v0].CCOC1=CC=C(NC2=CC=C(C=C2)C(C3=CC=C(C=C3)N(CC)CC4=CC(=CC=C4)[S;v6](O)(=O)=O)=C5C=CC(C=C5)=[N+;v4](CC)CC6=CC(=CC=C6)[S;v6]([O-])(=O)=O)C=C1
Storage (From the date of receipt) 3 years -20°C powder

In vitro
Batch:

DMSO : 100 mg/mL ( (120.77 mM); Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : 3 mg/mL

Water : 2 mg/mL


Molecular Weight Calculator

In vivo
Batch:

Add solvents to the product individually and in order.


In vivo Formulation Calculator

Preparing Stock Solutions

Molarity Calculator

Mass Concentration Volume Molecular Weight

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

mg/kg g μL

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO % % Tween 80 % ddH2O
%DMSO %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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