Name: BMS309403
Brand: Selleck Chemicals
SKU: S6622
Availability: InStock
Rating: 5 (8 reviews)

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Quality Control

Batch: Purity: 99.94%

99.94

Related Targets	Dehydrogenase HSP Transferase P450 (e.g. CYP17) PDE phosphatase PPAR Vitamin Carbohydrate Metabolism Mitochondrial Metabolism
Other FABP Products	FABP4 Antibody [M24L23] FABP5 Antibody [D10L18] FABP7 Antibody [B3J10]

Cell Culture, Treatment & Working Concentration

Cell Lines	Assay Type	Concentration	Incubation Time	Activity Description	PMID
BL21(DE3)	Function assay			Displacement of 1,8-ANS from His6-tagged FABP4 (unknown origin) expressed in Escherichia coli BL21(DE3) cells by fluorescence assay, Ki=0.22μM	27460668
BL21(DE3)	Function assay			Direct binding to His6-tagged FABP4 (unknown origin) expressed in Escherichia coli BL21(DE3) cells by isothermal titration calorimetry, Kd=2.05μM	27460668
aP2+/+ macrophage cells	Function assay		24 hrs	Decrease in MCP1 production in mouse aP2+/+ macrophage cells after 24 hrs	17554340
aP2-/-R macrophage cells	Function assay		24 hrs	Decrease in MCP1 production in mouse aP2-/-R macrophage cells (re-expression of aP2) after 24 hrs	17554340
THP1	Function assay	25 uM	3 days	Reduction of transformation of human THP1 cells to foam cells at 25 uM after 3 days relative to control	17554340
aP2+/+ macrophage cells	Function assay	25 uM		Reduction in total cholesterol ester content in mouse aP2+/+ macrophage cells at 25 uM relative to control	17554340
aP2-/-R macrophage cells	Function assay	25 uM		Reduction in total cholesterol ester content in mouse aP2-/-R macrophage cells (re-expression of aP2) at 25 uM relative to control	17554340
THP1	Function assay	25 uM		Increase in APOA1-mediated cholesterol in human THP1 cells at 25 uM relative to control	17554340
THP1	Function assay	25 uM		Increase in ABCA1 mRNA level in human THP1 cells at 25 uM relative to control	17554340
THP1	Function assay	25 uM		Increase in ABCA1 protein level in human THP1 cells at 25 uM relative to control	17554340
aP2+/+ macrophage cells	Function assay	25 uM		Increase in cholesterol efflux in mouse aP2+/+ macrophage cells at 25 uM relative to control	17554340
aP2-/-R macrophage cells	Function assay	25 uM		Increase in cholesterol efflux in mouse aP2-/-R macrophage cells (re-expression of aP2) at 25 uM relative to control	17554340
THP1	Function assay			Reduction of Mcp1 expression in human THP1 cells expressing aP2 relative to control	17554340
aP2+/+ macrophage cells	Function assay			Reduction of Mcp1 expression mouse aP2+/+ macrophage cells relative to control	17554340
aP2-/-R macrophage cells	Function assay			Reduction of Mcp1 expression mouse aP2-/-R macrophage cells (re-expression of aP2) relative to control	17554340
THP1	Function assay			Reduction of IL1-beta expression in human THP1 cells expressing aP2 relative to control	17554340
aP2+/+ macrophage cells	Function assay			Reduction of IL1-beta expressed in mouse aP2+/+ macrophage cells relative to control	17554340
aP2-/-R macrophage cells	Function assay			Reduction of IL1-beta expressed in mouse aP2-/-R macrophage cells (re-expression of aP2) relative to control	17554340
THP1	Function assay			Reduction of IL6 expression in human THP1 cells expressing aP2 relative to control	17554340
aP2+/+ macrophage cells	Function assay			Reduction of IL6 expression mouse aP2+/+ macrophage cells relative to control	17554340
aP2-/-R macrophage cells	Function assay			Reduction of IL6 expression mouse aP2-/-R macrophage cells (re-expression of aP2) relative to control	17554340
THP1	Function assay			Reduction of TNF expression in human THP1 cells expressing aP2 relative to control	17554340
aP2+/+ macrophage cells	Function assay			Reduction of TNF expression in mouse aP2+/+ macrophage cells relative to control	17554340
aP2-/-R macrophage cells	Function assay			Reduction of TNF expression mouse aP2-/-R macrophage cells (re-expression of aP2) relative to control	17554340
Click to View More Cell Line Experimental Data

Solubility

In vitro
Batch:

DMSO : 95 mg/mL (200.18 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)

Ethanol : 18 mg/mL

Water : Insoluble

Molarity Calculator

Mass	Concentration	Volume	Molecular Weight
Mass value Mass unit	Concentration value Concentration unit	Volume value Volume unit	Molecular weight (g/mol)

Dilution Calculator Molecular Weight Calculator

In vivo batch selection In vivo Batch:
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	6.000mg/ml (12.64mM)	Taking the 1 mL working solution as an example, add 50 μL of 120 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	4.750mg/ml (10.01mM)	Taking the 1 mL working solution as an example, add 50 μL of 95 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Clear solution	5% DMSO 1 95% Corn oil Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	0.475mg/ml (1.00mM)	Taking the 1 mL working solution as an example, add 50 μL of 9.5 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg

Average weight of animals: g

Dosing volume per animal: μL

Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO

%

% Tween 80

% ddH₂O

% DMSO

+

%

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Chemical Information, Storage & Stability

Molecular Weight	474.55	Formula	C₃₁H₂₆N₂O₃	Storage (From the date of receipt)	3 years -20°C powder
CAS No.	300657-03-8	--		Storage of Stock Solutions	1 year-80°Cin solvent 1 month-20°Cin solvent
Synonyms	N/A			Smiles	CCC1=C(C(=NN1C2=CC=CC=C2C3=CC(=CC=C3)OCC(=O)O)C4=CC=CC=C4)C5=CC=CC=C5

Mechanism of Action

Targets/IC₅₀/Ki	FABP4 (Cell-free assay) 4 nM(Kd)
In vitro	Treatment of BMS309403 (FABP4 inhibitor) comobined with SSO (CD36 inhibitor) significantly decreases the breast cancer cell viability, proliferative, migratory, and invasive abilities over 72 h. Inhibiting either at recommended concentrations does not significantly decrease the breast cancer cell viability over 72 h. Treatment with both SSO and this compound results in clustering of cells and increased lipid content, indicating an inability to metabolise lipids resulting potentially in lipotoxicity-induced apoptosis.
In vivo	Treatment with either BMS309403 or SSO alone decreases the rate of tumour formation and the combination of this compound and SSO significantly decreased the rate of tumour formation.
References	[1] https://pubmed.ncbi.nlm.nih.gov/34561446/ [2] https://pubmed.ncbi.nlm.nih.gov/21296956/

Tech Support

Handling Instructions

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BMS309403 FABP4 Inhibitor

Chemical Structure

Molecular Weight: 474.55