Biotin-HPDP

Name: Biotin-HPDP
Brand: Selleck Chemicals
SKU: E5990
Rating: 5 (1 reviews)

Catalog No.E5990 Purity:97.51%

Biotin-HPDP is a thiol-specific biotinylating reagent that forms a reversible disulfide bond with free cysteine residues on proteins or peptides. It is widely used to selectively tag and detect sulfhydryl groups, especially in biochemical assays. In the Biotin Switch assay, Biotin-HPDP labels newly exposed cysteine thiols after reduction of S-nitrosylated or S-sulfhydrated proteins, enabling detection and purification via streptavidin-based methods.

Biotin-HPDP Chemical Structure

CAS No. 129179-83-5

Purity & Quality Control

Batch: E599001 DMSO]6 mg/mL]false]Water]Insoluble]false]Ethanol]Insoluble]false Purity: 97.51%

97.51

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Biological Activity

Description

References

[1] Jaffrey SR, et al. Sci STKE. 2001 Jun 12;2001(86):pl1.

Chemical Information & Solubility

Molecular Weight	539.78	Formula	C₂₄H₃₇N₅O₃S₃
CAS No.	129179-83-5	SDF	Download Biotin-HPDP SDF
Storage (From the date of receipt)	3 years-20°Cpowder	Storage of Stock Solutions Aliquot the stock solutions to avoid repeated freeze-thaw cycles	1 year-80°Cin solvent
Storage (From the date of receipt)	3 years-20°Cpowder		1 month-20°Cin solvent

In vitro
Batch:

DMSO : 6 mg/mL ( (11.11 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : Insoluble

Ethanol : Insoluble

Molecular Weight Calculator

In vivo
Batch:

Add solvents to the product individually and in order.

In vivo Formulation Calculator

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In vivo Formulation Calculator (Clear solution)

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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
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as vortex, ultrasound or hot water bath can be used to aid dissolving.

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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