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Biocytin Dyes chemical

Cat.No.S9886

Biocytin ((+)-Biocytin, Biotinyl-L-lysine) is a conjugate of D-biotin and L-lysine. This compound is an attractive neuronal tracer. It labels neurons fast over long distances, and it can be used in conjunction with calcium dyes to report on neuronal activity in retrogradely labeled live neurons.
Biocytin Dyes chemical Chemical Structure

Chemical Structure

Molecular Weight: 372.48

Jump to Mechanism of Action Solubility Chemical Information, Storage & Stability

Quality Control

Batch: S988601 DMSO]75 mg/mL]false]Water]75 mg/mL]false]Ethanol]2 mg/mL]false Purity: 99.43%
99.43

Chemical Information, Storage & Stability

Molecular Weight 372.48 Formula
C16H28N4O4S
 Storage (From the date of receipt) 3 years -20°C powder
CAS No. 576-19-2 -- Storage of Stock Solutions

Synonyms (+)-Biocytin, Biotinyl-L-lysine Smiles NC(CCCCNC(=O)CCCCC1SCC2NC(=O)NC12)C(O)=O

Solubility

In vitro
Batch:

DMSO : 75 mg/mL (201.35 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)

Water : 75 mg/mL

Ethanol : 2 mg/mL

Molarity Calculator

Mass Concentration Volume Molecular Weight

In vivo
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In vivo Formulation Calculator (Clear solution)

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Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
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Mechanism of Action

In vitro

This compound labels type II cells via ion channels activated by [Ca2+]out reduction, probably "CALHM-like" channels, on the basolateral membrane.[3]
In vivo

This compound confers stability to the molecules and increases their applicability as neuronal tract tracer.[2]
References

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