Biocytin

For research use only.

Catalog No.S9886 Synonyms: (+)-Biocytin, Biotinyl-L-lysine

Biocytin Chemical Structure

CAS No. 576-19-2

Biocytin ((+)-Biocytin, Biotinyl-L-lysine) is a conjugate of D-biotin and L-lysine. Biocytin is an attractive neuronal tracer. It labels neurons fast over long distances, and it can be used in conjunction with calcium dyes to report on neuronal activity in retrogradely labeled live neurons.

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Biological Activity

Description Biocytin ((+)-Biocytin, Biotinyl-L-lysine) is a conjugate of D-biotin and L-lysine. Biocytin is an attractive neuronal tracer. It labels neurons fast over long distances, and it can be used in conjunction with calcium dyes to report on neuronal activity in retrogradely labeled live neurons.
In vitro

Biocytin labels type II cells via ion channels activated by [Ca2+]out reduction, probably "CALHM-like" channels, on the basolateral membrane.[3]

In vivo

Biocytin confers stability to the molecules and increases their applicability as neuronal tract tracer.[2]

Protocol

Cell Research:

[3]

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  • Cell lines: Taste receptor cells (type II cells)
  • Concentrations: 2 mg/ml
  • Incubation Time: 5 min
  • Method:

    Peeled epithelia are mounted on a recording platform and then are examined under a microscope equipped with a 60× water-immersion objective. Recording electrodes are attached to the basolateral membranes of single TBCs. In these voltage-clamp experiments, voltage-gated currents are amplified and filtered at 10 kHz using a voltage-clamp amplifier. Data are digitized using an A/D converter and are then stored using pCLAMP data acquisition and analysis software on a personal computer. The recording electrodes are filled with an intracellular CsCl solution using a pipette with a resistance of approximately 5 MΩ in physiological saline. To immunohistochemically identify cell types, biocytin (2 mg/ml) is injected through the recording electrodes.


    (Only for Reference)
Animal Research:

[2]

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  • Animal Models: Albino rats (Sprague−Dawley)
  • Dosages: --
  • Administration: ICV
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 75 mg/mL (201.35 mM)
Water 75 mg/mL (201.35 mM)
Ethanol '''2 mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 372.48
Formula
C16H28N4O4S
CAS No. 576-19-2
Storage powder
in solvent
Synonyms (+)-Biocytin, Biotinyl-L-lysine

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Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID