AS1949490

AS1949490, a competitive inhibitor of 5'-lipid phosphatase SHIP2 with IC50 values of 0.62 µM and 0.34 µM for human (Ki value is 0.44 µM) and mouse respectively, promotes protein kinase C-dependent stabilization of brain-derived neurotrophic factor mRNA in cultured cortical neurons.

AS1949490 Chemical Structure

AS1949490 Chemical Structure

CAS: 1203680-76-5

Purity & Quality Control

Batch: E040001 DMSO] 74 mg/mL] false] Ethanol] 37 mg/mL] false] Water] Insoluble] false Purity: 99.86%
99.86

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Choose Selective SHIP Inhibitors

Biological Activity

Description AS1949490, a competitive inhibitor of 5'-lipid phosphatase SHIP2 with IC50 values of 0.62 µM and 0.34 µM for human (Ki value is 0.44 µM) and mouse respectively, promotes protein kinase C-dependent stabilization of brain-derived neurotrophic factor mRNA in cultured cortical neurons.
Targets
Human SHIP2 [1] Human SHIP2 [1] Mouse SHIP2 [1]
0.44 μM(Ki) 0.62 μM 0.62 μM
In vitro
In vitro

AS1949490 increases the phosphorylation of Akt, glucose consumption and glucose uptake in L6 myotubes. In FAO hepatocytes, AS1949490 suppresses gluconeogenesis.[1]

Cell Research Cell lines L6 myotubes, FAO hepatocytes
Concentrations 1-16 µM
Incubation Time 15 min, 24 h, 48 h
Method

L6 myotubes are incubated for 15 min both with and without AS1949490 treatment before being stimulated with insulin at indicated concentration and 1 nM or fetal bovine serum at indicated concentrations for 10 min. Protein lysates from L6 myotubes are immunoblotted with anti-phosphorylated Akt antibody or anti-Akt antibody. FAO cells are treated with AS19494940 and insulin for 24 h. The glucose concentration in the medium is measured after incubation for 6 h in gluconeogenesis buffer.

In Vivo
In vivo

Acute administration of AS1949490 inhibites the expression of gluconeogenic genes in the livers of normal mice. Chronic treatment of diabetic db/db mice with AS1949490 significantly loared the plasma glucose level and improved glucose intolerance.[1]

Chemical Information & Solubility

Molecular Weight 371.88 Formula

C20H18ClNO2S

CAS No. 1203680-76-5 SDF --
Smiles CC(NC(=O)C1=C(OCC2=CC=C(Cl)C=C2)C=CS1)C3=CC=CC=C3
Storage (From the date of receipt) 3 years -20°C powder

In vitro
Batch:

DMSO : 74 mg/mL ( (198.98 mM); Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : 37 mg/mL

Water : Insoluble


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In vivo
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Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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