5-Fluoroorotic acid

For research use only.

Catalog No.S9873 Synonyms: 5-FOA, 5-Fluoroorotate, NSC 31712

5-Fluoroorotic acid Chemical Structure

CAS No. 703-95-7

5-Fluoroorotic acid (5-FOA, 5-Fluoroorotate, NSC 31712) is a selective agent in yeast molecular genetics. 5-Fluoroorotic acid possesses a well-expressed anticandidal effect close to that of 5-fluorocytosine, as well as moderate antidermatophytal effects.

Purity & Quality Control

Biological Activity

Description 5-Fluoroorotic acid (5-FOA, 5-Fluoroorotate, NSC 31712) is a selective agent in yeast molecular genetics. 5-Fluoroorotic acid possesses a well-expressed anticandidal effect close to that of 5-fluorocytosine, as well as moderate antidermatophytal effects.
In vitro

5-FOA is an extremely useful reagent for the selection of Ura- cells amid a population of Ura+ cells. The selection is effective in transformation and recombination studies where loss of URA3+ is desired. A new plasmid shuffling procedure based on the 5-FOAR selection permits the recovery of conditional lethal mutations in cloned genes that encode vital functions.[1]

Protocol

Cell Research:

[1]

- Collapse
  • Cell lines: Yeast cells
  • Concentrations: 1 mg/ml, 500 μg/ml
  • Incubation Time: 4-7 days
  • Method:

    Yeast cells are generally pregrown in liquid YPD medium or on YPD plates prior to selection on 5-FOA. As many as 107 cells may be plated on a single, 50 mm Petri dish containing 5-FOA medium. Resistant colonies will grow up within 4-7 days at 30°. The most useful concentration of the drug is generally 1 mg/ml 5-fluoroorotic acid, but in the interest of economy it is possible to use as little as 500 μg/ml 5-FOA. When lower concentrations of 5-FOA are used, however, background growth of the Ura+ cells is considerably greater. This background growth is less of a problem when Ura- derivatives occur at a high frequency (e.g., when a URA3+ marker is located between the elements of a direct duplication). In some instances it may be desirable to use a rich medium such as YPD as opposed to the minimal medmm normally used with 5-FOA selection.


    (Only for Reference)

Solubility (25°C)

In vitro DMSO 35 mg/mL (201.04 mM)
Water Insoluble
Ethanol Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 174.09
Formula

C5H3FN2O4

CAS No. 703-95-7
Storage powder
in solvent
Synonyms 5-FOA, 5-Fluoroorotate, NSC 31712

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
Dosage mg/kg Average weight of animals g Dosing volume per animal ul Number of animals
Step 2: Enter the in vivo formulation ()
% DMSO % % Tween 80 % ddH2O
CalculateReset

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (mg) = Concentration (mM) × Volume (mL) × Molecular Weight (g/mol)

  • Mass
    Concentration
    Volume
    Molecular Weight

*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and SDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1
    V1
    C2
    V2

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and SDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

  • * Indicates a Required Field

Related Others Products

Tags: buy 5-Fluoroorotic acid | 5-Fluoroorotic acid supplier | purchase 5-Fluoroorotic acid | 5-Fluoroorotic acid cost | 5-Fluoroorotic acid manufacturer | order 5-Fluoroorotic acid | 5-Fluoroorotic acid distributor
×
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID