research use only

3,3'-Diaminobenzidine tetrahydrochloride Dyes chemical

Name: 3,3'-Diaminobenzidine tetrahydrochloride
Brand: Selleck Chemicals
SKU: E2690
Availability: InStock
Rating: 5 (1 reviews)

Cat.No.E2690

3,3'-Diaminobenzidine tetrahydrochloride (DAB) is the most sensitive and commonly used substrate for horseradish peroxidase, which can visualize the color of cells or tissues bound by horseradish peroxidase during immunohistochemistry or in situ hybridization.

Chemical Structure

Molecular Weight: 378.1254

Jump to Mechanism of Action Solubility Chemical Information, Storage & Stability

Quality Control

Batch: E269001 DMSO]54 mg/mL]false]Water]54 mg/mL]false]Ethanol]Insoluble]false Purity: 99.51%

99.51

Related Targets	Others Antineoplastic and Immunosuppressive Antibiotics ADC Cytotoxin Selection Antibiotics for Transfected Cell PROTAC Antibiotics for Mammalian Cell Culture ADC Linker Antibiotics for Plant Cell Culture PROTAC Linker Hydrotropic Agents Antibody-Drug Conjugate E3 ligase Ligand Target Protein Ligand Molecular Glues Enzyme Substrate Drug-Linker Conjugates for ADC Chelating Agent
Related Products	CFSE Propidium Iodide D-Luciferin D-Luciferin sodium salt Crystal Violet Hematoxylin DFO WST-8 Thiazolyl Blue Dihydroethidium Hoechst 33342 JC-1 Rhodamine B DAPI Dihydrochloride Nile Red Ponceau S MKT-077

Chemical Information, Storage & Stability

Molecular Weight	378.1254	Formula	C₁₂H₁₄N₄.4ClH.H₂O	Storage (From the date of receipt)	3 years -20°C powder
CAS No.	868272-85-9	--		Storage of Stock Solutions	1 year-80°Cin solvent 1 month-20°Cin solvent
Synonyms	DAB			Smiles	O.Cl.Cl.Cl.Cl.NC1=C(N)C=C(C=C1)C2=CC(=C(N)C=C2)N

Solubility

In vitro
Batch:

DMSO : 54 mg/mL ( (142.8 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : 54 mg/mL

Ethanol : Insoluble

Molarity Calculator

Mass	Concentration	Volume	Molecular Weight
=	×	×

Dilution Calculator Molecular Weight Calculator

In vivo Batch:
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg Average weight of animals: g Dosing volume per animal:μL Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO + % + % Tween 80 + % ddH₂O

%DMSO+ %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Mechanism of Action

References	https://pubmed.ncbi.nlm.nih.gov/35715545/

Clinical Trial Information

(data from https://clinicaltrials.gov, updated on 2024-05-22)

NCT Number	Recruitment	Conditions	Sponsor/Collaborators	Start Date	Phases
NCT05974592	Recruiting	Diabetic Foot	Hacettepe University	September 1 2023	Not Applicable
NCT06251934	Completed	BRAF-positive Metastatic Melanoma	Novartis Pharmaceuticals\|Novartis	September 28 2022	--
NCT05848219	Terminated	Metastatic Melanoma	Novartis Pharmaceuticals\|Novartis	October 16 2021	--
NCT05806268	Completed	BRAF v600 Mutated Metastatic Melanoma	Novartis Pharmaceuticals\|Novartis	July 30 2021	--
NCT04092725	Completed	Pharmacokinetics	Scynexis Inc.\|Clinical Network Services (CNS) Pty Ltd	September 9 2019	Phase 1

Tech Support

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

C1=C0/X	C1:	LOG(C1):
C2=C1/X	C2:	LOG(C2):
C3=C2/X	C3:	LOG(C3):
C4=C3/X	C4:	LOG(C4):
C5=C4/X	C5:	LOG(C5):
C6=C5/X	C6:	LOG(C6):
C7=C6/X	C7:	LOG(C7):
C8=C7/X	C8:	LOG(C8):