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2,2-Diphenyl-1-picrylhydrazyl

Name: 2,2-Diphenyl-1-picrylhydrazyl
Brand: Selleck Chemicals
SKU: E2925
Availability: InStock
Rating: 5 (1 reviews)

Cat.No.E2925

2,2-Diphenyl-1-picrylhydrazyl (DPPH) is a stable free radical that can be used to measure the radical scavenging activity of antioxidants. The odd electron of nitrogen atom in DPPH is reduced by receiving a hydrogen atom from antioxidants to the corresponding hydrazine.

Chemical Structure

Molecular Weight: 394.32

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Quality Control

Batch: E292501 DMSO]79 mg/mL]false]Water]Insoluble]false]Ethanol]Insoluble]false Purity: 99.05%

Cited in Nature Medicine for its top-tier quality
COA
HPLC
SDS
Datasheet

99.05

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Solubility

In vitro
Batch:

DMSO : 79 mg/mL (200.34 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)

Water : Insoluble

Ethanol : Insoluble

Molarity Calculator

Mass	Concentration	Volume	Molecular Weight
Mass value Mass unit	Concentration value Concentration unit	Volume value Volume unit	Molecular weight (g/mol)

Dilution Calculator Molecular Weight Calculator

In vivo batch selection In vivo Batch:
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg

Average weight of animals: g

Dosing volume per animal: μL

Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO

% Tween 80

% ddH₂O

% DMSO

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Chemical Information, Storage & Stability

Molecular Weight	394.32	Formula	C₁₈H₁₂N₅O₆	Storage (From the date of receipt)	3 years -20°C powder
CAS No.	1898-66-4	--		Storage of Stock Solutions	1 year-80°Cin solvent 1 month-20°Cin solvent

Mechanism of Action

References	[1] https://pubmed.ncbi.nlm.nih.gov/23572765/

Clinical Trial Information

(data from https://clinicaltrials.gov, updated on 2024-05-22)

NCT Number	Recruitment	Conditions	Sponsor/Collaborators	Start Date	Phases
NCT03935022	Completed	Healthy	University of Milan	March 6 2019	Not Applicable
NCT05140629	Completed	Hyperglycemia Postprandial	Egas Moniz - Cooperativa de Ensino Superior CRL	January 2 2017	Not Applicable
NCT01290458	Completed	Muscle Damage\|Oxidative Stress\|Hemolysis	University of Thessaly\|European University Cyprus\|Greek Ministry of Development\|Democritus University of Thrace	June 2009	Not Applicable

Tech Support

Handling Instructions

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