ZO-3 Antibody [A9A20]

Catalog No.: F1319

For research use only.

    Home Primary Antibodies ZO-3 Antibody [A9A20]

    Application: Reactivity:

    Experiment Essentials

    WB
    Recommended SDS-PAGE separating gel concentration: 5%.

    Usage Information

    Dilution
    1:1000
    1:1600
    Application
    WB, IF
    Reactivity
    Human
    Source
    Rabbit Monoclonal Antibody
    Storage Buffer
    PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3
    Storage (from the date of receipt)
    -20°C (avoid freeze-thaw cycles), 2 years
    Predicted MW
    140 kDa
    Positive Control MCF-7 cell; HT-29 cell; HCT-15 cell
    Negative Control

    Datasheet & SDS

    Biological Description

    Specificity
    ZO-3 Antibody [A9A20] detects endogenous levels of total ZO-3 protein.
    Subcellular Location
    Cell junction, Cell membrane, Membrane, Nucleus, Tight junction
    Uniprot ID
    O95049
    Clone
    A9A20
    Synonym(s)
    Tight junction protein ZO-3; Tight junction protein 3; Zona occludens protein 3; Zonula occludens protein 3; TJP3; ZO3
    Background
    ZO-3, also known as TJP3, is a scaffolding protein of the membrane-associated guanylate kinase-like (MAGUK) family that localizes to tight junctions (TJs), where it tethers integral membrane proteins such as claudins and occludin to the actin cytoskeleton and links the conserved Crumbs polarity complex to TJs. Structurally, ZO-3 contains multiple PDZ domains, an SH3 domain, and a guanylate kinase-like domain, enabling multiprotein complex assembly at cell–cell junctions. It is expressed in epithelial and endothelial tissues, as well as in the enveloping cell layer of zebrafish embryos, consistent with its role in maintaining barrier properties. Functionally, ZO-3 is essential for the ultrastructural integrity of tight junctions, epidermal barrier function, and osmoregulation, with loss leading to edema, impaired circulation, and tissue malformations. Thus, ZO-3 acts as a key adaptor in tight junction organization, particularly critical for barrier function during early development.
    References
    • https://pubmed.ncbi.nlm.nih.gov/18275946/

    Tech Support

    Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

    Handling Instructions

    Tel: +1-832-582-8158 Ext:3
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