Datasheet

Biological Description

Specificity	ZO-3 Antibody [A9A20] detects endogenous levels of total ZO-3 protein.
Background	ZO-3, also known as TJP3, is a scaffolding protein of the membrane-associated guanylate kinase-like (MAGUK) family that localizes to tight junctions (TJs), where it tethers integral membrane proteins such as claudins and occludin to the actin cytoskeleton and links the conserved Crumbs polarity complex to TJs. Structurally, ZO-3 contains multiple PDZ domains, an SH3 domain, and a guanylate kinase-like domain, enabling multiprotein complex assembly at cell–cell junctions. It is expressed in epithelial and endothelial tissues, as well as in the enveloping cell layer of zebrafish embryos, consistent with its role in maintaining barrier properties. Functionally, ZO-3 is essential for the ultrastructural integrity of tight junctions, epidermal barrier function, and osmoregulation, with loss leading to edema, impaired circulation, and tissue malformations. Thus, ZO-3 acts as a key adaptor in tight junction organization, particularly critical for barrier function during early development.

Specificity

ZO-3 Antibody [A9A20] detects endogenous levels of total ZO-3 protein.

Background

ZO-3, also known as TJP3, is a scaffolding protein of the membrane-associated guanylate kinase-like (MAGUK) family that localizes to tight junctions (TJs), where it tethers integral membrane proteins such as claudins and occludin to the actin cytoskeleton and links the conserved Crumbs polarity complex to TJs. Structurally, ZO-3 contains multiple PDZ domains, an SH3 domain, and a guanylate kinase-like domain, enabling multiprotein complex assembly at cell–cell junctions. It is expressed in epithelial and endothelial tissues, as well as in the enveloping cell layer of zebrafish embryos, consistent with its role in maintaining barrier properties. Functionally, ZO-3 is essential for the ultrastructural integrity of tight junctions, epidermal barrier function, and osmoregulation, with loss leading to edema, impaired circulation, and tissue malformations. Thus, ZO-3 acts as a key adaptor in tight junction organization, particularly critical for barrier function during early development.

Usage Information

Application

WB, IF

Dilution

WB	IF
1:1000	1:1600

Reactivity

Human

Source

Rabbit Monoclonal Antibody

MW

140 kDa

Storage Buffer

PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage
(from the date of receipt)

-20°C (avoid freeze-thaw cycles), 2 years

Exprimental Methods

References

https://pubmed.ncbi.nlm.nih.gov/18275946/

ZO-3 Antibody [A9A20]

Biological Description

Usage Information

References

Application Data