CARD15/NOD2 Antibody [J24G22]

Catalog No.: F2966

For research use only.

    Home Primary Antibodies CARD15/NOD2 Antibody [J24G22]

    Application: Reactivity:

    Usage Information

    Dilution
    1:1000
    1:200
    Application
    WB, IHC
    Reactivity
    Human
    Source
    Mouse Monoclonal Antibody
    Storage Buffer
    PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3
    Storage (from the date of receipt)
    -20°C (avoid freeze-thaw cycles), 2 years
    Predicted MW
    115 kDa

    Datasheet & SDS

    Biological Description

    Specificity
    CARD15/NOD2 Antibody [J24G22] detects endogenous levels of total CARD15/NOD2 protein.
    Clone
    J24G22
    Synonym(s)
    CARD15; NOD2; Nucleotide-binding oligomerization domain-containing protein 2; Caspase recruitment domain-containing protein 15; Inflammatory bowel disease protein 1
    Background
    CARD15/NOD2 (nucleotide-binding and oligomerization domain 2), also known as NLRC2, is a member of the NOD-like receptor (NLR) family encoded by the CARD15 gene on chromosome 16q12–21. Structurally, NOD2 contains two N-terminal caspase recruitment domains (CARDs) that mediate protein–protein interactions, a central nucleotide-binding domain (NBD/NACHT) responsible for ATP-dependent oligomerization, and C-terminal leucine-rich repeats (LRRs) that recognize bacterial muramyl dipeptide (MDP), a conserved motif of peptidoglycan. NOD2 is primarily expressed in myeloid lineage cells, including monocytes, macrophages, and dendritic cells, but is also found in intestinal epithelial cells where it plays a critical role in mucosal immunity. Functionally, NOD2 serves as an intracellular pattern recognition receptor (PRR) that detects MDP and triggers oligomerization, leading to recruitment of RIP2 kinase via CARD–CARD interactions and subsequent activation of NF-κB and MAPK signaling pathways. This initiates proinflammatory cytokine production, antimicrobial responses, and bacterial clearance. Beyond its immune stimulatory role, NOD2 also exerts immune regulatory functions, maintaining tolerance and preventing excessive inflammation. Mutations in NOD2, such as R702W, G908R, and 1007fs, impair ligand recognition or oligomerization, resulting in defective downstream signaling and are strongly linked to Crohn’s disease and other chronic inflammatory disorders. Thus, NOD2 acts as a key molecular sensor and regulator of innate immunity, with dysregulation contributing to both inflammatory bowel disease and potentially atherosclerosis.
    References
    • https://pubmed.ncbi.nlm.nih.gov/26557013/

    Tech Support

    Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

    Handling Instructions

    Tel: +1-832-582-8158 Ext:3
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