Datasheet

Biological Description

Specificity	CARD15/NOD2 Antibody [J24G22] detects endogenous levels of total CARD15/NOD2 protein.
Background	CARD15/NOD2 (nucleotide-binding and oligomerization domain 2), also known as NLRC2, is a member of the NOD-like receptor (NLR) family encoded by the CARD15 gene on chromosome 16q12–21. Structurally, NOD2 contains two N-terminal caspase recruitment domains (CARDs) that mediate protein–protein interactions, a central nucleotide-binding domain (NBD/NACHT) responsible for ATP-dependent oligomerization, and C-terminal leucine-rich repeats (LRRs) that recognize bacterial muramyl dipeptide (MDP), a conserved motif of peptidoglycan. NOD2 is primarily expressed in myeloid lineage cells, including monocytes, macrophages, and dendritic cells, but is also found in intestinal epithelial cells where it plays a critical role in mucosal immunity. Functionally, NOD2 serves as an intracellular pattern recognition receptor (PRR) that detects MDP and triggers oligomerization, leading to recruitment of RIP2 kinase via CARD–CARD interactions and subsequent activation of NF-κB and MAPK signaling pathways. This initiates proinflammatory cytokine production, antimicrobial responses, and bacterial clearance. Beyond its immune stimulatory role, NOD2 also exerts immune regulatory functions, maintaining tolerance and preventing excessive inflammation. Mutations in NOD2, such as R702W, G908R, and 1007fs, impair ligand recognition or oligomerization, resulting in defective downstream signaling and are strongly linked to Crohn’s disease and other chronic inflammatory disorders. Thus, NOD2 acts as a key molecular sensor and regulator of innate immunity, with dysregulation contributing to both inflammatory bowel disease and potentially atherosclerosis.

Specificity

CARD15/NOD2 Antibody [J24G22] detects endogenous levels of total CARD15/NOD2 protein.

Background

CARD15/NOD2 (nucleotide-binding and oligomerization domain 2), also known as NLRC2, is a member of the NOD-like receptor (NLR) family encoded by the CARD15 gene on chromosome 16q12–21. Structurally, NOD2 contains two N-terminal caspase recruitment domains (CARDs) that mediate protein–protein interactions, a central nucleotide-binding domain (NBD/NACHT) responsible for ATP-dependent oligomerization, and C-terminal leucine-rich repeats (LRRs) that recognize bacterial muramyl dipeptide (MDP), a conserved motif of peptidoglycan. NOD2 is primarily expressed in myeloid lineage cells, including monocytes, macrophages, and dendritic cells, but is also found in intestinal epithelial cells where it plays a critical role in mucosal immunity. Functionally, NOD2 serves as an intracellular pattern recognition receptor (PRR) that detects MDP and triggers oligomerization, leading to recruitment of RIP2 kinase via CARD–CARD interactions and subsequent activation of NF-κB and MAPK signaling pathways. This initiates proinflammatory cytokine production, antimicrobial responses, and bacterial clearance. Beyond its immune stimulatory role, NOD2 also exerts immune regulatory functions, maintaining tolerance and preventing excessive inflammation. Mutations in NOD2, such as R702W, G908R, and 1007fs, impair ligand recognition or oligomerization, resulting in defective downstream signaling and are strongly linked to Crohn’s disease and other chronic inflammatory disorders. Thus, NOD2 acts as a key molecular sensor and regulator of innate immunity, with dysregulation contributing to both inflammatory bowel disease and potentially atherosclerosis.

Usage Information

Application

WB, IHC

Dilution

WB	IHC
1:1000	1:200

Reactivity

Human

Source

Mouse Monoclonal Antibody

MW

115 kDa

Storage Buffer

PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage
(from the date of receipt)

-20°C (avoid freeze-thaw cycles), 2 years

Exprimental Methods

References

https://pubmed.ncbi.nlm.nih.gov/26557013/

CARD15/NOD2 Antibody [J24G22]

Biological Description

Usage Information

References

Application Data