Sodium Monofluorophosphate

Catalog No.S4013

Monofluorophosphate is a competitive inhibitor of pyruvate kinase and alkaline phosphatase with Ki of 3.4 mM and 69 μM, respectively, which also irreversibly inhibits phosphorylase phosphatase with Ki of 0.5 mM.

Price Stock Quantity  
USD 97 In stock
Bulk Inquiry

Massive Discount Available

Free Overnight Delivery on all orders over $ 500.

Sodium Monofluorophosphate   Chemical Structure

Sodium Monofluorophosphate Chemical Structure
Molecular Weight: 143.95

Validation & Quality Control

Product Information

  • Compare Others
    Compare Others

Product Description

Biological Activity

Description Monofluorophosphate is a competitive inhibitor of pyruvate kinase and alkaline phosphatase with Ki of 3.4 mM and 69 μM, respectively, which also irreversibly inhibits phosphorylase phosphatase with Ki of 0.5 mM.
Targets pyruvate kinase alkaline phosphatase phosphorylase phosphatase
IC50 3.4 mM (Ki) 69 μM (Ki) 0.5 mM (Ki) [1]
In vitro Monofluorophosphate (1 mM) induces a three-fold increase in proliferation rate of embryonic chick calvarial cells in serum-free monolayer cultures. 30 μM of Monofluorophosphate increases the activity of DNA and alkaline phosphatase in calvarial cells by 135% and 178%, respectively. In cultured intact calvaria, 30 μM of Monofluorophosphate increases DNA activity, alkaline phosphatase activity and collagen production by 143%, 186% and 151%, respectively. [2]
In vivo Monofluorophosphate displays potent activity on bone immobilization in vivo. Rats supplied with drinking water containing 50 ppm F as Monofluorophosphate show a significant increase in the bone sickness of mobile tibia and immobile tibia, compared with fluoride-free water group (1.48 vs 0.98 mm, 0.79 vs 0.40 mm, respectively). Monofluorophosphate does not change the specific gravities of the immobilized and non-immobilized tibiae of the rats. Administration of Monofluorophosphate increases the mineralization rate of bones and counteracts the decrease in bone thickness, ash content and specific gravity induced by immobilization, without changing the Ca/P ratio of the ash. [3]
Features

Protocol(Only for Reference)

Kinase Assay: [2]

Alkaline phosphatase activity assay ALP activity is measured colorimetrically using PNPP as a substrate in carbonate/tris buffers containing 1 mM MgCl2, in 96-place tissue culture microwell plates (total reaction volume = 0.3 mL). Reactions are initiated by addition of the enzyme, and incubated at ambient room temperature (22 ℃). Production of p-nitrophenolate is monitored by measuring the time-dependent change in absorbance at 410 nm, using a Dynatech automatic microwell plate reader.
1

References

Clinical Trial Information( data from http://clinicaltrials.gov)

NCT Number Recruitment Conditions Sponsor
/Collaborators
Start Date Phases
NCT01657877 Recruiting Caries GlaxoSmithKline 2012-05 Phase 2
NCT01691560 Completed Dentine Hypersensitivity GlaxoSmithKline|BioSci Research 2012-08 Phase 2
NCT01831817 Completed Dentinal Hypersensitivity GlaxoSmithKline 2013-01 Phase 2
NCT01827670 Completed Dentine Hypersensitivity GlaxoSmithKline 2013-02 Phase 3
NCT01778933 Not yet recruiting Renal Cell Carcinoma University of Pennsylvania 2013-05 Phase 0

Chemical Information

Download Sodium Monofluorophosphate SDF
Molecular Weight (MW) 143.95
Formula

FNa2O3P

CAS No. 10163-15-2
Storage 3 years -20℃Powder
6 months-80℃in DMSO
Syonnyms Sodium Fluorophosphate
Solubility (25°C) * In vitro DMSO <1 mg/mL (<1 mM)
Water 29 mg/mL (201 mM)
Ethanol <1 mg/mL (<1 mM)
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
Chemical Name Phosphorofluoridic acid, disodium salt (8CI,9CI)

Preparing Stock Solutions

Stock Solution (1ml DMSO) 1mM 10mM 20mM 30mM
Mass(mg) 0.14395 1.4395 2.879 4.3185

Research Area

Tech Support & FAQs

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

* Indicates a Required Field

Related Others

  • SRPIN340

    SRPIN340 is a selective SRPK inhibitor with Ki of 0.89 μM for SRPK1, showing no significant inhibitory activity against more than 140 other kinases..

    Features:The only specific SRPK1 and SRPK2 inhibitor. Potential use in congenital diseases and viral diseases including HIV, HCV and HBV.

  • AZ191

    AZ191 is a potent and selective DYRK1B inhibitor with IC50 of 17 nM, about 5- and 110-fold selectivity over DYRK1A and DYRK2, respectively.

  • RI-1

    RI-1 is a RAD51 inhibitor with IC50 ranging from 5 to 30 μM.

    Features:A selective recombinant RAD51 protein inhibitor discovered in 2012. Valuable tool for mechanistic studies of DNA repair and potential for use in many cancers.

  • CX-4945 (Silmitasertib)

    CX-4945 is a potent and selective inhibitor of CK2 (casein kinase 2) with IC50 of 1 nM, less potent to Flt3, Pim1 and CDK1 (inactive in cell-based assay). Phase 1.

    Features:First clinical inhibitor of CK2.

  • Tacrolimus (FK506)

    FK-506 is a 23-membered macrolide lactone, it reduces peptidyl-prolyl isomerase activity by binding to the immunophilin FKBP12 (FK506 binding protein) creating a new complex.

  • Bendamustine HCl

    Bendamustine HCL is a DNA-damaging agent with IC50 of 50 μM.

  • Posaconazole

    Posaconazole is a sterol C14ɑ demethylase inhibitor with an IC50 of 0.25 nM.

    Features:Currently the most advanced candidate for the treatment of Chagas disease.

  • Cidofovir

    Cidofovir suppresses virus replication by selective inhibition of viral DNA synthesis.

  • Simvastatin

    Simvastatin is a competitive inhibitor of HMG-CoA reductase with Ki of 0.1-0.2 nM .

  • Streptozotocin (STZ)

    Streptozotocin is a glucosamine-nitrosourea derivative, which is a methylating, carcinogenic, antibiotic and diabetes inducing agent.

Recently Viewed Items

Tags: buy Sodium Monofluorophosphate | Sodium Monofluorophosphate supplier | purchase Sodium Monofluorophosphate | Sodium Monofluorophosphate cost | Sodium Monofluorophosphate manufacturer | order Sodium Monofluorophosphate | Sodium Monofluorophosphate distributor
Contact Us