Sodium Monofluorophosphate

Catalog No.S4013

Monofluorophosphate is a competitive inhibitor of pyruvate kinase and alkaline phosphatase with Ki of 3.4 mM and 69 μM, respectively, which also irreversibly inhibits phosphorylase phosphatase with Ki of 0.5 mM.

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Sodium Monofluorophosphate   Chemical Structure

Sodium Monofluorophosphate Chemical Structure
Molecular Weight: 143.95

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Product Information

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Product Description

Biological Activity

Description Monofluorophosphate is a competitive inhibitor of pyruvate kinase and alkaline phosphatase with Ki of 3.4 mM and 69 μM, respectively, which also irreversibly inhibits phosphorylase phosphatase with Ki of 0.5 mM.
Targets pyruvate kinase alkaline phosphatase phosphorylase phosphatase
IC50 3.4 mM (Ki) 69 μM (Ki) 0.5 mM (Ki) [1]
In vitro Monofluorophosphate (1 mM) induces a three-fold increase in proliferation rate of embryonic chick calvarial cells in serum-free monolayer cultures. 30 μM of Monofluorophosphate increases the activity of DNA and alkaline phosphatase in calvarial cells by 135% and 178%, respectively. In cultured intact calvaria, 30 μM of Monofluorophosphate increases DNA activity, alkaline phosphatase activity and collagen production by 143%, 186% and 151%, respectively. [2]
In vivo Monofluorophosphate displays potent activity on bone immobilization in vivo. Rats supplied with drinking water containing 50 ppm F as Monofluorophosphate show a significant increase in the bone sickness of mobile tibia and immobile tibia, compared with fluoride-free water group (1.48 vs 0.98 mm, 0.79 vs 0.40 mm, respectively). Monofluorophosphate does not change the specific gravities of the immobilized and non-immobilized tibiae of the rats. Administration of Monofluorophosphate increases the mineralization rate of bones and counteracts the decrease in bone thickness, ash content and specific gravity induced by immobilization, without changing the Ca/P ratio of the ash. [3]
Features

Protocol(Only for Reference)

Kinase Assay: [2]

Alkaline phosphatase activity assay ALP activity is measured colorimetrically using PNPP as a substrate in carbonate/tris buffers containing 1 mM MgCl2, in 96-place tissue culture microwell plates (total reaction volume = 0.3 mL). Reactions are initiated by addition of the enzyme, and incubated at ambient room temperature (22 ℃). Production of p-nitrophenolate is monitored by measuring the time-dependent change in absorbance at 410 nm, using a Dynatech automatic microwell plate reader.
1

References

Clinical Trial Information( data from http://clinicaltrials.gov)

NCT Number Recruitment Conditions Sponsor
/Collaborators
Start Date Phases
NCT01657877 Recruiting Caries GlaxoSmithKline 2012-05 Phase 2
NCT01691560 Completed Dentine Hypersensitivity GlaxoSmithKline|BioSci Research 2012-08 Phase 2
NCT01831817 Completed Dentinal Hypersensitivity GlaxoSmithKline 2013-01 Phase 2
NCT01827670 Completed Dentine Hypersensitivity GlaxoSmithKline 2013-02 Phase 3
NCT01778933 Not yet recruiting Renal Cell Carcinoma University of Pennsylvania 2013-05 Phase 0

Chemical Information

Download Sodium Monofluorophosphate SDF
Molecular Weight (MW) 143.95
Formula

FNa2O3P

CAS No. 10163-15-2
Storage 3 years -20℃Powder
6 months-80℃in DMSO
Syonnyms Sodium Fluorophosphate
Solubility (25°C) * In vitro DMSO <1 mg/mL (<1 <1 mM)
Water 29 mg/mL (201 mM)
Ethanol <1 mg/mL (<1 mM)
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
Chemical Name Phosphorofluoridic acid, disodium salt (8CI,9CI)

Preparing Stock Solutions

Stock Solution (1ml DMSO) 1mM 10mM 20mM 30mM
Mass(mg) 0.14395 1.4395 2.879 4.3185

Research Area

Tech Support & FAQs

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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