Sodium Monofluorophosphate

Catalog No.S4013

Sodium Monofluorophosphate is a competitive inhibitor of pyruvate kinase and alkaline phosphatase with Ki of 3.4 mM and 69 μM, respectively, which also irreversibly inhibits phosphorylase phosphatase with Ki of 0.5 mM.

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Sodium Monofluorophosphate   Chemical Structure

Sodium Monofluorophosphate Chemical Structure
Molecular Weight: 143.95

Validation & Quality Control

MSDS

Product Information

Product Description

Biological Activity

Description Sodium Monofluorophosphate is a competitive inhibitor of pyruvate kinase and alkaline phosphatase with Ki of 3.4 mM and 69 μM, respectively, which also irreversibly inhibits phosphorylase phosphatase with Ki of 0.5 mM.
Targets alkaline phosphatase [1] phosphorylase phosphatase [1] pyruvate kinase [1]
IC50 69 μM(Ki) 0.5 mM(Ki) 3.4 mM(Ki)
In vitro Monofluorophosphate (1 mM) induces a three-fold increase in proliferation rate of embryonic chick calvarial cells in serum-free monolayer cultures. 30 μM of Monofluorophosphate increases the activity of DNA and alkaline phosphatase in calvarial cells by 135% and 178%, respectively. In cultured intact calvaria, 30 μM of Monofluorophosphate increases DNA activity, alkaline phosphatase activity and collagen production by 143%, 186% and 151%, respectively. [2]
In vivo Monofluorophosphate displays potent activity on bone immobilization in vivo. Rats supplied with drinking water containing 50 ppm F as Monofluorophosphate show a significant increase in the bone sickness of mobile tibia and immobile tibia, compared with fluoride-free water group (1.48 vs 0.98 mm, 0.79 vs 0.40 mm, respectively). Monofluorophosphate does not change the specific gravities of the immobilized and non-immobilized tibiae of the rats. Administration of Monofluorophosphate increases the mineralization rate of bones and counteracts the decrease in bone thickness, ash content and specific gravity induced by immobilization, without changing the Ca/P ratio of the ash. [3]
Features

Protocol(Only for Reference)

Kinase Assay: [2]

Alkaline phosphatase activity assay ALP activity is measured colorimetrically using PNPP as a substrate in carbonate/tris buffers containing 1 mM MgCl2, in 96-place tissue culture microwell plates (total reaction volume = 0.3 mL). Reactions are initiated by addition of the enzyme, and incubated at ambient room temperature (22 ℃). Production of p-nitrophenolate is monitored by measuring the time-dependent change in absorbance at 410 nm, using a Dynatech automatic microwell plate reader.

Conversion of different model animals based on BSA (Value based on data from FDA Draft Guidelines)

SpeciesMouseRatRabbitGuinea pigHamsterDog
Weight (kg)0.020.151.80.40.0810
Body Surface Area (m2)0.0070.0250.150.050.020.5
Km factor36128520
Animal A (mg/kg) = Animal B (mg/kg) multiplied by  Animal B Km
Animal A Km

For example, to modify the dose of resveratrol used for a mouse (22.4 mg/kg) to a dose based on the BSA for a rat, multiply 22.4 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for resveratrol of 11.2 mg/kg.

Rat dose (mg/kg) = mouse dose (22.4 mg/kg) ×  mouse Km(3)  = 11.2 mg/kg
rat Km(6)
1

References

[1] Pearce EI. Caries Res, 1983, Suppl 1, 21-35.

[2] Farley JR, et al. Calcif Tissue Int, 1987, 40(1), 35-42.

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Clinical Trial Information( data from http://clinicaltrials.gov, updated on 2015-08-29)

NCT Number Recruitment Conditions Sponsor
/Collaborators
Start Date Phases
NCT02371616 Completed Dentine Sensitivity GlaxoSmithKline September 2014 --
NCT01827670 Completed Dentine Hypersensitivity GlaxoSmithKline February 2013 Phase 3
NCT01831817 Completed Dentinal Hypersensitivity GlaxoSmithKline January 2013 Phase 2
NCT01691560 Completed Dentine Hypersensitivity GlaxoSmithKline|BioSci Research August 2012 Phase 2
NCT01592851 Completed Dentine Hypersensitivity GlaxoSmithKline April 2012 Phase 3

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Chemical Information

Download Sodium Monofluorophosphate SDF
Molecular Weight (MW) 143.95
Formula

FNa2O3P

CAS No. 10163-15-2
Storage 3 years -20℃powder
6 months-80℃in solvent
Synonyms NSC248
Solubility (25°C) * In vitro Water 29 mg/mL (201.45 mM)
DMSO <1 mg/mL (<1 mM)
Ethanol <1 mg/mL (<1 mM)
In vivo
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
Chemical Name Phosphorofluoridic acid, disodium salt (8CI,9CI)

Tech Support & FAQs

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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