Home Immunology & Inflammation phosphatase inhibitor - ROS activator Sanguinarine chloride For research use only.

Sanguinarine chloride

Sanguinarine, a plant alkaloid, is a potent and specific protein phosphatase (PP) 2C inhibitor. Sanguinarine chloride can stimulate apoptosis via activating the production of reactive oxygen species (ROS). Sanguinarine-induced apoptosis is associated with the activation of JNK and NF-κB.

Sanguinarine chloride Chemical Structure

Sanguinarine chloride Chemical Structure

CAS: 5578-73-4

Selleck's Sanguinarine chloride has been cited by 2 publications

Purity & Quality Control

Batch: S545201 DMSO] 5 mg/mL] false] Water] Insoluble] false] Ethanol] Insoluble] false Purity: 99.63%
99.63

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Biological Activity

Description Sanguinarine, a plant alkaloid, is a potent and specific protein phosphatase (PP) 2C inhibitor. Sanguinarine chloride can stimulate apoptosis via activating the production of reactive oxygen species (ROS). Sanguinarine-induced apoptosis is associated with the activation of JNK and NF-κB.
Targets
PP2C [1]
(Cell-free)
0.68 μM(Ki)
In vitro
In vitro Sanguinarine inhibited PP2C competitively with respect to α-casein (Ki=0.68 μM) and showed selectivity for PP2C as compared with PP1, PP2A, and PP2B in vitro. In vivo, sanguinarine showed cytotoxicity toward human promyelocytic leukemia cell line HL60, with an IC50 value of 0.37 μM, and induced apoptosis through a caspase-3/7-dependent mechanism involving the phosphorylation of p38, a PP2C substrate[1].
Cell Research Cell lines HL60 cells
Concentrations 1 μM
Incubation Time 0-6 h
Method

HL60 cells (2×105 cells/ml) were treated with 1 μM sanguinarine for the indicated durations. Thereafter, cell lysates were assayed for caspase-3/7 activity using a chemiluminescent assay. kit.

Chemical Information & Solubility

Molecular Weight 367.78 Formula

C20H14NO4.Cl
CAS No. 5578-73-4 SDF --
Smiles C[N+]1=C2C(=C3C=CC4=C(C3=C1)OCO4)C=CC5=CC6=C(C=C52)OCO6.[Cl-]
Storage (From the date of receipt) 3 years -20°C powder

In vitro
Batch:

DMSO : 5 mg/mL ( (13.59 mM)； Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : Insoluble

Ethanol : Insoluble


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In vivo
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Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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