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JX06 PDK inhibitor

Name: JX06
Brand: Selleck Chemicals
SKU: S0983
Availability: InStock
Rating: 5 (1 reviews)

Cat.No.S0983

JX06 is a selective covalent inhibitor of PDK1 in cells. This compound dose-dependently inhibits PDK1, PDK2 and PDK3 with IC50 0.049 μM, 0.101 μM and 0.313 μM, respectively.

Chemical Structure

Molecular Weight: 324.51

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Quality Control

Batch: S098301 DMSO]65 mg/mL]false]Water]Insoluble]false]Ethanol]Insoluble]false Purity: 99.99%

Cited in Nature Medicine for its top-tier quality
COA
NMR
SDS
Datasheet

99.99

Related Targets	PI3K Akt mTOR GSK-3 ATM/ATR DNA-PK AMPK PDPK1 PTEN PP2A
Other PDK Inhibitors	BX-795 BX-912

Cell Culture, Treatment & Working Concentration

Cell Lines	Assay Type	Concentration	Incubation Time	Activity Description	PMID
Kelly	Antiproliferative assay		72 hrs	Antiproliferative activity against human Kelly cells after 72 hrs by CCK8 assay, IC50=0.289μM.	28230989
A549	Antiproliferative assay		72 hrs	Antiproliferative activity against human A549 cells after 72 hrs by CCK8 assay, IC50=0.48μM.	28230989
A549	Antitumor activity assay	40 to 80 mg/kg	21 days	Antitumor activity against human A549 cells xenografted in BALB/c nude mouse assessed as reduction in tumor weight at 40 to 80 mg/kg, ip for 21 days measured on day 21 post dose	28230989
Click to View More Cell Line Experimental Data

Solubility

In vitro
Batch:

DMSO : 65 mg/mL (200.3 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)

Water : Insoluble

Ethanol : Insoluble

Molarity Calculator

Mass	Concentration	Volume	Molecular Weight
Mass value Mass unit	Concentration value Concentration unit	Volume value Volume unit	Molecular weight (g/mol)

Dilution Calculator Molecular Weight Calculator

In vivo batch selection In vivo Batch:
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution	5% DMSO 1 40% PEG 300 2 5%Tween80 3 50%saline Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	2.500mg/ml (7.70mM)	Taking the 1 mL working solution as an example, add 50 μL of mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of saline to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg

Average weight of animals: g

Dosing volume per animal: μL

Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO

% Tween 80

% ddH₂O

% DMSO

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Chemical Information, Storage & Stability

Molecular Weight	324.51	Formula	C₁₀H₁₆N₂O₂S₄	Storage (From the date of receipt)	3 years -20°C powder
CAS No.	729-46-4	--		Storage of Stock Solutions	1 year-80°Cin solvent 1 month-20°Cin solvent
Synonyms	N/A			Smiles	C1COCCN1C(=S)SSC(=S)N2CCOCC2

Mechanism of Action

Targets/IC₅₀/Ki	PDK1 (Cell-free assay) 0.049 μM PDK2 (Cell-free assay) 0.101 μM PDK3 (Cell-free assay) 0.313 μM
References	[1] https://pubmed.ncbi.nlm.nih.gov/26483203/

Tech Support

Handling Instructions

Tel: +1-832-582-8158 Ext:3

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