Home Endocrinology & Hormones Estrogen/progestogen Receptor agonist Equol For research use only.

Equol

Equol ,a metabolite of soybeans, is an important isoflavone in humans，specifically binds to 5α-DHT, has a modest affinity for recombinant estrogen receptor ERβ. Phase 2.

Equol Chemical Structure

Equol Chemical Structure

CAS: 531-95-3

Selleck's Equol has been cited by 1 publication

Purity & Quality Control

Batch: Purity: 99.07%
99.07

Equol Related Products

Signaling Pathway

Estrogen/progestogen Receptor Signaling Pathways

Estrogen/progestogen Receptor Signaling Pathway

Choose Selective Estrogen/progestogen Receptor Inhibitors

Biological Activity

Description Equol ,a metabolite of soybeans, is an important isoflavone in humans，specifically binds to 5α-DHT, has a modest affinity for recombinant estrogen receptor ERβ. Phase 2.
Features A chiral molecule that can exist as the enantiomers R-equol and S-equol.
Targets
ERβ [1]
In vitro
In vitro Equol is a metabolite produced from the soy phytoestrogen daidzein by the action of gut microflora. Equol has higher affinity for both ERs (estrogen receptors including ERalpha and ERbeta). Equol exists as the enantiomers R-equol and S-equol. S-equol has a high binding affinity, preferential for ERbeta with a Ki of 16 nM whereas R-equol binds more weakly and with a preference for ERalpha K with a Ki of 50 nM. [1] Equol is superior to all other isoflavones in its antioxidant activity. [2] Equol has antioestrogenic properties. [3] Equol is a 100-fold more potent than daidzein in stimulating an oestrogenic response. Equol is also more effective than daidzein in competing with 3 H-oestradiol for binding to the ER. Equol stimulates the growth of MCF-7 cells in a concentration-dependent manner. Although equol exhibits oestrogenic activity, exposure of MCF-7 cells to equol simultaneously with oestradiol is effective in reducing pS2 mRNA expression. Equol results in the downregulation of ER mRNA expression. [4]
Cell Research Cell lines MCF-7 cells
Concentrations 0 μM-10 μM
Incubation Time 24 hours
Method Each well of a 24-well plate is seeded with 1 × 105 MCF-7 cells in 1 mL of media B. Twenty-four hours after plating, equol at the indicated concentration is added. Equol is dissolved in ethanol (final concentration of ethanol in the medium is 1%). The medium is changed every 24 hours and equol is replenished with each change. Cell growth is determined on the sixth day by the sulphorhodamine colorimetric assay. After colour development, aliquots are pipetted into a 96-well microtitre plate and the absorbance is determined at 570 nm using an Elisa microplate reader.
NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT06133101 Not yet recruiting
Non-Alcoholic Fatty Liver Disease
Marialena Mouzaki|Children''s Hospital Medical Center Cincinnati
 November 2023 Phase 2
NCT01982578 Completed
Alzheimer''s Disease
Fundación para la Investigación del Hospital Clínico de Valencia|University of Valencia
 September 1 2017 Not Applicable
NCT03101085 Completed
Alzheimer Disease
Russell Swerdlow|Ausio Pharmaceuticals LLC|University of Kansas Medical Center
 May 5 2017 Phase 1|Phase 2
NCT02142777 Completed
Alzheimer''s Disease
Russell Swerdlow MD|Ausio Pharmaceuticals LLC|University of Kansas Medical Center
 July 2014 Phase 1

Chemical Information & Solubility

Molecular Weight 242.27 Formula

C15H14O3
CAS No. 531-95-3 SDF Download Equol SDF
Smiles C1C(COC2=C1C=CC(=C2)O)C3=CC=C(C=C3)O
Storage (From the date of receipt)

In vitro
Batch:

DMSO : 48 mg/mL ( (198.12 mM)； Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : 48 mg/mL

Water : Insoluble


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In vivo
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