Home Immunology & Inflammation Immunology & Inflammation related chemical Echinocystic acid For research use only.

Echinocystic acid

Echinocystic acid (EA), a natural triterpone enriched in various herbs, displays a range of pharmacological activities including anti-inflammatory and antioxidant effects.

Echinocystic acid Chemical Structure

Echinocystic acid Chemical Structure

CAS: 510-30-5

Purity & Quality Control

Batch: S383701 DMSO] 94 mg/mL] false] ] ] false] ] ] false Purity: 98%
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Biological Activity

Description Echinocystic acid (EA), a natural triterpone enriched in various herbs, displays a range of pharmacological activities including anti-inflammatory and antioxidant effects.
In vitro
In vitro Echinocystic acid (EA) could induce apoptosis in human HepG2 cells, as characterized by DNA fragmentation, activation of caspase-3, -8, and -9, and PARP cleavage. EA induces the truncation of Bid protein and reduction of Bcl-2 protein. EA also causes the loss of mitochondrial membrane potential (DWm) and cytochrome c release from mitochondria to cytosol. Moreover, EA could activate c-Jun NH2-terminal kinase (JNK) and p38 kinase, and JNK-specific inhibitor SP600125 and p38 kinase-specific inhibitor SB200235 could block serial molecular events of EA-induced apoptosis such as Bid truncation, Bcl-2 reduction, cytochrome c release, caspase activation, and DNA fragmentation in HepG2 cells. EA inhibits HepG2 cell proliferation in a dose-dependent manner with an IC50 value of 45.4 μM at 24h treatment[1].
Cell Research Cell lines HepG2 cells
Concentrations 0, 15, 30, 45, 60, 100 μM
Incubation Time 24h
Method The cell survival rate is measured by a MTT assay. HepG2 cells (2 ×104/well) are incubated with different concentrations of EA in 96-well plates for 24h. MTT solution (5 mg/ml) is then added to each well and incubated for another 4h. The resulting MTT-formazan product is dissolved by the addition of 0.04N HCl-isopropanol solutions. Absorbance is measured at 595 nm with a microplate reader. The cell viability is expressed as the optical density ratio of the treatment to control.
In Vivo
In vivo Administration of Echinocystic acid (EA) is found to improve the maximum stress and Young’s modulus of femur in OVX rats. Micro-computed tomography analysis reveals that EA could improve the trabecular architecture, as shown by increasing the BV/TV, Tb.N, and Tb.Th in OVX rats. However, EA does not affect the body weight and uterine weight. EA has been shown to display an anti-inflammatory effect in different models of chronic inflammation in mice through the down-regulation of pro-inflammatory cytokines such as IL-1β, IL-18, TNF-α[2]. EA attenuates reserpine-induced pain/depression dyad partially through regulating the biogenic amines levels and GluN2B receptors in the hippocampus[3].
Animal Research Animal Models female ovariectomy (OVX) Sprague–Dawley rats
Dosages 1, 5 or 15 mg/kg/day
Administration ig

Chemical Information & Solubility

Molecular Weight 472.70 Formula

C30H48O4
CAS No. 510-30-5 SDF Download Echinocystic acid SDF
Smiles CC1(CCC2(C(C1)C3=CCC4C5(CCC(C(C5CCC4(C3(CC2O)C)C)(C)C)O)C)C(=O)O)C
Storage (From the date of receipt)

In vitro
Batch:

DMSO : 94 mg/mL ( (198.85 mM)； Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)


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In vivo
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