Home Microbiology Selection Antibiotics for Transfected Cell inhibitor Bacitracin

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Bacitracin Selection Antibiotics for Transfected Cell inhibitor

Cat.No.S4146

Bacitracin is a mixture of related cyclic polypeptides produced by organisms of the licheniformis group of Bacillus subtilis var Tracy, which disrupts both gram positive and gram negative bacteria by interfering with cell wall and peptidoglycan synthesis.
Bacitracin Selection Antibiotics for Transfected Cell inhibitor Chemical Structure

Chemical Structure

Molecular Weight: 1408.67

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  • Antibiotic Titer > 65 IU/mg
  • Cited in Nature Medicine for its top-tier quality
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Chemical Information, Storage & Stability

Molecular Weight 1408.67 Formula

C65H101N17O16S

 Storage (From the date of receipt)
CAS No. 1405-87-4 Download SDF Storage of Stock Solutions

Synonyms N/A Smiles CCC(C)C1C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NCCCCC(C(=O)NC(C(=O)N1)CCCN)NC(=O)C(C(C)CC)NC(=O)C(CCC(=O)O)NC(=O)C(CC(C)C)NC(=O)C2CSC(=N2)C(C(C)CC)N)CC(=O)N)CC(=O)O)CC3=CN=CN3)CC4=CC=CC=C4

Mechanism of Action

In vitro
Bacitracin is a potent antibiotic against gram-positive cocci, staphlococci, streptococci, corynebacteria, Treponema pallidum, T. vincenti, Actinomyces israeli, anaerobic cocci, clostridia, neisseria, most gonococci and meingococci, but it is relatively ineffective against most other gram-negative bacteria. Archaebacteria is also sensitive to this compound. Of this group, Methanobacterium arbophilicum, M. M.O.H., M. O.H.G. and M. formicium showed inhibition by this antibiotic while Methanospirilum and Methanosarcina are insensitive to it. This compound affects a number of biochemical processes in bacteria, including metal ion transport, peptidoglycan synthesis, membrane permeability, and biosynthesis of inducible enzymes. Many of the biological properties of this chemical can be attributed to its high affinity for polyisoprenyl pyrophosphates. Aside from the antibiotic activity of the Bacitracins, these peptides have proven useful as a tool for investigating lipid-protein interactions at the molecular level. It is also a known inhibitor of insulin degradation by both isolated cells and subcellular organelles, which inhibits the ability of purified glutathione-insulin transhydrogenase to split insulin into its constituent A and B chains. At concentrations of 90 and 300μM, this compound inhibits 50 and 90%, respectively, of the degrading activity of the purified enzyme. Similarly, degradation by crude liver lysates is inhibited 50 and 90% by 70 and 250 μM of this chemical, respectively. It decreases both the Vmax and affinity of the enzyme for insulin. This agent is able to inhibit the reductive function of the plasma membrane by against protein disulfide-isomerase. It is able to induce inhibition of the cleavage of a disulfide-linked iodotyramine/poly(D-lysine) conjugate which bounds nonspecifically to the surface of CHO cells. It also inhibits the cytotoxicity of diphtheria toxin, adisulfide-linked heterodimer that binds to a specific surface receptor and must undergo chain separation to exert its cytotoxicity.
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