Home Microbiology Antibiotics for Mammalian Cell Culture inhibitor Nystatin

research use only

Nystatin Antibiotics for Mammalian Cell Culture inhibitor

Cat.No.S1934

Nystatin(Fungicidin), which belongs to the polyene group of antimycotics, is frequently used as a topical agent in the treatment of oro-pharyngeal candidosis.
Nystatin Antibiotics for Mammalian Cell Culture inhibitor Chemical Structure

Chemical Structure

Molecular Weight: 926.09

Jump to

Quality Control

  • Antibiotic Titer ≥ 4400 u/mg
  • Cited in Nature Medicine for its top-tier quality
  • COA
  • Datasheet
  • SDS/MSDS

Molarity Calculator

Mass Concentration Volume Molecular Weight
Dilution Calculator Molecular Weight Calculator

In vivo
Batch:

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

mg/kg
g
μL

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO
%
% Tween 80
% ddH2O
% DMSO
+
%

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Chemical Information, Storage & Stability

Molecular Weight 926.09 Formula

C47H75NO17

 Storage (From the date of receipt)
CAS No. 1400-61-9 Download SDF Storage of Stock Solutions

Synonyms Fungicidin SMILES CC1C=CC=CCCC=CC=CC=CC=CC(CC2C(C(CC(O2)(CC(C(CCC(CC(CC(CC(=O)OC(C(C1OC3CC(C(C(O3)C)O)O)C)C)O)O)O)O)O)O)O)C(=O)O)OC4C(C(C(C(O4)C)O)N)O

Read more about storage stability stock solution CAS number SMILES

Mechanism of Action

In vitro

Nystatin results in a significant reduction in buccal epithelial cell adhesion of all six Candida species. This compound is an antibiotic that increases the permeability of plasma membranes to small monovalent ions, including chloridion. It increases apical chloridion permeability to the point where transepithelial chloridion transport is limited by transport across the basolateral membrane of tracheal epithelial cells, which reflects primarily the activity of the cotransporter. This chemical (400 units/ml) increases the basal level of transepithelial 36Cl flux approximately 1.5-fold and eliminates UTP stimulation of this flux. This treatment also abolishes UTP stimulation of saturable, basolateral [3H]bumetanide binding, a measure of functioning Na-K-Cl cotransporters in these cells; isoproterenol stimulation of binding is only mildly inhibited by nystatin treatment. It significantly enhances endostatin uptake by endothelial cells through switching endostatin internalization predominantly to the clathrin-mediated pathway. This compound-enhanced internalization of endostatin also increases its inhibitory effects on endothelial cell tube formation and migration.

In vivo

Nystatin combined with endostatin selectively enhances endostatin uptake and biodistribution in tumor blood vessels and tumor tissues but not in normal tissues of tumor-bearing mice, ultimately resulting in elevated antiangiogenic and antitumor efficacies of endostatin in vivo. Liposomal this compound, at doses as low as 2 mg/kg of body weight/day, protects neutropenic mice against Aspergillus-induced death in a statistically significant manner at the 50-day time point compared to either the no-treatment, the saline, or the empty-liposome group.

References
  • [4] https://pubmed.ncbi.nlm.nih.gov/9333054/

Clinical Trial Information

(data from https://clinicaltrials.gov, updated on 2026-09-03)

NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT03178149 RECRUITING
Age-Related Macular Degeneration
Astellas Institute for Regenerative Medicine
 2018-07-13 PHASE1
NCT06616168 ACTIVE_NOT_RECRUITING
Bacterial Vaginosis
Danylo Halytsky Lviv National Medical University
 2023-09-01 PHASE1
NCT04550546 COMPLETED
Dental Caries
University of Rochester
 2021-05-15 PHASE1
NCT03862079 WITHDRAWN
Graft-versus-host Disease Prevention
M.D. Anderson Cancer Center
 2020-06-01 PHASE2
NCT04837664 UNKNOWN
Candida Albicans Infection; Cigarette Smoking
King Saud University
 2018-07-03 PHASE3
NCT03237533 UNKNOWN
Oral Lichen Planus
University of Faisalabad
 2017-06-01 EARLY_PHASE1

Read more about Clinical Trials

Tech Support

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.