Hydroxyurea

Catalog No.S1896 Synonyms: nci-c04831, nsc32065

Hydroxyurea Chemical Structure

Molecular Weight(MW): 76.05

Hydroxyurea is an antineoplastic agent that inhibits DNA synthesis through the inhibition of ribonucleoside diphosphate reductase.

Size Price Stock Quantity  
In DMSO USD 90 In stock
USD 70 In stock
USD 210 In stock
USD 470 In stock
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Biological Activity

Description Hydroxyurea is an antineoplastic agent that inhibits DNA synthesis through the inhibition of ribonucleoside diphosphate reductase.
Targets
ribonucleoside diphosphate reductase [1]
In vitro

hydroxyurea can inhibit HIV-1 replication. In vitro experiments have shown that the 90% inhibitory concentration (IC90) of hydroxyurea for laboratory strains of HIV-1 in activated PBMC is 0.4 mM. Hydroxyurea was also found to be synergistic with the nucleoside reverse transcriptase inhibitor didanosine and to inhibit HIV-1 replication in activated PBMC; this inhibition may be due to a reduction in deoxynucleoside triphosphate pool sizes. Hydroxyurea has been shown to sensitize didanosine-resistant mutants[1][2].hydroxyurea has demonstrated activity in the treatment of sickle cell anemia by increasing the production of fetal hemoglobin, which reduces hemolysis in patients with this disease. Hydroxyurea exerts its cytostatic effect through inhibition of ribonucleotide reductase—the rate-limiting enzyme responsible for the conversion of ribonucleotides to deoxyribonucleotides, which are essential for DNA synthesis. As a result, cellular division is arrested in the S phase[1].

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
L1210 Leukemia cells NV\5XmZ7T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NV36RmVtS2:wY3XueJJifGmxbjDy[ZF2cXKnZDDmc5IhPTBnIHnubIljcXSrb36gc4Yh[2WubDDndo94fGhiKFyxNlExKEyndXvlcYliMSxiSVO1NF0zOS5|N{m2JO69VQ>? MnO3Nlk6OTV{MB?=
P388 leukemic cell NWnYXYFlWHKxbHnm[ZJifGmxbjDhd5NigQ>? NX7GSmdtOjRvNEigbC=> NEXSSohCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBw\iClb33wc5Vv\CCneIDy[ZN{\WRiYYOgZ49v[2WwdILheIlwdiC2aHH0JIlvcGmkaYTzJFUxLSCxZjDndo94fGhib3[gVFM5QCCuZYXr[Y1q[yClZXzsJJN2e3CnboPpc44h\nKxbTCyOEB1dyB2ODDodkBi\nSncjDjc41xd3WwZDDh[IRqfGmxbjygTWM2OD1|MjFOwG0> NXvORms3OjdyOUO3Ni=>

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Protocol

Cell Research:[3]
+ Expand
  • Cell lines: Erythroid cells
  • Concentrations: 30 μM
  • Incubation Time: 96 h
  • Method: Erythroid cells obtained from peripheral blood of the same patients(Thirteen β-Thal/HbE patients are treated with hydroxyurea orally for 2 years at a starting dose of 5 mg/kg/day for 5 days/week with escalation to a maximum of 10 mg/kg/day) 1 year after they had stopped hydroxyurea treatment are treated with hydroxyurea in vitro.Treatment of cells performs in primary culture with 30 μM hydroxyurea for 96 hours.
    (Only for Reference)
Animal Research:[4]
+ Expand
  • Animal Models: Female athymic (nu/nu) nude mice(used for xenograft model)
  • Formulation: saline
  • Dosages: 1500 mg/kg
  • Administration: i.p.
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 15 mg/mL (197.23 mM)
Water 15 mg/mL (197.23 mM)
Ethanol Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 76.05
Formula

CH4N2O2

CAS No. 127-07-1
Storage powder
Synonyms nci-c04831, nsc32065

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Clinical Trial Information

NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT00001197 Completed Sickle Cell Anemia National Heart, Lung, and Blood Institute (NHLBI)|National Institutes of Health Clinical Center (CC) February 7, 1984 Phase 2
NCT01950585 Withdrawn Pulmonary Hypertension National Heart, Lung, and Blood Institute (NHLBI)|The Cleveland Clinic|National Institutes of Health Clinical Center (CC) September 6, 2013 Early Phase 1
NCT03020615 Not yet recruiting Sickle Cell Anemia St. Jude Childrens Research Hospital|National Heart, Lung, and Blood Institute (NHLBI) February 28, 2017 Phase 2
NCT00270478 Completed Sickle Cell Disease|Chronic Kidney Disease|Pulmonary Hypertension National Heart, Lung, and Blood Institute (NHLBI)|National Institutes of Health Clinical Center (CC) December 21, 2005 Phase 1
NCT02675790 Not yet recruiting Sickle Cell Disease|Sickle Cell Anemia|Stroke Vanderbilt University|Aminu Kano Teaching Hospital|Murtala Muhammed Specialist Hospital December 2016 Phase 3
NCT02844673 Enrolling by invitation Sickle Cell Anaemia Muhimbili University of Health and Allied Sciences|University of Pittsburgh|Muhimbili National Hospital October 2016 Phase 4

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID