Disulfiram

Catalog No.S1680 Synonyms: NSC 190940

Disulfiram  Chemical Structure

Molecular Weight(MW): 296.54

Disulfiram is a specific inhibitor of aldehyde-dehydrogenase (ALDH1), used for the treatment of chronic alcoholism by producing an acute sensitivity to alcohol.

Size Price Stock Quantity  
In DMSO USD 130 In stock
USD 97 In stock
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1 Customer Review

  • The effect of drugs on sperm movement. Purified human sperm were incubated under capacitating conditions for 0, 15, 30, 60 or 120 min, and motility was measured in the presence of disulfirum. The standard deviation is shown as bars. Statistical differences by Student's t-test compared with control are annotated as “*” for p<0.05 or “**” for p<0.01.

    J Proteomics 2013 79, 114-22. Disulfiram purchased from Selleck.

Purity & Quality Control

Choose Selective Dehydrogenase Inhibitors

Biological Activity

Description Disulfiram is a specific inhibitor of aldehyde-dehydrogenase (ALDH1), used for the treatment of chronic alcoholism by producing an acute sensitivity to alcohol.
Targets
ALDH1 [1]
In vitro

Disulfiram-copper complex potently inhibits the proteasomal activity in cultured breast cancer MDA-MB-231 and MCF10DCIS.com cells, but not normal, immortalized MCF-10A cells, before induction of apoptotic cancer cell death. [1] Disulfiram (DS), a clinically used anti-alcoholism drug, strongly inhibits constitutive and 5-FU-induced NF-kappaB activity in a dose-dependent manner. Disulfiram inhibits both NF-kappaB nuclear translocation and DNA binding activity but has no effect on 5-FU-induced IkappaBalpha degradation. Disulfiram significantly enhances the apoptotic effect of 5-FU on DLD-1 and RKO(WT) cell lines and synergistically potentiated the cytotoxicity of 5-FU to both cell lines. Disulfiram also effectively abolishes 5-FU chemoresistance in a 5-FU resistant cell line H630(5-FU) in vitro. [2] Oseltamivir decreases the number of viable cells, and the addition of CuCl(2) significantly enhances the DSF-induced cell death to less than 10% of control. [3] Disulfiram given to melanoma cells in combination with Cu2+ or Zn2+ decreases expression of cyclin A and reduces proliferation in vitro at lower concentrations than disulfiram alone. [4]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
MCF7 cells M3zCdHBzd2yrZnXyZZRqd25iYYPzZZk> NYLMeYw5PzJiaB?= NETXSpdCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIF3DSlch[2WubIOg[ZhxemW|c3nu[{BDS0F{IHHu[EBme3S{b3flckBz\WOncITvdkBi\nSncjC3NkBpenNiYomgUXRVKGG|c3H5MEBKSzVyPUCuNUDPxE1? MnH2NlAzOjJ4N{G=
human T47D cells MmLvVJJwdGmoZYLheIlwdiCjc4PhfS=> NGDlPJk4OiCq M3\tcmFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iVES3SEBk\WyuczDlfJBz\XO|aX7nJGJESTJiYX7kJIV{fHKxZ3XuJJJm[2WydH;yJIFnfGW{IEeyJIhzeyCkeTDNWHQh[XO|YYmsJGlEPTB;MD6xO{DPxE1? MWKyNFIzOjZ5MR?=
human MDA-MB-231 cells NVn1SXVJWHKxbHnm[ZJifGmxbjDhd5NigQ>? M{Lo[FczKGh? M3HxcGFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iTVTBMW1DNTJ|MTDj[YxteyCneIDy[ZN{cW6pIFLDRVIh[W6mIFXSZYxxcGFiYX\0[ZIhPzJiaILzJIJ6KE2WVDDhd5NigSxiSVO1NF0xNjN{IN88US=> MonsNlAzOjJ4N{G=
CHO cells MWLGeY5kfGmxbjDhd5NigQ>? NF;ZOFhC\2:waYP0JIFkfGm4aYT5JIF1KGi3bXHuJHRTWEFzIHPoZY5v\WxiZYjwdoV{e2WmIHnuJGNJVyClZXzsd{Bie3Onc4Pl[EBieyCrbnPy[YF{\SCrbjDpcpRz[WOnbHz1cIFzKGOjbHPpeY0hdGW4ZXzzMEBGSzVyPUOg{txO MV6yNFM2PjNyNR?=
human MCF10A cells MW\Qdo9tcW[ncnH0bY9vKGG|c3H5 NYfuPXMyPzJiaB?= MUDBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKE2FRkGwRUBk\WyuczDh[pRmeiB5MjDodpMh[nliTWTUJIF{e2G7LDDJR|UxRTFyIN88US=> M1TBXVIxOjJ{Nkex

... Click to View More Cell Line Experimental Data

In vivo Disulfiram significantly inhibits the tumor growth (by 74%), associated with in vivo proteasome inhibition (as measured by decreased levels of tumor tissue proteasome activity and accumulation of ubiquitinated proteins and natural proteasome substrates p27 and Bax) and apoptosis induction (as shown by caspase activation and apoptotic nuclei formation) in mice bearing MDA-MB-231 tumor xenografts. [1] Disulfiram blocks the P-glycoprotein extrusion pump, inhibits the transcription factor nuclear factor-kappaB, sensitizes tumors to chemotherapy, reduces angiogenesis, and inhibits tumor growth in mice. Disulfiram inhibits growth and angiogenesis in melanomas transplanted in severe combined immunodeficient mice, and these effects are potentiated by Zn2+ supplementation. [4]

Protocol

Solubility (25°C)

In vitro DMSO 59 mg/mL (198.96 mM)
Ethanol 59 mg/mL (198.96 mM)
Water Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 296.54
Formula

C10H20N2S4

CAS No. 97-77-8
Storage powder
Synonyms NSC 190940

Bio Calculators

Molarity Calculator

Molarity Calculator

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Dilution Calculator

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This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

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* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

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Definitions of molecular mass, molecular weight, molar mass and molar weight:

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Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

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Clinical Trial Information

NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT03034135 Not yet recruiting Recurrent Glioblastoma Cantex Pharmaceuticals January 31, 2017 Phase 2
NCT02963051 Not yet recruiting Prostate Cancer Daniel George, MD|Give 1 For Dad Campaign|The V Foundation for Cancer Research|Peter Michael Foundation|Duke University February 2017 Phase 1
NCT01777919 Not yet recruiting Glioblastoma Multiforme Olympion Medical Center|University of Ioannina|University of Eastern Finland|University of Ulm January 2017 Phase 2
NCT02678975 Recruiting Glioma|Glioblastoma Sahlgrenska University Hospital, Sweden|St. Olavs Hospital|Lund University Hospital|Karolinska University Hospital|University Hospital, Linkoeping January 2017 Phase 2|Phase 3
NCT02770378 Recruiting Glioblastoma University of Ulm|Reliable Cancer Therapies|Anticancer Fund, Belgium November 2016 Phase 1
NCT02735577 Recruiting Alcohol Use Disorder New York State Psychiatric Institute March 2016 Phase 4

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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Dehydrogenase Signaling Pathway Map

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID