Home Microbiology Ribosomal Peptidyl Transferase inhibitor Tiamulin

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Tiamulin Ribosomal Peptidyl Transferase inhibitor

Cat.No.S5040

Tiamulin is a semisynthetic pleuromutilin antibiotic that binds to the ribosomal peptidyl transferase centre and inhibits protein synthesis. This compound has sustained antibacterial activity against specified bacterial species and rapidly growing and fastidious animal pathogens.
Tiamulin Ribosomal Peptidyl Transferase inhibitor Chemical Structure

Chemical Structure

Molecular Weight: 493.74

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Quality Control

Batch: S504001 DMSO]98 mg/mL]false]Water]98 mg/mL]false]Ethanol]98 mg/mL]false Purity: 98%
98

Solubility

In vitro
Batch:

DMSO : 98 mg/mL (198.48 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)

Water : 98 mg/mL

Ethanol : 98 mg/mL

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In vivo
Batch:

In vivo Formulation Calculator (Clear solution)

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Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Chemical Information, Storage & Stability

Molecular Weight 493.74 Formula

C28H47NO4S

 Storage (From the date of receipt)
CAS No. 55297-95-5 -- Storage of Stock Solutions

Synonyms N/A Smiles CCN(CC)CCSCC(=O)OC1CC(C(C(C23CCC(C1(C2C(=O)CC3)C)C)C)O)(C)C=C

Mechanism of Action

In vitro
Tiamulin has a remarkable effect on decreasing the activity of CD73 in vitro and dramatically inhibits colony formation of MDA-MB-231 and 4 T1 cells. This compound is located within the peptidyl transferase center (PTC) of the 50S ribosomal subunit with its tricyclic mutilin core positioned in a tight pocket at the A-tRNA binding site. It directly inhibits peptide bond formation. It interacts with 23S rRNA and clearly interferes with the correct positioning of both A- and P-site substrates.
In vivo
Tiamulin inhibits breast tumor growth and metastasis, mainly achieved by affecting the activity but not expression of CD73. The antitumor effect of this compound results in inhibition of metastasis and angiogenesis.
References

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