Tat-beclin 1 (Tat-BECN1)

Catalog No.S8595

For research use only.

Tat-beclin 1 (Tat-BECN1), a peptide known to stimulate autophagy through mobilization of endogenous Beclin 1, induces autophagy in vitro and in vivo and improves clinical outcomes.

Tat-beclin 1 (Tat-BECN1) Chemical Structure

CAS No. 1423821-88-8

Selleck's Tat-beclin 1 (Tat-BECN1) has been cited by 6 Publications

Purity & Quality Control

Choose Selective Autophagy Inhibitors

Biological Activity

Description Tat-beclin 1 (Tat-BECN1), a peptide known to stimulate autophagy through mobilization of endogenous Beclin 1, induces autophagy in vitro and in vivo and improves clinical outcomes.
Targets
Autophagy [1]
()
In vitro

Tat-beclin 1 is a cell-permeable peptide that induces autophagy. Treatment with Tat-beclin 1, but not Tat-scrambled, results in a dose-dependent decrease in amounts of p62, a selective autophagy substrate, and a dose-dependent conversion of the non-lipidated form of LC3, LC3-I, to the lipidated, autophagosome-associated form of LC3, LC3-II, in multiple cell lines and primary murine embryonic fibroblasts (MEFs). Tat-beclin 1 markedly inhibits HIV-1 replication in primary human monocyte-derived macrophages[1].

Assay
Methods Test Index PMID
Western blot LC3A-II / LC3B-II ; POU5F1 / NANOG / SOX2 ; TUBB3 / CSN2 27929731
In vivo Tat-beclin 1 can induce autophagy in peripheral tissues in adult mice as well as in the central nervous system of neonatal mice. Daily Tat-beclin 1 peptide treatment is well-tolerated in both neonatal and adult mice. Tat-beclin 1 peptide improves the clinical outcome of mice with CNS WNV(West Nile virus) infection[1].

Protocol (from reference)

Cell Research:

[1]

  • Cell lines: human MDMs
  • Concentrations: 0.5–5 μM
  • Incubation Time: 24 h
  • Method:

    For treatment of primary human MDMs, cells are washed with PBS and pre-treated with peptides (0.5-5 μM, 24 h) in 500 μl macrophage-SFM (serum-free media) before infection with HIV-1.

Animal Research:

[1]

  • Animal Models: C57BL/6J mice
  • Dosages: 15 mg/kg
  • Administration: i.p.

Solubility (25°C)

In vitro

Water 100 mg/mL
(26.73 mM)


Chemical Information

Molecular Weight 3741.1
Formula

C164H251N57O45

CAS No. 1423821-88-8
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles CCC(C)C(NNC(CC1=C[NH]C2=C1C=CC=C2)C(=O)NC(CC3=CN=C[NH]3)C(=O)NC(CC(O)=O)C(=O)NCC(=O)NC(CCC(O)=O)C(=O)NC(CC4=CC=CC=C4)C(=O)NCC(=O)NC(C(C)O)C(O)=O)C(=O)NC(CCC(O)=O)C(=O)NC(CC5=CC=CC=C5)C(=O)C(=O)C(NC(=O

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

mg/kg g μL

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO % % Tween 80 % ddH2O
%DMSO %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
If you have any other enquiries, please leave a message.

* Indicates a Required Field

Please enter your name.
Please enter your email. Please enter a valid email address.
Please write something to us.
Tags: buy Tat-beclin 1 (Tat-BECN1) | Tat-beclin 1 (Tat-BECN1) supplier | purchase Tat-beclin 1 (Tat-BECN1) | Tat-beclin 1 (Tat-BECN1) cost | Tat-beclin 1 (Tat-BECN1) manufacturer | order Tat-beclin 1 (Tat-BECN1) | Tat-beclin 1 (Tat-BECN1) distributor