Puromycin aminonucleoside

For research use only.

Catalog No.S9631 Synonyms: NSC 3056, PAN, Stylomycin aminonucleoside, ARDMA, SAN

Puromycin aminonucleoside Chemical Structure

CAS No. 58-60-6

Puromycin aminonucleoside (NSC 3056, PAN, Stylomycin aminonucleoside, ARDMA, SAN), the aminonucleoside portion of the antibiotic puromycin, is a reversible inhibitor of dipeptidyl-peptidase II and cytosol alanyl aminopeptidase that induces apoptosis in mesangial cells (MCs) accompanied by declined cell viability and enhanced inflammatory response.

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Biological Activity

Description Puromycin aminonucleoside (NSC 3056, PAN, Stylomycin aminonucleoside, ARDMA, SAN), the aminonucleoside portion of the antibiotic puromycin, is a reversible inhibitor of dipeptidyl-peptidase II and cytosol alanyl aminopeptidase that induces apoptosis in mesangial cells (MCs) accompanied by declined cell viability and enhanced inflammatory response.
Targets
dipeptidyl-peptidase II [1]
()
cytosol alanyl aminopeptidase [1]
()
In vitro

Puromycin aminonucleoside (PAN) induces MC apoptosis accompanied by the declined cell viability and enhanced inflammatory response. In PAN-treated MCs, ERRα overexpression further aggravates PAN-induced apoptosis.[1]

In vivo

In agreement with the in vitro study, increased ERRα expression is observed in line with enhanced apoptotic response in renal cortex from PAN-treated rats.[1]

Protocol

Cell Research:

[1]

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  • Cell lines: mouse mesangial cells
  • Concentrations: 50 μg/ml, 100 μg/ml
  • Incubation Time: 24 h
  • Method:

    For apoptosis assay, mesangial cells are treated with PAN for 24 h at the indicated concentration. After treatment, cells are stained with FITC-Annexin V and PI according to the manufacturer’s protocol. Cell apoptosis is determined using a BD FACS Calibur flow cytometer and data is analyzed with Flowjo software.


    (Only for Reference)
Animal Research:

[1]

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  • Animal Models: male Sprague-Dawley rats
  • Dosages: 150 mg/kg
  • Administration: IV
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 59 mg/mL (200.46 mM)
Water 30 mg/mL (101.93 mM)
Ethanol Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 294.31
Formula

C12H18N6O3

CAS No. 58-60-6
Storage powder
in solvent
Synonyms NSC 3056, PAN, Stylomycin aminonucleoside, ARDMA, SAN
Smiles CN(C)C1=NC=NC2=C1N=CN2C3C(C(C(O3)CO)N)O

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Clinical Trial Information

NCT Number Recruitment interventions Conditions Sponsor/Collaborators Start Date Phases
NCT05014360 Not yet recruiting Drug: JNJ-64251330 Adenomatous Polyposis Coli Janssen Research & Development LLC November 1 2021 Phase 1
NCT04798612 Not yet recruiting Drug: Pegasys|Drug: Saline Colon Cancer Zealand University Hospital October 1 2021 Phase 2
NCT04989231 Not yet recruiting Biological: Investigational sIPV|Biological: Control IPV Poliomyelitis Sinovac Biotech Co. Ltd September 25 2021 --

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID