Name: Pimonidazole
Brand: Selleck Chemicals
SKU: S9919
Availability: InStock
Rating: 5 (1 reviews)

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Quality Control

Batch: S991901 DMSO]51 mg/mL]false]Ethanol]51 mg/mL]false]Water]Insoluble]false Purity: 99.52%

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COA
NMR
HPLC
SDS/MSDS
Datasheet

99.52

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Solubility

In vitro
Batch:

DMSO : 51 mg/mL (200.55 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)

Ethanol : 51 mg/mL

Water : Insoluble

Molarity Calculator

Mass	Concentration	Volume	Molecular Weight
Mass value Mass unit	Concentration value Concentration unit	Volume value Volume unit	Molecular weight (g/mol)

Dilution Calculator Molecular Weight Calculator

In vivo batch selection In vivo Batch:
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution	5%DMSO 1 95 %Corn oil Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	0.42mg/ml (1.65mM)	Taking the 1 mL working solution as an example, add 50 μL of 8.4 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.
Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	2.95mg/ml (11.60mM)	Taking the 1 mL working solution as an example, add 50 μL of 59 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg

Average weight of animals: g

Dosing volume per animal: μL

Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO

%

% Tween 80

% ddH₂O

% DMSO

+

%

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Chemical Information, Storage & Stability

Molecular Weight	254.29	Formula	C₁₁H₁₈N₄O₃	Storage (From the date of receipt)	3 years -20°C powder
CAS No.	70132-50-2	--		Storage of Stock Solutions	1 year-80°Cin solvent 1 month-20°Cin solvent
Synonyms	Ro 03-8799			SMILES	OC(CN1CCCCC1)C[N]2C=CN=C2[N+]([O-])=O

Read more about storage stability stock solution CAS number SMILES

Mechanism of Action

In vitro	Pimonidazole has radio-sensitivity and recovery capacity from radiation-induced damage depend on p53 status of the tumor cell.<sup><a class="sref" href="#s_ref">[3]</a></sup>
In vivo	Pimonidazole can overcome radiation resistance in hypoxiaby melanoma cells, which is enhanced by liposome modification.<sup><a class="sref" href="#s_ref">[2]</a></sup>
References	[1] https://pubmed.ncbi.nlm.nih.gov/9826471/ [2] https://pubmed.ncbi.nlm.nih.gov/27580239/ [3] https://pubmed.ncbi.nlm.nih.gov/22905487/

Clinical Trial Information

(data from https://clinicaltrials.gov, updated on 2026-08-10)

NCT Number	Recruitment	Conditions	Sponsor/Collaborators	Start Date	Phases
NCT03418818	ACTIVE_NOT_RECRUITING	Sarcoma	University Health Network, Toronto	2018-03-27
NCT02095249	ACTIVE_NOT_RECRUITING	Prostate Cancer	University Health Network, Toronto	2014-09
NCT06085781	RECRUITING	Squamous Cell Carcinoma of Head and Neck	University Health Network, Toronto	2024-01-29
NCT03410420	COMPLETED	Aortic Aneurysm, Thoracic	Thomas Gleason	2018-09-01	PHASE1
NCT03181035	SUSPENDED	Tongue Cancer	Sunnybrook Health Sciences Centre	2018-01-25	PHASE1; PHASE2
NCT02414048	UNKNOWN	Head and Neck Cancer	Marius Gustav Bredell	2015-04	PHASE2

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Pimonidazole

Chemical Structure

Molecular Weight: 254.29