NVP-2

For research use only.

Catalog No.S8981

NVP-2 Chemical Structure

CAS No. 1263373-43-8

NVP-2, a potent, selective, non-neurotoxic and ATP-competitive cyclin dependent kinase 9 (CDK9) inhibitor with IC50 of 0.514 nM for CDK9/CycT activity and induces cell apoptosis.

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Biological Activity

Description NVP-2, a potent, selective, non-neurotoxic and ATP-competitive cyclin dependent kinase 9 (CDK9) inhibitor with IC50 of 0.514 nM for CDK9/CycT activity and induces cell apoptosis.
Targets
CDK9/CycT [1]
(Cell-free assay)
0.514 nM
In vitro

NVP-2 selectively inhibits CDK9. NVP-2 exhibits anti-proliferative effects and transcription inhibition. NVP-2 diminishes elongating polymerase II.[1]

Protocol

Cell Research:

[1]

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  • Cell lines: Wildtype MOLT4 Cells, CRBN−/− MOLT4 Cells
  • Concentrations: 1μM, 500nM, 250nM
  • Incubation Time: 6 h, 72 h
  • Method:

    Proliferation assays are performed by treating MOLT4 and CRBN−/− MOLT4 cells with NVP-2 at concentrations indicated for 72 hours. Anti-proliferative effects of NVP-2 are assessed using Cell Titer Glo assay kit. IC50s are determined using Graphpad Prism non-linear regression curve fit. To determine the effect of washout, MOLT4 and CRBN−/− MOLT4 cells are treated with 250 nM NVP-2 or DMSO. After 6 hours, compound is washed from cells by washing cells with media three times where indicated. Cells are allowed to grow for another 66 hours (72-hour total) and assayed for anti-proliferation using Cell Titer Glo assay kit as above.


    (Only for Reference)

Solubility (25°C)

In vitro DMSO 100 mg/mL (194.9 mM)
Ethanol 100 mg/mL (194.9 mM)
Water Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 513.07
Formula

C27H37ClN6O2

CAS No. 1263373-43-8
Storage powder
in solvent
Synonyms N/A
Smiles CC(COC)NC1CCC(CC1)NC2=NC=C(C(=C2)C3=NC(=CC=C3)NCC4(CCOCC4)C#N)Cl

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Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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CDK Signaling Pathway Map

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID