Nomilin

Nomilin is a triterpenoid present in common edible citrus fruits with putative anticancer properties.

Nomilin Chemical Structure

Nomilin Chemical Structure

CAS: 1063-77-0

Purity & Quality Control

Batch: S504501 DMSO] 100 mg/mL] false] Water] Insoluble] false] Ethanol] Insoluble] false Purity: 99.59%
99.59

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Biological Activity

Description Nomilin is a triterpenoid present in common edible citrus fruits with putative anticancer properties.
In vitro
In vitro Administration of nomilin significantly retarded endothelial cell proliferation, migration, invasion and tube formation. It also possesses anti-proliferative activity against number of human cancer cell lines including leukemia (HL-60), ovary (SKOV-3), cervix (HeLa), stomach (NCI-SNU-1), liver (Hep G2), and breast (MCF-7)[2]. Nomilin significantly decreased TRAP-positive multinucleated cell numbers compared with the control, and exhibited no cytotoxicity. It decreases bone resorption activity and downregulates osteoclast-specific genes, NFATc1 and TRAP mRNA levels. Furthermore, nomilin suppressed MAPK signaling pathways. Thus, nomilin has inhibitory effects on osteoclastic differentiation in vitro[3].
Cell Research Cell lines Human umbilical vein endothelial cells (HUVECs)
Concentrations 5 μg-500 μg/ml
Incubation Time 48 h
Method

HUVECs were seeded (5000 cells/well) in 96-well flat bottomed titer plate and incubated for 24 h at 37 °C in 5% CO2 atmosphere. Different concentrations of nomilin (5 μg-500 μg/ml) were added and incubated further for 48 h. Before 4 h completion of incubation, 20 μl MTT (5 mg/ml) was added. Percentage of dead cells was determined using an ELISA plate reader set to record absorbance at 570 nm.

In Vivo
In vivo Apart from antifeedant activity, Nomilin is a potent inducers of gluthathione S-transferase activity in mice and to inhibit carcinogenesis in the hamster buccal pouch assay. Nomilin can shorten anaesthetic-induced sleeping time in mice, probably through a stimulant activity on the central nervous system[1]. Nomilin significantly inhibited tumor directed capillary formation. Serum proinflammatory cytokines such as IL-1β, IL-6, TNF-α and GM-CSF and also serum NO levels were significantly reduced by the treatment of nomilin. Administration of nomilin significantly reduced the serum level of VEGF, a proangiogenic factor and increased the antiangiogenic factors IL-2 and TIMP-1[2].
Animal Research Animal Models Four to six week old male C57BL/6 mice
Dosages 6 mg/kg
Administration i.p.

Chemical Information & Solubility

Molecular Weight 514.56 Formula

C28H34O9

CAS No. 1063-77-0 SDF --
Smiles CC(=O)OC1CC(=O)OC(C2C1(C3CCC4(C(OC(=O)C5C4(C3(C(=O)C2)C)O5)C6=COC=C6)C)C)(C)C
Storage (From the date of receipt)

In vitro
Batch:

DMSO : 100 mg/mL ( (194.34 mM); Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : Insoluble

Ethanol : Insoluble


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In vivo
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Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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