Nomilin

Catalog No.S5045 Batch:S504501

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Technical Data

Formula

C28H34O9
Molecular Weight 514.56 CAS No. 1063-77-0
Solubility (25°C)* In vitro DMSO 100 mg/mL (194.34 mM)
Water Insoluble
Ethanol Insoluble
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

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Biological Activity

Description Nomilin is a triterpenoid present in common edible citrus fruits with putative anticancer properties.
In vitro Administration of nomilin significantly retarded endothelial cell proliferation, migration, invasion and tube formation. It also possesses anti-proliferative activity against number of human cancer cell lines including leukemia (HL-60), ovary (SKOV-3), cervix (HeLa), stomach (NCI-SNU-1), liver (Hep G2), and breast (MCF-7)[2]. Nomilin significantly decreased TRAP-positive multinucleated cell numbers compared with the control, and exhibited no cytotoxicity. It decreases bone resorption activity and downregulates osteoclast-specific genes, NFATc1 and TRAP mRNA levels. Furthermore, nomilin suppressed MAPK signaling pathways. Thus, nomilin has inhibitory effects on osteoclastic differentiation in vitro[3].
In vivo Apart from antifeedant activity, Nomilin is a potent inducers of gluthathione S-transferase activity in mice and to inhibit carcinogenesis in the hamster buccal pouch assay. Nomilin can shorten anaesthetic-induced sleeping time in mice, probably through a stimulant activity on the central nervous system[1]. Nomilin significantly inhibited tumor directed capillary formation. Serum proinflammatory cytokines such as IL-1β, IL-6, TNF-α and GM-CSF and also serum NO levels were significantly reduced by the treatment of nomilin. Administration of nomilin significantly reduced the serum level of VEGF, a proangiogenic factor and increased the antiangiogenic factors IL-2 and TIMP-1[2].

Protocol (from reference)

Cell Assay:

[2]
  • Cell lines

    Human umbilical vein endothelial cells (HUVECs)

  • Concentrations

    5 μg-500 μg/ml

  • Incubation Time

    48 h

  • Method

    HUVECs were seeded (5000 cells/well) in 96-well flat bottomed titer plate and incubated for 24 h at 37 °C in 5% CO2 atmosphere. Different concentrations of nomilin (5 μg-500 μg/ml) were added and incubated further for 48 h. Before 4 h completion of incubation, 20 μl MTT (5 mg/ml) was added. Percentage of dead cells was determined using an ELISA plate reader set to record absorbance at 570 nm.
Animal Study:

[2]
  • Animal Models

    Four to six week old male C57BL/6 mice

  • Dosages

    6 mg/kg

  • Administration

    i.p.

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.