NIBR189

Catalog No.S0183

For research use only.

NIBR189 is a potent antagonist of the Epstein-Barr virus-induced gene 2 (EBI2, GPR183) receptor with IC50 of 16 nM for binding and 11 nM for function, respectively.

NIBR189 Chemical Structure

CAS No. 1599432-08-2

Purity & Quality Control

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Biological Activity

Description NIBR189 is a potent antagonist of the Epstein-Barr virus-induced gene 2 (EBI2, GPR183) receptor with IC50 of 16 nM for binding and 11 nM for function, respectively.
Targets
EBI2 (function) [1]
(Cell-free assay)
EBI2 (binding) [1]
(Cell-free assay)
11 nM 16 nM
In vitro

NIBR189, the EBI2 antagonist, blocks the migration of U937 cells expressing functional EBI2 levels with an IC50 of 0.3 nM.[1]

In vivo

NIBR189 is a potent and selective EBI2 antagonist with pharmacokinetic properties which should allow use for in vitro and in vivo experiments.[1]

Protocol (from reference)

Cell Research:

[1]

  • Cell lines: U937 cells
  • Concentrations: 20 nM
  • Incubation Time: 3 h
  • Method:

    Prior to the migration assay, U937 cells were kept overnight in lipid-depleted media. Chemotaxis was performed using HTS Transwell-96 plates with 5.0 μm pore polycarbonate membranes. In brief, lower chambers were filled with 240 μL of compound solution in media in which the FCS was substituted with 1% BSA, and after insertion of the filters, 0.75×105 cells in 75 μL media were added to the upper chamber. After 3 h at 37℃, cells in the lower chamber were analyzed by flow cytometry.

Animal Research:

[1]

  • Animal Models: C57BL/6 male mice
  • Dosages: 1 mg/kg (i.v.); 3 mg/kg (p.o.)
  • Administration: i.v., p.o.

Solubility (25°C)

In vitro

Chemical Information

Molecular Weight 429.31
Formula

C21H21BrN2O3

CAS No. 1599432-08-2
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles COC1=CC=C(C=C1)C(=O)N2CCN(CC2)C(=O)C=CC3=CC=C(C=C3)Br

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Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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