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MPA (Medroxyprogesterone acetate) Estrogen/progestogen Receptor agonist

Name: MPA (Medroxyprogesterone acetate)
Brand: Selleck Chemicals
SKU: S2567
Availability: InStock
Rating: 5 (16 reviews)

Cat.No.S2567

Medroxyprogesterone acetate (MPA) is a synthetic progestin that acts as a potent progesterone receptor agonist, used to treat abnormal menstruation or irregular vaginal bleeding.

Chemical Structure

Molecular Weight: 386.52

Jump to Mechanism of Action Solubility Chemical Information, Storage & Stability Specificity

Quality Control

Batch: Purity: 99.98%

99.98

Related Targets	Adrenergic Receptor GPR Androgen Receptor Glucocorticoid Receptor ACE RAAS Progesterone Receptor Opioid Receptor THR PGES Aromatase CCK receptor 5-alpha Reductase Mineralocorticoid Receptor GHSR SSTR TSH Receptor GNRH Receptor PGDS
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Chemical Information, Storage & Stability

Molecular Weight	386.52	Formula	C₂₄H₃₄O₄	Storage (From the date of receipt)	3 years-20°Cpowder
CAS No.	71-58-9	Download SDF		Storage of Stock Solutions	1 year-80°Cin solvent 1 month-20°Cin solvent
Synonyms	NSC-26386, Medroxyprogesterone 17-acetate, Farlutin			Smiles	CC1CC2C(CCC3(C2CCC3(C(=O)C)OC(=O)C)C)C4(C1=CC(=O)CC4)C

Solubility

In vitro
Batch:

DMSO : 12 mg/mL (31.04 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)

Ethanol : 12 mg/mL

Water : Insoluble

Molarity Calculator

Mass	Concentration	Volume	Molecular Weight
Mass value Mass unit	Concentration value Concentration unit	Volume value Volume unit	Molecular weight (g/mol)

Dilution Calculator Molecular Weight Calculator

In vivo batch selection In vivo Batch:
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution	5%DMSO 1 30%PEG300 2 5%Tween80 3 60%ddH2O Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	0.500mg/ml (1.29mM)	Taking the 1 mL working solution as an example, add 50 μL of 10 mg/ml clarified DMSO stock solution to 300 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 600 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg

Average weight of animals: g

Dosing volume per animal: μL

Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO

% Tween 80

% ddH₂O

% DMSO

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Mechanism of Action

Targets/IC₅₀/Ki	progestogen Receptor ^[1]
In vitro	Medroxyprogesterone acetate (MPA) inhibits the enzyme 3-hydroxysteroid dehydrogenase, involved in the reversible conversion between 5alpha-dihydroprogesterone (DHP) and 3alpha, 5alpha-tetrahydroprogesterone (THP), and therefore may affect the local actions of DHP and THP in the brain. ^[1] It reduces secretion of IL-6 and PTHrP from human breast cancer cells. This compound dose-dependently decreases the secretion and mRNA expression of IL-6 and PTHrP in the KTC-2 cells. ^[2] MPA and dexamethasone dose dependently increase alpha-ENaC promoter-driven luciferase activity in M-1 cells, which is not inhibited by Org31710, indicating that it regulates alpha-ENaC in a PR-independent manner. This compound and dexamethasone, but not progesterone, dose dependently increase alpha-ENaC and sgk1 mRNA in M-1 and in Madin-Darby canine kidney-C7 cells, both collecting duct cell lines. ^[3] At 0.1 nM, it significantly enhances the in vitro production of specific immunoglobulin G (IgG) antibodies, an effect that appears to involve the interaction of the progestin with PRG receptors^[4]
In vivo	Medroxyprogesterone acetate (MPA) impairs delayed memory retention on the water radial-arm maze (WRAM) and exacerbates overnight forgetting on the Morris maze (MM) in aged ovariectomized (OVX) rats. It significantly and progesterone marginally decreases GAD levels in the hippocampus, while both MPA and progesterone significantly increase GAD levels in the entorhinal cortex. ^[5]
References	[1] https://pubmed.ncbi.nlm.nih.gov/16758493/ [2] https://pubmed.ncbi.nlm.nih.gov/12729473/ [3] https://pubmed.ncbi.nlm.nih.gov/16189295/ [4] https://pubmed.ncbi.nlm.nih.gov/11576224/ [5] https://pubmed.ncbi.nlm.nih.gov/20074654/ [6] https://pubmed.ncbi.nlm.nih.gov/33937078/

Clinical Trial Information

(data from https://clinicaltrials.gov, updated on 2024-05-22)

NCT Number	Recruitment	Conditions	Sponsor/Collaborators	Start Date	Phases
NCT05178563	Not yet recruiting	Inflammation\|Periodontal Diseases	University College London	September 1 2024	Not Applicable
NCT06297096	Not yet recruiting	Systemic Sclerosis\|Interstitial Lung Disease	National Institute of Geriatrics Rheumatology and Rehabilitation Poland\|Medical Research Agency Poland	April 1 2024	Phase 3
NCT06193135	Not yet recruiting	Infertility Female\|Reproductive Sterility	Instituto Valenciano de Infertilidad IVI VALENCIA	March 1 2024	Not Applicable

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