Name: Hemin
Brand: Selleck Chemicals
SKU: S5645
Rating: 5 (6 reviews)

Hemin (protohemin) is an iron-containing porphyrin used in the management of porphyria attacks, particularly in acute intermittent porphyria.Hemin is an Heme oxygenase (HO)-1 inducer.This product has poor solubility, animal experiments are available, cell experiments please choose carefully!

Hemin Chemical Structure

CAS: 16009-13-5

Selleck's Hemin has been cited by 6 publications

Purity & Quality Control

Batch: Purity: 99.53%

99.53

Hemin Related Products

Related Targets	HO-1	Click to Expand
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Biological Activity

Description	Hemin (protohemin) is an iron-containing porphyrin used in the management of porphyria attacks, particularly in acute intermittent porphyria.Hemin is an Heme oxygenase (HO)-1 inducer.This product has poor solubility, animal experiments are available, cell experiments please choose carefully!

In vitro
In vitro	Treatment of hemin or AFC damped the expression of TFRC in CT26 cells, which can be significantly rescued by overexpression of OTUD1.^[1]
Cell Research	Cell lines	CT26 cells
	Concentrations	100 μM
	Incubation Time	30 min
	Method	Mock‐ and OTUD1‐expressing CT26 cells are treated with hemin (100 μM). Flow cytometric analyses the TFRC expression.

In Vivo
In vivo	Following the i.p. administration of Hemin (100 μM/kg), the HO-1 level in the renal cortex begins to increase gradually. The HO-1 level reaches its peak 24 h after Hemin preconditioning. HO-1 is expressed mainly in the renal tubules. The HO-2 level in the kidney does not change following Hemin preconditioning.

NCT Number	Recruitment	Conditions	Sponsor/Collaborators	Start Date	Phases
Clinical Trial Information (data from https://clinicaltrials.gov, updated on 2024-01-11)
NCT03338816	Completed	Acute Hepatic Porphyria\|Acute Intermittent Porphyria\|Porphyria Acute Intermittent\|Acute Porphyria\|Hereditary Coproporphyria (HCP)\|Variegate Porphyria (VP)\|ALA Dehydratase Deficient Porphyria (ADP)	Alnylam Pharmaceuticals	November 16 2017	Phase 3
NCT01855841	Completed	Post-ERCP Acute Pancreatitis	Erasme University Hospital	April 2012	Phase 2

References

[1] Song J, et al. EMBO Rep. 2021 Feb 3;22(2):e51162.

Chemical Information & Solubility

Molecular Weight	651.94	Formula	C₃₄H₃₂ClFeN₄O₄
CAS No.	16009-13-5	SDF	--
Smiles	CC1=C(C2=CC3=C(C(=C([N-]3)C=C4C(=C(C(=N4)C=C5C(=C(C(=N5)C=C1[N-]2)C)C=C)C)C=C)C)CCC(=O)O)CCC(=O)O.[Cl-].[Fe+3]
Storage (From the date of receipt)	3 years -20°C(in the dark) powder	Storage of Stock Solutions Aliquot the stock solutions to avoid repeated freeze-thaw cycles	1 year-80°Cin solvent
Storage (From the date of receipt)	3 years -20°C(in the dark) powder		1 month-20°Cin solvent

In vitro
Batch:

DMSO : 20 mg/mL ( (30.67 mM)； Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : Insoluble

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In vivo
Batch:

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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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