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Hemin Heme Oxygenase chemical

Name: Hemin
Brand: Selleck Chemicals
SKU: S5645
Availability: InStock
Rating: 5 (8 reviews)

Cat.No.S5645

Hemin (protohemin) is an iron-containing porphyrin used in the management of porphyria attacks, particularly in acute intermittent porphyria.This compound is an Heme oxygenase (HO)-1 inducer.This product has poor solubility, animal experiments are available, cell experiments please choose carefully!

Chemical Structure

Molecular Weight: 651.94

Jump to Mechanism of Action Solubility Chemical Information, Storage & Stability

Quality Control

Batch: Purity: 99.53%

99.53

Related Targets	Apoptosis related Ras STAT TNF-alpha Bcl-2 Caspase PD-1/PD-L1 Ferroptosis p53 RIP kinase c-RET MDM2/MDMX Myc IAP OXPHOS PERK Thymidylate Synthase FKBP PFKFB PKD Survivin Pyroptosis ASK Bcl-6 Nur77 DAPK TRADD cytohesin TACE Cuproptosis
Related Products	Fraxetin

Chemical Information, Storage & Stability

Molecular Weight	651.94	Formula	C₃₄H₃₂ClFeN₄O₄	Storage (From the date of receipt)	3 years -20°C(in the dark) powder
CAS No.	16009-13-5	--		Storage of Stock Solutions	1 year-80°Cin solvent 1 month-20°Cin solvent
Synonyms	protohemin			Smiles	CC1=C(C2=CC3=C(C(=C([N-]3)C=C4C(=C(C(=N4)C=C5C(=C(C(=N5)C=C1[N-]2)C)C=C)C)C=C)C)CCC(=O)O)CCC(=O)O.[Cl-].[Fe+3]

Solubility

In vitro
Batch:

DMSO : 20 mg/mL ( (30.67 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : Insoluble

Molarity Calculator

Mass	Concentration	Volume	Molecular Weight
=	×	×

Dilution Calculator Molecular Weight Calculator

In vivo Batch:
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	1.000mg/ml (1.53mM)	Taking the 1 mL working solution as an example, add 50 μL of 20 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Clear solution	5% DMSO 1 95% Corn oil Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	1.000mg/ml (1.53mM)	Taking the 1 mL working solution as an example, add 50 μL of 20 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg Average weight of animals: g Dosing volume per animal:μL Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO + % + % Tween 80 + % ddH₂O

%DMSO+ %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Mechanism of Action

In vitro	Treatment of hemin or AFC damped the expression of TFRC in CT26 cells, which can be significantly rescued by overexpression of OTUD1.^[1]
In vivo	Following the i.p. administration of Hemin (100 μM/kg), the HO-1 level in the renal cortex begins to increase gradually. The HO-1 level reaches its peak 24 h after this compound preconditioning. HO-1 is expressed mainly in the renal tubules. The HO-2 level in the kidney does not change following this chemical preconditioning.
References	https://pubmed.ncbi.nlm.nih.gov/33393230/

Clinical Trial Information

(data from https://clinicaltrials.gov, updated on 2024-05-22)

NCT Number	Recruitment	Conditions	Sponsor/Collaborators	Start Date	Phases
NCT03338816	Completed	Acute Hepatic Porphyria\|Acute Intermittent Porphyria\|Porphyria Acute Intermittent\|Acute Porphyria\|Hereditary Coproporphyria (HCP)\|Variegate Porphyria (VP)\|ALA Dehydratase Deficient Porphyria (ADP)	Alnylam Pharmaceuticals	November 16 2017	Phase 3
NCT02949830	Completed	Acute Intermittent Porphyria	Alnylam Pharmaceuticals	October 2016	Phase 1\|Phase 2
NCT01855841	Completed	Post-ERCP Acute Pancreatitis	Erasme University Hospital	April 2012	Phase 2

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