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Deoxycholic acid GPCR19 activator

Name: Deoxycholic acid
Brand: Selleck Chemicals
SKU: S4689
Availability: InStock
Rating: 5 (5 reviews)

Cat.No.S4689

Deoxycholic acid (Deoxycholate, Desoxycholic acid, Cholerebic, Cholorebic) is a cytolytic agent. The physiologic effect of this compound is by means of decreased cell membrane integrity. It is specifically responsible for activating the G protein-coupled bile acid receptor TGR5 that stimulates brown adipose tissue (BAT) thermogenic activity.

Chemical Structure

Molecular Weight: 392.57

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Quality Control

Batch: S468901 DMSO]78 mg/mL]false]Ethanol]60 mg/mL]false]Water]Insoluble]false Purity: 99.94%

Cited in Nature Medicine for its top-tier quality
COA
NMR
SDS/MSDS
Datasheet

99.94

Related Targets	CXCR Hedgehog/Smoothened PKA Adrenergic Receptor AChR 5-HT Receptor Histamine Receptor Dopamine Receptor Ras KRas
Other GPCR19 Inhibitors	SBI-115 TGR5 Receptor Agonist BAR501 INT-777 SB-756050

Solubility

In vitro
Batch:

DMSO : 78 mg/mL (198.69 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)

Ethanol : 60 mg/mL

Water : Insoluble

Molarity Calculator

Mass	Concentration	Volume	Molecular Weight
Mass value Mass unit	Concentration value Concentration unit	Volume value Volume unit	Molecular weight (g/mol)

Dilution Calculator Molecular Weight Calculator

In vivo batch selection In vivo Batch:
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution	5%DMSO 1 Corn oil Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	3.900mg/ml (9.93mM)	Taking the 1 mL working solution as an example, add 50 μL of 78 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg

Average weight of animals: g

Dosing volume per animal: μL

Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO

% Tween 80

% ddH₂O

% DMSO

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Chemical Information, Storage & Stability

Molecular Weight	392.57	Formula	C₂₄H₄₀O₄	Storage (From the date of receipt)	3 years-20°Cpowder
CAS No.	83-44-3	Download SDF		Storage of Stock Solutions	1 year-80°Cin solvent 1 month-20°Cin solvent
Synonyms	Deoxycholate, Desoxycholic acid, Cholerebic, Cholorebic			SMILES	CC(CCC(=O)O)C1CCC2C1(C(CC3C2CCC4C3(CCC(C4)O)C)O)C

Read more about storage stability stock solution CAS number SMILES

Mechanism of Action

In vitro	Deoxycholic acid inhibits miR-21 expression in primary rat hepatocytes in a dose-dependent manner, and increases miR-21 pro-apoptotic target programmed cell death 4 (PDCD4) and apoptosis. This compound decreases NF-κB activity, shown to represent an upstream mechanism leading to modulation of the miR-21/PDCD4 pathway.
In vivo	miR-21 expression is down-regulated upon short exposures to Deoxycholic acid, as is the downstream pathway, with concomitant PIDD processing and activation of caspase-2, which contributes to this compound-induced liver damage.
References	[1] http://www.nature.com/articles/srep17528

Clinical Trial Information

(data from https://clinicaltrials.gov, updated on 2026-01-08)

NCT Number	Recruitment	Conditions	Sponsor/Collaborators	Start Date	Phases
NCT06755541	RECRUITING	Primary Biliary Cholangitis (PBC)	Xijing Hospital of Digestive Diseases	2024-01-30	PHASE3
NCT07296458	RECRUITING	Primary Biliary Cholangitis (PBC)	Xijing Hospital of Digestive Diseases	2025-12-15	PHASE3
NCT06842472	NOT_YET_RECRUITING	Breast Cancer	Cancer Institute and Hospital, Chinese Academy of Medical Sciences	2025-02	PHASE2
NCT07378761	NOT_YET_RECRUITING	Immune-Mediated Cholestasis	University Hospital, Montpellier	2026-12	PHASE2
NCT07717242	RECRUITING	Bladder Urothelial Carcinoma; Bladder Cancer	Sun Yat-Sen Memorial Hospital of Sun Yat-Sen University	2026-07-01	PHASE2
NCT06591468	ENROLLING_BY_INVITATION	Primary Biliary Cholangitis (PBC)	Han Ying	2024-10-30	PHASE2; PHASE3

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Handling Instructions

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