Catalpol

Catalog No.S3909 Synonyms: Catalpinoside, Digitalis purpurea L

For research use only.

Catalpol (Catalpinoside, Digitalis purpurea L), an iridoid glucoside contained richly in the roots of the small flowering plant species Rehmannia glutinosa Libosch, has been shown to have antioxidation, anti-inflammation, anti-apoptosis and other neuroprotective properties and plays a role in neuroprotection against hypoxic/ischemic injury, AD and PD in both in vivo and in vitro models.

Catalpol Chemical Structure

CAS No. 2415-24-9

Purity & Quality Control

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Biological Activity

Description Catalpol (Catalpinoside, Digitalis purpurea L), an iridoid glucoside contained richly in the roots of the small flowering plant species Rehmannia glutinosa Libosch, has been shown to have antioxidation, anti-inflammation, anti-apoptosis and other neuroprotective properties and plays a role in neuroprotection against hypoxic/ischemic injury, AD and PD in both in vivo and in vitro models.
In vitro

Catalpol is able to inhibit HCT116 cell proliferation. Furthermore, catalpol induces apoptosis in HCT116 cells, which depends on the increased activities of caspase-3 and −9. In addition, catalpol reduces the expression of PI3K, p-Akt and Akt in HCT116 cells[1]. In vitro, catalpol inhibits TNF-α, IL-6, IL-4 and IL-1β production and up-regulates IL-10 expression in LPS-stimulated alveolar macrophages[2].

In vivo Catalpol decreases peroxynitrite formation and consequently exerts cardioprotective effects through the PI3K/Akt signaling pathway in ischemic/reperfusion rats. Catalpol inhibits lung W/D ratio, myeloperoxidase activity of lung samples, the amounts of inflammatory cells and TNF-α, IL-6, IL-4 and IL-1β in BALF induced by LPS. The production of IL-10 in BALF is up-regulated by catalpol[2].

Protocol (from reference)

Cell Research:[1]
  • Cell lines: HCT116 cells
  • Concentrations: 0, 25, 50 and 100 µg/ml
  • Incubation Time: 24, 48, 72 h
  • Method: HCT116 cells (2×104 cells/well) are seeded in 96-well plates and cell viability is detected using MTT. HCT116 cells are cultured with various concentrations of catalpol (0, 25, 50 and 100 µg/ml). Following treatment for 24, 48, and 72 h, 20 µl MTT solution (0.5 mg/ml) is added into each well and cells are incubated at 37°C for 4 h. The culture medium of each well is subsequently removed and 150 µl dimethyl sulfoxide is added into each well at room temperature whilst being shaken for 20 min. Absorbance is measured at 570 nm using a Bio-Rad ELISA reader.
  • (Only for Reference)
Animal Research:[2]
  • Animal Models: Mice acute lung injury model (Male BALB/c mice)
  • Dosages: 2.5, 5 and 10 mg/kg
  • Administration: i.p.
  • (Only for Reference)

Solubility (25°C)

In vitro

DMSO 72 mg/mL
(198.71 mM)


* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 362.33
Formula

C15H22O10

CAS No. 2415-24-9
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles C1=COC(C2C1C(C3C2(O3)CO)O)OC4C(C(C(C(O4)CO)O)O)O

In vivo Formulation Calculator (Clear solution)

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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
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Molarity Calculator

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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