BSJ-4-116

Catalog No.S9859

For research use only.

BSJ-4-116 is a specific degrader of cyclin-dependent kinase 12 (CDK12). BSJ-4-116 exhibits potent antiproliferative effects.

BSJ-4-116 Chemical Structure

CAS No. 2519823-34-6

Purity & Quality Control

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Biological Activity

Description BSJ-4-116 is a specific degrader of cyclin-dependent kinase 12 (CDK12). BSJ-4-116 exhibits potent antiproliferative effects.
Targets
CDK12 [1]
In vitro

BSJ-4-116 selectively degrads CDK12 as assessed through quantitative proteomics. BSJ-4-116 downregulates the expression of DDR genes and exhibits antiproliferative activity in cancer cells. BSJ-4-116 also substantially suppresses the phosphorylation of Pol II Ser2 and Thr4.[1]

Protocol (from reference)

Cell Research:

[1]

  • Cell lines: Jurkat cells
  • Concentrations: 50 nM
  • Incubation Time: 8 h
  • Method:

    For poly(A) 3'-end sequencing, Jurkat cells are exposed to DMSO or THZ531 (250 nM) or to BSJ-4-116 or BSJ-4-116-NC (50 nM) for 8 h. RNA extraction is performed with TRIzol following the manufacturer's instructions. Total RNA is treated with DNase I. Sequencing libraries are prepared with the RNA-sequencing library kit following the manufacturer's instructions. All samples are sequenced on a HiSeq 2500 sequencer.

Solubility (25°C)

In vitro

Chemical Information

Molecular Weight 837.38
Formula

C40H49ClN8O8S

CAS No. 2519823-34-6
Storage 3 years -20°C powder
2 years -80°C in solvent

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Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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