Home Cell Cycle CDK inhibitor - PROTAC modulator BSJ-03-123 For research use only.

BSJ-03-123

Synonyms: BSJ

BSJ-03-123 is a phthalimide-based degrader of cyclin-dependent kinase 6 (CDK6). (PROTAC)

BSJ-03-123 Chemical Structure

BSJ-03-123 Chemical Structure

CAS: 2361493-16-3

Purity & Quality Control

Batch: S881501 DMSO] 100 mg/mL] false] Water] ˂1 mg/mL] false] Ethanol] ˂1 mg/mL] false Purity: 98.62%
98.62

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Biological Activity

Description BSJ-03-123 is a phthalimide-based degrader of cyclin-dependent kinase 6 (CDK6). (PROTAC)
Targets
CDK6 [1]
In vitro
In vitro

BSJ-03-123 is a phthalimide-based degrader that exploits protein-interface determinants to achieve proteome-wide selectivity for the degradation of cyclin-dependent kinase 6 (CDK6). Pharmacologic CDK6 degradation targets a selective dependency of acute myeloid leukemia cells, and transcriptomics and phosphoproteomics profiling of acute degradation of CDK6 enabled dynamic mapping of its immediate role in coordinating signaling and transcription.[1]
Cell Research Cell lines MV4–11, THP-1, MOLM13, P31/FUJ, MOLT4, NCI-H358, HT29, HEK293T and Hs578T cells
Concentrations 50 nM, 100 nM, 200 nM, 500 nM, 1000 nM
Incubation Time 2 h, 4 h, 24 h
Method

For cell cycle measurement, 106 cells per well are seeded in 24-well plates in triplicates. After 24 h of BSJ-03-123 treatment, cells are harvested by centrifugation at 500g for 5 min and washed in 1 ml cold PBS. Cells are spun down again, resuspended in 200 μl PBS and fixed by addition of 800 μl 70% EtOH and incubation for 20 min at −20°C.

Chemical Information & Solubility

Molecular Weight 937.01 Formula

C47H56N10O11
CAS No. 2361493-16-3 SDF --
Smiles CC1=C(C(=O)N(C2=NC(=NC=C12)NC3=NC=C(C=C3)N4CCN(CC4)CCOCCOCCOCCNC(=O)COC5=CC=CC6=C5C(=O)N(C6=O)C7CCC(=O)NC7=O)C8CCCC8)C(=O)C
Storage (From the date of receipt) 3 years -20°C powder

In vitro
Batch:

DMSO : 100 mg/mL ( (106.72 mM)； Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : ˂1 mg/mL

Ethanol : ˂1 mg/mL


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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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