Bromosporine

Catalog No.S7233

For research use only.

Bromosporine is a broad spectrum inhibitor for bromodomains with IC50 of 0.41 μM, 0.29 μM, 0.122 μM and 0.017 μM for BRD2, BRD4, BRD9 and CECR2, respectively.

Bromosporine Chemical Structure

CAS No. 1619994-69-2

Selleck's Bromosporine has been cited by 5 Publications

Purity & Quality Control

Choose Selective Epigenetic Reader Domain Inhibitors

Biological Activity

Description Bromosporine is a broad spectrum inhibitor for bromodomains with IC50 of 0.41 μM, 0.29 μM, 0.122 μM and 0.017 μM for BRD2, BRD4, BRD9 and CECR2, respectively.
Targets
CECR2 [1]
()
BRD9 [1]
()
BRD4 [1]
()
BRD2 [1]
()
17 nM 0.122 μM 0.29 μM 0.41 μM
In vitro

In cell-based assays, Bromosporine (1 µM) accelerates FRAP recovery of BRD4 and CREBBP, while shows no activities against TIF1α, BAZ2A, and SMARCA2 even at 10 µM. Bromosporine shows moderate cytotoxicity in HeLa cells at 18 µM. Bromosporine, as a chemical probe for bromodomain functional assays, will be very useful in elucidating further biological roles of reader domains. [2]

Protocol (from reference)

Solubility (25°C)

In vitro

DMSO 81 mg/mL
(200.27 mM)
Water Insoluble
Ethanol Insoluble

Chemical Information

Molecular Weight 404.44
Formula

C17H20N6O4S

CAS No. 1619994-69-2
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles CCOC(=O)NC1=CC(=NN2C1=NN=C2C)C3=CC(=C(C=C3)C)NS(=O)(=O)C

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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