Apostatin-1 (Apt-1)

Apostatin-1 (Apt-1) is a novel TRADD inhibitor. Apostatin-1 binds to a pocket on the N-terminal TRAF2-binding domain of TRADD (TRADD-N). Apostatin-1 inhibits bortezomib-induced apoptosis and RIPK1-dependent apoptosis (RDA) with an IC50 of about 1 μM.

Apostatin-1 (Apt-1) Chemical Structure

Apostatin-1 (Apt-1) Chemical Structure

CAS: 2559703-06-7

Purity & Quality Control

Batch: S970501 DMSO] 69 mg/mL] false] Ethanol] 9 mg/mL] false] Water] Insoluble] false Purity: 99.56%
99.56

Apostatin-1 (Apt-1) Related Products

Choose Selective TRADD Inhibitors

Biological Activity

Description Apostatin-1 (Apt-1) is a novel TRADD inhibitor. Apostatin-1 binds to a pocket on the N-terminal TRAF2-binding domain of TRADD (TRADD-N). Apostatin-1 inhibits bortezomib-induced apoptosis and RIPK1-dependent apoptosis (RDA) with an IC50 of about 1 μM.
Targets
TRADD [1]
In vitro
In vitro

Apostatin-1 (Apt-1) is a TRADD inhibitor by combining with the pocket on the N-terminal TRAF2 binding domain of TRADD (TRADD-N) to modulate the ubiquitination of RIPK1 and beclin 1. Apostatin-1 effectively inhibits bortezomib-induced apoptosis and RIPK1-dependent apoptosis (RDA) in vitro.<sup><a class="sref" href="#s_ref">[1]</a></sup>

Cell Research Cell lines Jurkat (ATCC TIB-152), MEF (CF-1) (ATCC SCRC-1040)
Concentrations 10 µM
Incubation Time 24 h for Jurkat cells; 8 h for mouse embryonic fibroblasts (MEFs)
Method

Jurkat cells were treated with Velcade (50 nM) and  Apt-1 (10 μM) for 24 h and cell viability was measured; In MEFs were treated with mTNF (1 ng/mL) and 5Z-7- Oxozeaenol (0.5 μM) to achieve RIPK1-dependent apoptosis (RDA),  added Apt-1 at different concentrations for 8 h and cell survival was measured.

Chemical Information & Solubility

Molecular Weight 345.50 Formula

C19H27N3OS

CAS No. 2559703-06-7 SDF --
Smiles CN1CCN=C1SC(C(=O)NC2CCCCCC2)C3=CC=CC=C3
Storage (From the date of receipt) 3 years -20°C powder

In vitro
Batch:

DMSO : 69 mg/mL ( (199.71 mM); Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : 9 mg/mL

Water : Insoluble


Molecular Weight Calculator

In vivo
Batch:

Add solvents to the product individually and in order.


In vivo Formulation Calculator

Preparing Stock Solutions

Molarity Calculator

Mass Concentration Volume Molecular Weight

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

mg/kg g μL

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO % % Tween 80 % ddH2O
%DMSO %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
If you have any other enquiries, please leave a message.

* Indicates a Required Field

Please enter your name.
Please enter your email. Please enter a valid email address.
Please write something to us.
Tags: buy Apostatin-1 (Apt-1) | Apostatin-1 (Apt-1) supplier | purchase Apostatin-1 (Apt-1) | Apostatin-1 (Apt-1) cost | Apostatin-1 (Apt-1) manufacturer | order Apostatin-1 (Apt-1) | Apostatin-1 (Apt-1) distributor