Ac-FLTD-CMK

Catalog No.S9817

For research use only.

Ac-FLTD-CMK is a specific inhibitor for inflammatory caspases. Ac-FLTD-CMK inhibits gasdermin D (GSDMD) cleavage by caspases-1, -4, -5, and -11 in vitro. Ac-FLTD-CMK is effective against caspases-1, caspases-4 and caspases-5 with IC50 of 46.7 nM, 1.49 μM and 329 nM, respectively.

Ac-FLTD-CMK Chemical Structure

CAS No. 2376255-48-8

Purity & Quality Control

Choose Selective Pyroptosis Inhibitors

Biological Activity

Description Ac-FLTD-CMK is a specific inhibitor for inflammatory caspases. Ac-FLTD-CMK inhibits gasdermin D (GSDMD) cleavage by caspases-1, -4, -5, and -11 in vitro. Ac-FLTD-CMK is effective against caspases-1, caspases-4 and caspases-5 with IC50 of 46.7 nM, 1.49 μM and 329 nM, respectively.
Targets
caspases-1 [1]
(Cell-free assay)
caspases-5 [1]
(Cell-free assay)
caspases-4 [1]
(Cell-free assay)
46.7 nM 329 nM 1.49 μM
In vitro

A GSDMD-derived inhibitor, N-acetyl-Phe-Leu-Thr-Asp-chloromethylketone (Ac-FLTD-CMK), inhibits GSDMD cleavage in vitro and suppresses pyroptosis downstream of both canonical and noncanonical inflammasomes.[1]

In vivo

Ac-FLTD-CMK inhibits pyroptosis and exerts neuroprotective effect in a mice model of traumatic brain injury.[2]

Protocol (from reference)

Cell Research:

[1]

  • Cell lines: BMDM cells
  • Concentrations: 10 μM
  • Incubation Time: 30 mins
  • Method:

    To observe ASC speck formation, BMDM cells expressing cerulean-ASC are plated at 2 x 104 /well in 96-well plates the day prior to experiment. Cells are primed with 200 ng/ml LPS for 4 hours before stimulating with either 5 mM ATP or 10 μM nigericin to activate the NLRP3 inflammasome, in the presence or absence of 10 μM z-VAD-FMK, Ac-FLTD-CMK, or MCC950. After 30 mins, the cerulean-ASC speck formation is observed with a Leica DMIL fluorescence microscopy.

Animal Research:

[2]

  • Animal Models: 10–12 week-old male C57BL/6 mice
  • Dosages: 0.5 μg for each mouse
  • Administration: ICV

Solubility (25°C)

In vitro

Chemical Information

Molecular Weight 569.05
Formula

C26H37ClN4O8

CAS No. 2376255-48-8
Storage 3 years -20°C powder
2 years -80°C in solvent

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

mg/kg g μL

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO % % Tween 80 % ddH2O
%DMSO %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
If you have any other enquiries, please leave a message.

* Indicates a Required Field

Please enter your name.
Please enter your email. Please enter a valid email address.
Please write something to us.
Tags: buy Ac-FLTD-CMK | Ac-FLTD-CMK supplier | purchase Ac-FLTD-CMK | Ac-FLTD-CMK cost | Ac-FLTD-CMK manufacturer | order Ac-FLTD-CMK | Ac-FLTD-CMK distributor