Ac-FLTD-CMK

For research use only.

Catalog No.S9817

Ac-FLTD-CMK Chemical Structure

CAS No. 2376255-48-8

Ac-FLTD-CMK is a specific inhibitor for inflammatory caspases. Ac-FLTD-CMK inhibits gasdermin D (GSDMD) cleavage by caspases-1, -4, -5, and -11 in vitro. Ac-FLTD-CMK is effective against caspases-1, caspases-4 and caspases-5 with IC50 of 46.7 nM, 1.49 μM and 329 nM, respectively.

Purity & Quality Control

Choose Selective Pyroptosis Inhibitors

Biological Activity

Description Ac-FLTD-CMK is a specific inhibitor for inflammatory caspases. Ac-FLTD-CMK inhibits gasdermin D (GSDMD) cleavage by caspases-1, -4, -5, and -11 in vitro. Ac-FLTD-CMK is effective against caspases-1, caspases-4 and caspases-5 with IC50 of 46.7 nM, 1.49 μM and 329 nM, respectively.
Targets
caspases-1 [1]
(Cell-free assay)
caspases-5 [1]
(Cell-free assay)
caspases-4 [1]
(Cell-free assay)
46.7 nM 329 nM 1.49 μM
In vitro

A GSDMD-derived inhibitor, N-acetyl-Phe-Leu-Thr-Asp-chloromethylketone (Ac-FLTD-CMK), inhibits GSDMD cleavage in vitro and suppresses pyroptosis downstream of both canonical and noncanonical inflammasomes.[1]

In vivo

Ac-FLTD-CMK inhibits pyroptosis and exerts neuroprotective effect in a mice model of traumatic brain injury.[2]

Protocol

Cell Research:

[1]

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  • Cell lines: BMDM cells
  • Concentrations: 10 μM
  • Incubation Time: 30 mins
  • Method:

    To observe ASC speck formation, BMDM cells expressing cerulean-ASC are plated at 2 x 104 /well in 96-well plates the day prior to experiment. Cells are primed with 200 ng/ml LPS for 4 hours before stimulating with either 5 mM ATP or 10 μM nigericin to activate the NLRP3 inflammasome, in the presence or absence of 10 μM z-VAD-FMK, Ac-FLTD-CMK, or MCC950. After 30 mins, the cerulean-ASC speck formation is observed with a Leica DMIL fluorescence microscopy.


    (Only for Reference)
Animal Research:

[2]

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  • Animal Models: 10–12 week-old male C57BL/6 mice
  • Dosages: 0.5 μg for each mouse
  • Administration: ICV
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 100 mg/mL (175.73 mM)
Water 2 mg/mL (3.51 mM)
Ethanol ''''25 mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 569.05
Formula

C26H37ClN4O8

CAS No. 2376255-48-8
Storage powder
in solvent
Synonyms N/A

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Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID