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Cyclovirobuxine D

Name: Cyclovirobuxine D
Brand: Selleck Chemicals
SKU: S2402
Availability: InStock
Rating: 5 (1 reviews)

Cat.No.S2402

Cyclovirobuxine D (Cyclovirobuxin D, Cyclovirobuxine, Bebuxine, CVB-D) is an active compound extracted from Buxus microphylla, which has been used for treating acute myocardial ischemia.

Chemical Structure

Molecular Weight: 402.66

Jump to Mechanism of Action Solubility Chemical Information, Storage & Stability

Quality Control

Batch: Purity: 99.92%

99.92

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Chemical Information, Storage & Stability

Molecular Weight	402.66	Formula	C₂₆H₄₆N₂O	Storage (From the date of receipt)	3 years-20°Cpowder
CAS No.	860-79-7	Download SDF		Storage of Stock Solutions	1 year-80°Cin solvent 1 month-20°Cin solvent
Synonyms	Cyclovirobuxin D, Cyclovirobuxine, Bebuxine, CVB-D			Smiles	CC(C1C(CC2(C1(CCC34C2CCC5C3(C4)CCC(C5(C)C)NC)C)C)O)NC

Solubility

In vitro
Batch:

Ethanol : 20 mg/mL

DMSO : Insoluble ( Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : Insoluble

Molarity Calculator

Mass	Concentration	Volume	Molecular Weight
=	×	×

Dilution Calculator Molecular Weight Calculator

In vivo Batch:
Clear solution	5%ethyl alcohol 1 40%PEG300 2 5%Tween80 3 50%ddH2O Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	1mg/ml (2.48mM)	Taking the 1 mL working solution as an example, add 50 μL of 20 mg/ml clarified ethyl alcohol stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Clear solution	5% ethyl alcohol 1 95% Corn oil Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	1mg/ml (2.48mM)	Taking the 1 mL working solution as an example, add 50 μL of 20 mg/ml clear ethyl alcohol stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg Average weight of animals: g Dosing volume per animal:μL Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO + % + % Tween 80 + % ddH₂O

%DMSO+ %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Mechanism of Action

In vitro	Cyclovirobuxine D increases cardiomyocytes viability injured by oxidation or hypoxia. It significantly reduces the infarct size induced by ligating the coronary artery in rats. In addition, this compound protects rat aorta endothelial cells against hypoxia and enhanced nitric oxide (NO) release from endothelial cells. ^[1] It facilitates the utilization of intracellular Ca(2+) and prevents the loss of Ca(2+), which may be the underlying mechanism of its protective effect on heart failure. ^[4]
In vivo	Cyclovirobuxine D decreases the weight of venous thrombus in rats. ^[1] This compound causes a primary effect of coronary vasodilatation in anesthetized pigs, which involves mechanisms related to the endothelial release of nitric oxide. ^[2] In addition, it is beneficial for heart failure in rats induced by myocardial infarction. ^[3] LD50: Mice 8.9mg/kg (i.v.), 9.2mg/kg (i.p.), 293mg/kg (i.g.). ^[5]
References	https://pubmed.ncbi.nlm.nih.gov/17555743/ https://pubmed.ncbi.nlm.nih.gov/10462084/ https://pubmed.ncbi.nlm.nih.gov/21575690/ https://pubmed.ncbi.nlm.nih.gov/23022536/

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