Ponatinib (AP24534)

Name: Ponatinib (AP24534)
Brand: Selleck Chemicals
SKU: S1490
Rating: 5 (151 reviews)

For research use only.

Catalog No.S1490

151 publications

CAS No. 943319-70-8

Ponatinib (AP24534) is a novel, potent multi-target inhibitor of Abl, PDGFRα, VEGFR2, FGFR1 and Src with IC50 of 0.37 nM, 1.1 nM, 1.5 nM, 2.2 nM and 5.4 nM in cell-free assays, respectively. Ponatinib (AP24534) inhibits autophagy.

Selleck's Ponatinib (AP24534) has been cited by 151 publications

Nature, 2020, 583(7818):845-851

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Cancer Cell, 2019, 36(2):179-193

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Cell, 2019, 36(2):179-193

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Cancer Cell, 2019, 36(4):431-443

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Nature, 2016, 30;534(7609):647-51.

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Cancer Discov, 2016, 6(7):727-39

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Nature, 2015, 10.1038/nature14329

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Cancer Cell, 2014, 26(2):207-21

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Cancer Cell, 2012, 22(5):656-67

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Acta Neuropathol, 2021, 10.1007/s00401-021-02327-x

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Mol Syst Biol, 2021, 17(9):e10426

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Cancers (Basel), 2021, 13(2)E361

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Microb Biotechnol, 2021, 10.1111/1751-7915.13814

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Mol Pharmacol, 2021, 99(6):435-447

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J Ethnopharmacol, 2021, S0378-8741(21)00896-5

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Eur J Pharmacol, 2021, 910:174446

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Ann Hematol, 2021, 10.1007/s00277-020-04357-z

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Ticks Tick Borne Dis, 2021, 12(2):101611

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Oncol Lett, 2021, 21(4):294

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Hum Cell, 2021, 10.1007/s13577-021-00579-z

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Rapid Commun Mass Spectrom, 2021, e9130

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Vet J, 2021, 269:105621

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J Dermatol Sci, 2021, S0923-1811(21)00005-0

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Eur J Pharmacol, 2021, 895:173868

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Nat Commun, 2020, 11(1):5704

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Nat Commun, 2020, 11(1):4370

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Int J Cancer, 2020, 10.1002/ijc.32924

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Cell Death Dis, 2020, 11(10):884

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FASEB J, 2020, 34(3):3773-3791

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Mol Cell Proteomics, 2020, 10.1074/mcp.RA119.001504

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J Infect Dis, 2020, 3;jiaa295

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Dis Model Mech, 2020, dmm.047779

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Cancer Cell Int, 2020, 19;20:58

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Front Pharmacol, 2020, 30;11:543

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BMC Cancer, 2020, 20(1):592

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Oncol Rep, 2020, 44(6):2581-2594

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Hum Cell, 2020, 10.1007/s13577-020-00420-z

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Hum Cell, 2020, 10.1007/s13577-020-00425-8

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Exp Lung Res, 2020, 1-17

PubMed: 33107354 ( click the link to review the publication )

Nature, 2020, 583(7818):845-851

PubMed: 32699415 ( click the link to review the publication )

Biochim Biophys Acta Gen Subj, 2020, 1864(4):129507

PubMed: 31881245 ( click the link to review the publication )

Cancer Chemother Pharmacol, 2020, 10.1007/s00280-019-04022-x

PubMed: 31901955 ( click the link to review the publication )

Commun Biol, 2020, 3(1):18

PubMed: 31925328 ( click the link to review the publication )

Front Cell Dev Biol, 2020, 8:408

PubMed: 32596239 ( click the link to review the publication )

Cold Spring Harb Mol Case Stud, 2020, 1;6(2) pii: a004838

PubMed: 31980503 ( click the link to review the publication )

Nat Cell Biol, 2019, 21(6):778-790

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Nat Commun, 2019, 10(1):2532

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Nat Commun, 2019, 10(1):2701

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Clin Cancer Res, 2019, 10.1158/1078-0432.CCR-18-2779

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Cell Rep, 2019, 26(1):65-78

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Cell Rep, 2019, 28(9):2331-2344

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Protein Cell, 2019, 10(3):161-177

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Br J Cancer, 2019, 120(6):612-620

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Mol Cancer Ther, 2019, 18(11):1985-1996

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FEBS J, 2019, 286(22):4443-4472

PubMed: 31155838 ( click the link to review the publication )

Front Oncol, 2019, 9:103

PubMed: 30931252 ( click the link to review the publication )

J Proteome Res, 2019, 18(1):522-534

PubMed: 30540191 ( click the link to review the publication )

Cell Cycle, 2019, 18(18):2307-2322

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Transl Oncol, 2019, 12(9):1221-1228

PubMed: 31276961 ( click the link to review the publication )

PLoS One, 2019, 14(11):e0217451

PubMed: 31682640 ( click the link to review the publication )

Leuk Lymphoma, 2019, 60(1):189-199

PubMed: 29741440 ( click the link to review the publication )

Exp Dermatol, 2019, 10.1111/exd.14010

PubMed: 31338879 ( click the link to review the publication )

Exp Hematol, 2019, 77:36-40.e2

PubMed: 31493432 ( click the link to review the publication )

Clin Exp Pharmacol Physiol, 2019, 46(2):126-136

PubMed: 30251267 ( click the link to review the publication )

Leukemia, 2019, 33(8):1964-1977

PubMed: 30842608 ( click the link to review the publication )

Hemasphere, 2019, 3(2):e182

PubMed: 31723821 ( click the link to review the publication )

Cell Mol Gastroenterol Hepatol, 2019, 8(1):37-60

PubMed: 30836148 ( click the link to review the publication )

Clin Cancer Res, 2018, 24(8):1932-1943

PubMed: 29440177 ( click the link to review the publication )

Cancers (Basel), 2018, 10(12)

PubMed: 30572654 ( click the link to review the publication )

Pigment Cell Melanoma Res, 2018, 31(2):253-266

PubMed: 28972303 ( click the link to review the publication )

Mol Cancer Ther, 2018, 17(7):1405-1415

PubMed: 29695637 ( click the link to review the publication )

Br J Haematol, 2018, 181(5):642-652

PubMed: 29675955 ( click the link to review the publication )
Pharmacol Res, 2018, 134:166-178

PubMed: 29944980 ( click the link to review the publication )

J Cell Mol Med, 2018, 22(11):5596-5606

PubMed: 30160357 ( click the link to review the publication )

Transl Oncol, 2018, 11(6):1406-1418

PubMed: 30219696 ( click the link to review the publication )

Hum Gene Ther Methods, 2018, 29(1):30-43

PubMed: 29281903 ( click the link to review the publication )

Oncotarget, 2018, 9(62):31958-31970

PubMed: 30174789 ( click the link to review the publication )

Oncotarget, 2018, 9(41):26491-26506

PubMed: 29899872 ( click the link to review the publication )

Oncotarget, 2018, 9(78):34735-34747

PubMed: 30410673 ( click the link to review the publication )

Respir Res, 2018, 19(1):120

PubMed: 29921306 ( click the link to review the publication )

Oncotarget, 2018, 9(100):37352-37366

PubMed: 30647837 ( click the link to review the publication )

Oncotarget, 2018, 9(65):32496-32506

PubMed: 30197758 ( click the link to review the publication )

Sci Adv, 2018, 4(11):eaau0731

PubMed: 30417093 ( click the link to review the publication )

Sci Transl Med, 2017, 14;9(394):eaah6144

PubMed: 28615362 ( click the link to review the publication )

Elife, 2017, 6

PubMed: 28199182 ( click the link to review the publication )

J Exp Clin Cancer Res, 2017, 36(1):8

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Arch Toxicol, 2017, 91(8):2921-2938

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Mol Cancer Ther, 2017, 10.1158/1535-7163.MCT-17-0008

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Mol Cancer Ther, 2017, 16(10-:2130-2143

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Mol Cancer Ther, 2017, 16(9):1729-1738

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Hum Mol Genet, 2017, 26(10):1801-1810

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Sci Rep, 2017, 7(1-:5519

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ChemMedChem, 2017, 12(16):1390-1398

PubMed: 28639308 ( click the link to review the publication )

Leuk Lymphoma, 2017, 58(6):1455-1467

PubMed: 27733071 ( click the link to review the publication )

SLAS Discov, 2017, 22(7):915-922

PubMed: 28346107 ( click the link to review the publication )

SLAS Discov, 2017, 22(5):507-515

PubMed: 28324660 ( click the link to review the publication )
Oncotarget, 2017, 8(16):26613-26624

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Oncotarget, 2017, 8(40):67709-67722

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Sci Rep, 2017, 7(1):15219

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Oncotarget, 2017, 8(65):109319-109331

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Oncotarget, 2017, 8(19):31666-31681

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Gut, 2016, 10.1136/gutjnl-2015-310382

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Blood, 2016, 28;127(17):2131-43

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Hepatology, 2016, 64(2):488-500

PubMed: 27015352 ( click the link to review the publication )

J Thorac Oncol, 2016, 11(8):1273-1281

PubMed: 27068398 ( click the link to review the publication )

Leukemia, 2016, 30(7):1493-501

PubMed: 27044711 ( click the link to review the publication )

Oncogene, 2016, 35(25):3239-48

PubMed: 26455322 ( click the link to review the publication )

Mol Cell Proteomics, 2016, 15(3):1139-50

PubMed: 26933192 ( click the link to review the publication )

Cancer Sci, 2016, 107(2):140-8

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Mol Pharm, 2016, 13(11):3724-3735

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Sci Rep, 2016, 6:29559

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Oncotarget, 2016, 7(47):78083-78094

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Tumour Biol, 2016, 37(2):2365-78

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Genes Chromosomes Cancer, 2016, 55(1):60-8

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Oncotarget, 2016, 7(31-:50161-50179

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Oncotarget, 2016, 7(12):13886-901

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Sci Transl Med, 2015, 7(298):298ra119

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Nat Commun, 2015, 6:7002

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Leukemia, 2015, 29(5)

PubMed: 25394714 ( click the link to review the publication )

Leukemia, 2015, 29(12):2390-2

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Cancer Lett, 2015, 365(2):174-81

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J Med Chem, 2015, 58(23):9228-37

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Cell Death Dis, 2015, 6:e1767

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Toxicol Sci, 2015, 143(1):147-55

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PLoS One, 2015, 10(3):e0120841

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Pathol Oncol Res, 2015, 10.1007/s12253-015-9916-9

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Nat Cell Biol, 2014, 16(2):133-44

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Sci Transl Med, 2014, 6(261):261ra152

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Blood, 2014, 123(21):3296-304

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Genome Biol, 2014, 15(8):428

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Clin Cancer Res, 2014, 20(8):2092-103

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Proc Natl Acad Sci USA, 2014, 10.1073/pnas.1321173111

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Cancer Discov, 2014, 4(10):1168-81

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Mol Cancer Ther, 2014, 13(10):2315-27

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Structure, 2014, 22(12):1764-74

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Oncol Rep, 2014, 31(4):1605-12

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Thrombo Res, 2014, 135(1):155-60

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Biochem Biophys Res Commun, 2014, 446(1):54-60

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J Thorac Oncol, 2014, 9(9):1316-23

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Harvard University, 2014, Rachel Grace Liao

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Assay Drug Dev Technol, 2014, 12(9-10):514-26

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Clin Cancer Res, 2013, 19(6):1422-32

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Cancer Res, 2013, 73(16):5195-205

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Neoplasia, 2013, 15(8):975-88

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Curr Cancer Drug Targets, 2012, 12(5):505-21

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Exp Hematol, 2012, 41(4):335-45.e3

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Oncotarget, 2012, 3(12):1557-65

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Mol Genet Metab, 2010, 100(4):309-15

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Purity & Quality Control

Choose Selective Bcr-Abl Inhibitors

Biological Activity

Description

Targets

Abl ^[1] (Cell-free assay)	PDGFRα ^[1] (Cell-free assay)	VEGFR2 ^[1] (Cell-free assay)	FGFR1 ^[1] (Cell-free assay)	c-Src ^[1] (Cell-free assay)	View More
0.37 nM	1.1 nM	1.5 nM	2.2 nM	5.4 nM	View More

In vitro

AP24534 potently inhibits native Abl, Abl^T315I, and other clinically important Abl kinase domain mutants with IC50 of 0.30 nM–2 nM. AP24534 doesn't inhibit Aurora kinase family members, nor does it inhibit insulin receptor or CDK2/cyclin E. AP24534 inhibits proliferation of Ba/F3 cells expressing Bcr-Abl with IC50 of 0.5 nM, as well as Ba/F3 cells expressing a range of Bcr-Abl mutants with IC50 of 0.5 nM–36 nM. The inhibition of proliferation by AP24534 is correlated with induction of apoptosis. ^[1-2] In leukemic cell lines containing activated forms of FLT3, KIT, FGFR1, and PDGFRα receptors, AP24534 potently inhibits receptor phosphorylation and cellular proliferation with IC50 of 0.3 nM to 20 nM. In MV4-11 (FLT3-ITD(+/+)) but not RS4;11 (FLT3-ITD(–/–)) AML cells, AP24534 inhibits FLT3 signaling and induced apoptosis at less than 10 nM. AP24534 inhibits viability of primary leukemic blasts from a FLT3-ITD positive AML patient with IC50 of 4 nM but not those from patients with AML expressing native FLT3. ^[3] In Ba/F3 cells engineered to express activated FGFR1-4, AP24534 potently inhibits FGFR-mediated signaling and viability with IC50 lower than 40 nM. In cell lines representing multiple tumor types (endometrial, bladder, gastric, breast, lung, and colon), and containing FGFRs dysregulated by a variety of mechanisms, AP24534 inhibits FGFR-mediated signaling with IC50 less than 40 nM and inhibits cell growth with IC50 of 7 nM–181 nM. ^[4]

Cell Data

Cell Lines	Assay Type	Activity Description	PMID
EoL-1-cell	MojiS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	NVztNIZrUUN3ME2wMlAxODByMECzOVkh\|ryP	MXK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNVcyQDN5Lze+V2FPT0WUPD;hQi=>
MV-4-11	NVLUNVR1T3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	MVnJR\|UxRTBwMECwNFk2PiEQvF2=	M1;qe\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFEyPzF6M{evK\|5USU6JRWK8M4E,
MEG-01	NV\Ud\|JKT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	NHSxOXdKSzVyPUCuNFAxOTJ6IN88US=>	NHHkUoE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyOTdzOEO3M{c,W0GQR1XSQE9iRg>?
LAMA-84	MkfCS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	MVPJR\|UxRTBwMECwNVY2KM7:TR?=	NUXtcXdURGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTF5MUizO{8oRlODTlfFVlww[T5?
CGTH-W-1	M1PMb2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7	MULJR\|UxRTBwMECxOlkh\|ryP	NVniVHM{RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTF5MUizO{8oRlODTlfFVlww[T5?
KASUMI-1	M2TRT2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7	NHrpbXhKSzVyPUCuNFAzOTdizszN	NGOyemM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyOTdzOEO3M{c,W0GQR1XSQE9iRg>?
BE-13	MXrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	MnO4TWM2OD1yLkCwNlU6KM7:TR?=	NIXPUWg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyOTdzOEO3M{c,W0GQR1XSQE9iRg>?
CTV-1	NFT6UXRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	MkT5TWM2OD1yLkCwOVIyKM7:TR?=	NVHITGZERGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTF5MUizO{8oRlODTlfFVlww[T5?
NKM-1	NGfiRZVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	NYfFOVA2UUN3ME2wMlAxPjV3IN88US=>	M3H6TlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFEyPzF6M{evK\|5USU6JRWK8M4E,
MONO-MAC-6	M{XSNWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7	NXe5R21NUUN3ME2wMlAxQDN5IN88US=>	M1jXOlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFEyPzF6M{evK\|5USU6JRWK8M4E,
A204	NW\hXVF2T3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	M{TweWlEPTB;MD6wNVIyQCEQvF2=	MYS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNVcyQDN5Lze+V2FPT0WUPD;hQi=>
GDM-1	NIXWVnZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	M2D3SGlEPTB;MD6wNVg{OyEQvF2=	M2HJelxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFEyPzF6M{evK\|5USU6JRWK8M4E,
AN3-CA	M3TtUWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7	NGPIdZlKSzVyPUCuNFE5PzNizszN	NIjnN489[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyOTdzOEO3M{c,W0GQR1XSQE9iRg>?
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IA-LM	M1HCPGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7	MX\JR\|UxRTFwNEC5NVQh\|ryP	M{\mZVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFEyPzF6M{evK\|5USU6JRWK8M4E,
MN-60	NH6xSYtIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	M3nyVmlEPTB;MT60N\|EyPCEQvF2=	M2fxUFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFEyPzF6M{evK\|5USU6JRWK8M4E,
SIMA	MnW0S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	MVXJR\|UxRTFwNEi2PFch\|ryP	NWjT[oV{RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTF5MUizO{8oRlODTlfFVlww[T5?
NCI-H1770	M1q4fGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7	M2rWV2lEPTB;MT61NlA2PCEQvF2=	MVu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNVcyQDN5Lze+V2FPT0WUPD;hQi=>
ONS-76	M3zUPWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7	NYjNcppwUUN3ME2xMlUzOzFzIN88US=>	NEXsXXI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyOTdzOEO3M{c,W0GQR1XSQE9iRg>?
LB771-HNC	MWXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	Ml7iTWM2OD1zLkWzPVA3KM7:TR?=	MnPxQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVE4OTh\|Nz:nQnNCVkeHUkyvZV4>
SW48	NInpTpBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	MVzJR\|UxRTFwNUSyOFMh\|ryP	MUe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNVcyQDN5Lze+V2FPT0WUPD;hQi=>
KYSE-140	MWjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	MVzJR\|UxRTFwNUW3OFEh\|ryP	NWLtW5JuRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTF5MUizO{8oRlODTlfFVlww[T5?
SW626	MkXHS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	NW\jfWR3UUN3ME2xMlU5OTZizszN	MWq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNVcyQDN5Lze+V2FPT0WUPD;hQi=>
MC-CAR	MonkS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	MVLJR\|UxRTFwNUi2OlMh\|ryP	NX7TcYoyRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTF5MUizO{8oRlODTlfFVlww[T5?
D-336MG	M2D0VWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7	NGHK[4dKSzVyPUGuOlI6PCEQvF2=	NFf2RnM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyOTdzOEO3M{c,W0GQR1XSQE9iRg>?
SK-MEL-3	NYXFWoY3T3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	M3\h[2lEPTB;MT62N\|kxOiEQvF2=	NYjtRWFJRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTF5MUizO{8oRlODTlfFVlww[T5?
MHH-PREB-1	MoeyS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	MnfBTWM2OD1zLk[0OFc{KM7:TR?=	NGDxWFk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyOTdzOEO3M{c,W0GQR1XSQE9iRg>?
Calu-1	NUW2ZZJlT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	MkfYTWM2OD1zLk[1N\|g3KM7:TR?=	NIezboE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyOTdzOEO3M{c,W0GQR1XSQE9iRg>?
HCC1806	MWjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	M1;0WWlEPTB;MT62OlYyOSEQvF2=	MoPPQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVE4OTh\|Nz:nQnNCVkeHUkyvZV4>
A101D	NGG5N4lIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	MWDJR\|UxRTFwNk[3PFEh\|ryP	M4HxPFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFEyPzF6M{evK\|5USU6JRWK8M4E,
A704	M3:x[Gdzd3e2aDDJcohq[mm2aX;uJGF{e2G7	MlO1TWM2OD1zLk[4N\|EyKM7:TR?=	MVS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNVcyQDN5Lze+V2FPT0WUPD;hQi=>
LB831-BLC	NWG0SZdsT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	MUnJR\|UxRTFwNkm4OVQh\|ryP	NX7HZ2FFRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTF5MUizO{8oRlODTlfFVlww[T5?
RL95-2	MVrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	NXvaR3k6UUN3ME2xMlcyPDZ\|IN88US=>	MXK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNVcyQDN5Lze+V2FPT0WUPD;hQi=>
RPMI-2650	NFHPUGxIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	Ml7UTWM2OD1zLkeyO\|E6KM7:TR?=	M1XWVlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFEyPzF6M{evK\|5USU6JRWK8M4E,
NH-12	NXXLblQ1T3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	NV3ObFBjUUN3ME2xMlc{PDJ5IN88US=>	MmTkQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVE4OTh\|Nz:nQnNCVkeHUkyvZV4>
AGS	NETxeINIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	NWTUcFN4UUN3ME2xMlc3OzV6IN88US=>	NILuTpI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyOTdzOEO3M{c,W0GQR1XSQE9iRg>?
NEC8	NHzWRldIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	MYXJR\|UxRTFwN{i5PVUh\|ryP	NET6d2g9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyOTdzOEO3M{c,W0GQR1XSQE9iRg>?
NCI-H2009	NEfUTJFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	NIDDSXhKSzVyPUGuO\|k4OjFizszN	MY[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNVcyQDN5Lze+V2FPT0WUPD;hQi=>
A498	MVTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	NH\EeoxKSzVyPUGuPFEzQDJizszN	NETKOIo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyOTdzOEO3M{c,W0GQR1XSQE9iRg>?
NCI-H810	NIjvNWVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	M3jXR2lEPTB;MT64NVYxPiEQvF2=	NXrCb\|FWRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTF5MUizO{8oRlODTlfFVlww[T5?
SK-NEP-1	MlfHS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	MkHlTWM2OD1zLkizO\|Y4KM7:TR?=	M4rzNFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFEyPzF6M{evK\|5USU6JRWK8M4E,
D-542MG	MkfvS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	MkLSTWM2OD1zLki1PFUyKM7:TR?=	M2juRlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFEyPzF6M{evK\|5USU6JRWK8M4E,
KNS-42	MkHKS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	MYTJR\|UxRTFwOE[yOlYh\|ryP	MkDHQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVE4OTh\|Nz:nQnNCVkeHUkyvZV4>
NCI-H2452	NEH5NodIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	MVrJR\|UxRTFwOEmwO\|Qh\|ryP	MV68ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNVcyQDN5Lze+V2FPT0WUPD;hQi=>
SK-MEL-2	NXfaboYxT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	MXLJR\|UxRTFwOUCyO\|Eh\|ryP	M4TUflxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFEyPzF6M{evK\|5USU6JRWK8M4E,
OVCAR-5	M3PnN2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7	M3vmSWlEPTB;MT65NFY4PSEQvF2=	NF3TTYw9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyOTdzOEO3M{c,W0GQR1XSQE9iRg>?
SNG-M	Ml\jS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	MmDKTWM2OD1zLkm1PFU5KM7:TR?=	MmPNQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVE4OTh\|Nz:nQnNCVkeHUkyvZV4>
A673	MkOzS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	MnL1TWM2OD1zLkm3OVE3KM7:TR?=	NF\hZXo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyOTdzOEO3M{c,W0GQR1XSQE9iRg>?
L-428	NV;Qc2RYT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	M2L0O2lEPTB;MT65PVE4QSEQvF2=	Mkj5QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVE4OTh\|Nz:nQnNCVkeHUkyvZV4>
KM-H2	M2rTWGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7	NFW2N5FKSzVyPUKuNFExQSEQvF2=	M3HHUlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFEyPzF6M{evK\|5USU6JRWK8M4E,
MC-IXC	MkjPS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	MlP3TWM2OD1{LkCyNlc{KM7:TR?=	MY[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNVcyQDN5Lze+V2FPT0WUPD;hQi=>
COLO-678	NVr5TlY3T3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	M3rmdmlEPTB;Mj6wOFIxPyEQvF2=	NUS2RYpPRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTF5MUizO{8oRlODTlfFVlww[T5?
MEL-JUSO	MoXwS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	M3HLd2lEPTB;Mj6wO\|g2OSEQvF2=	M1m5NlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFEyPzF6M{evK\|5USU6JRWK8M4E,
HCC38	MkPxS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	M2DETGlEPTB;Mj6wPFQ{OyEQvF2=	NGXGfYs9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyOTdzOEO3M{c,W0GQR1XSQE9iRg>?
UACC-62	MXvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	Mn3OTWM2OD1{LkC4PFU2KM7:TR?=	MXS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNVcyQDN5Lze+V2FPT0WUPD;hQi=>
OAW-42	NX7QcppsT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	NGfiTpFKSzVyPUKuNFk4OTFizszN	MkjHQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVE4OTh\|Nz:nQnNCVkeHUkyvZV4>
LB996-RCC	M1;WOGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7	MVXJR\|UxRTJwMUO4NVch\|ryP	NYLGfXB2RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTF5MUizO{8oRlODTlfFVlww[T5?
COR-L105	M{DxdWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7	NYXM[2ZZUUN3ME2yMlE3Ozd\|IN88US=>	MnnUQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVE4OTh\|Nz:nQnNCVkeHUkyvZV4>
RCC10RGB	MXnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	MkHGTWM2OD1{LkG4OVc3KM7:TR?=	MmXnQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVE4OTh\|Nz:nQnNCVkeHUkyvZV4>
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NCI-H292	NGLmc\|RIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	M{HNPGlEPTB;Mj6yN\|k2OSEQvF2=	NEPlNIY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyOTdzOEO3M{c,W0GQR1XSQE9iRg>?
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SK-MEL-30	Mln0S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	MWLJR\|UxRTNwNkW5NlEh\|ryP	NUPEbI1ORGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTF5MUizO{8oRlODTlfFVlww[T5?
LB2241-RCC	NGOxPJFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	MmPJTWM2OD1\|Lk[2NlYh\|ryP	M3WzZlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFEyPzF6M{evK\|5USU6JRWK8M4E,
PC-14	MoXmS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	M4TWUWlEPTB;Mz62OlQ{PyEQvF2=	NFj1fYQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyOTdzOEO3M{c,W0GQR1XSQE9iRg>?
EW-7	MnfCS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	MlvVTWM2OD1\|Lk[3Olk{KM7:TR?=	NHLaOo89[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyOTdzOEO3M{c,W0GQR1XSQE9iRg>?
NMC-G1	M4DMfGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7	NG[xN3FKSzVyPUOuO\|E5OzdizszN	MXG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNVcyQDN5Lze+V2FPT0WUPD;hQi=>
TE-441-T	MV;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	MXTJR\|UxRTNwN{OwNlgh\|ryP	MnHGQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVE4OTh\|Nz:nQnNCVkeHUkyvZV4>
NCI-H1618	NUTVT25ZT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	NGH1XZZKSzVyPUOuPFE5PzVizszN	NIPac4U9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyOTdzOEO3M{c,W0GQR1XSQE9iRg>?
PANC-03-27	MWfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	MkDwTWM2OD1\|LkixPVE3KM7:TR?=	Mo\LQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVE4OTh\|Nz:nQnNCVkeHUkyvZV4>
NCI-H2228	NUfMS2FRT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	MknLTWM2OD1\|LkizNVI3KM7:TR?=	NIPsbo09[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyOTdzOEO3M{c,W0GQR1XSQE9iRg>?
CAPAN-1	MXvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	NEK2Z2tKSzVyPUOuPFcxODlizszN	NWf0d5BmRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTF5MUizO{8oRlODTlfFVlww[T5?
KLE	M3jJdGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7	NVXVd3EyUUN3ME2zMlkxPjdizszN	Mmq3QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVE4OTh\|Nz:nQnNCVkeHUkyvZV4>
Becker	NIPhT4xIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	NUH1dW5kUUN3ME2zMlkzQDR7IN88US=>	M4f4flxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFEyPzF6M{evK\|5USU6JRWK8M4E,
SK-PN-DW	NW[2SppFT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	NEfCfHFKSzVyPUSuNFc6PzRizszN	M4Pq[VxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFEyPzF6M{evK\|5USU6JRWK8M4E,
Hs-578-T	M12zW2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7	NEXwRZJKSzVyPUSuNFg{ODZizszN	M3e3WVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFEyPzF6M{evK\|5USU6JRWK8M4E,
RS4-11	M{Hxemdzd3e2aDDJcohq[mm2aX;uJGF{e2G7	NI\EPVdKSzVyPUSuNVEzPTVizszN	NFjQfJQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyOTdzOEO3M{c,W0GQR1XSQE9iRg>?
D-502MG	MUfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	Ml\0TWM2OD12LkG2PVk4KM7:TR?=	NWryd4pVRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTF5MUizO{8oRlODTlfFVlww[T5?
GI-1	M{W5Zmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7	NUXVVppDUUN3ME20MlI5Ojl7IN88US=>	NXW3WHR[RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTF5MUizO{8oRlODTlfFVlww[T5?
NCI-H209	MnfKS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	NVi2ZmpTUUN3ME20MlM{PzhizszN	MVm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNVcyQDN5Lze+V2FPT0WUPD;hQi=>
SK-OV-3	M{TpOGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7	M4OxVWlEPTB;ND60PFE1QSEQvF2=	M1\JV\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFEyPzF6M{evK\|5USU6JRWK8M4E,
LOUCY	MkCxS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	MVTJR\|UxRTRwNEi0N\|Ih\|ryP	MnraQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVE4OTh\|Nz:nQnNCVkeHUkyvZV4>
NCI-H69	Mn\2S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	MVvJR\|UxRTRwNUG3PVMh\|ryP	MnrrQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVE4OTh\|Nz:nQnNCVkeHUkyvZV4>
SCC-9	MkTIS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	MmiwTWM2OD12LkWzNVczKM7:TR?=	NFvRTnU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyOTdzOEO3M{c,W0GQR1XSQE9iRg>?
NCI-H526	MWHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	MVnJR\|UxRTRwNUmyPVMh\|ryP	MUe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNVcyQDN5Lze+V2FPT0WUPD;hQi=>
NCI-H2170	NUHpVGJuT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	NFTGNZZKSzVyPUSuOlY4PzFizszN	Mm\uQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVE4OTh\|Nz:nQnNCVkeHUkyvZV4>
SR	NI\WVnNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	M1nZO2lEPTB;ND63NFA1QCEQvF2=	MWG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNVcyQDN5Lze+V2FPT0WUPD;hQi=>
CMK	NIrnPVVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	M2LiVGlEPTB;ND63NlExOSEQvF2=	MWW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNVcyQDN5Lze+V2FPT0WUPD;hQi=>
COLO-829	MYHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	MnzzTWM2OD12Lke3NFQ{KM7:TR?=	NHTLbIQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyOTdzOEO3M{c,W0GQR1XSQE9iRg>?
HuO-3N1	MUDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	NYLHVHpYUUN3ME20Mlg{OjhzIN88US=>	NWf2[GF{RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTF5MUizO{8oRlODTlfFVlww[T5?
5637	NVzhOIZvT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	MVPJR\|UxRTRwOUC0PFch\|ryP	NUHMcFlIRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTF5MUizO{8oRlODTlfFVlww[T5?
A253	MnvrS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	MoTJTWM2OD12Lkm3O\|A5KM7:TR?=	MV:8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNVcyQDN5Lze+V2FPT0WUPD;hQi=>
NB10	NEH2W2xIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	NGmy[2pKSzVyPUWuNFc1QTdizszN	M1r1UFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFEyPzF6M{evK\|5USU6JRWK8M4E,
SF539	M3vCR2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7	MlX2TWM2OD13LkC5PVU6KM7:TR?=	NWDlTZdGRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTF5MUizO{8oRlODTlfFVlww[T5?
HAL-01	NV7CXJI6T3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	NWG2eoFTUUN3ME21MlE1PjR3IN88US=>	MWW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNVcyQDN5Lze+V2FPT0WUPD;hQi=>
MHH-ES-1	M1zNWmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7	M4DTOGlEPTB;NT6xOlkzPSEQvF2=	M{XCNFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFEyPzF6M{evK\|5USU6JRWK8M4E,
DEL	MorOS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	M2DMWGlEPTB;NT6yO\|U5OiEQvF2=	MX28ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNVcyQDN5Lze+V2FPT0WUPD;hQi=>
NCI-H1299	M4jtN2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7	MnraTWM2OD13LkO1NFA4KM7:TR?=	NG\Tblg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyOTdzOEO3M{c,W0GQR1XSQE9iRg>?
ChaGo-K-1	NYW0U2JJT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	M1;kRWlEPTB;NT6zOVExPyEQvF2=	M4LFUVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFEyPzF6M{evK\|5USU6JRWK8M4E,
HMV-II	Ml\US5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	MkH3TWM2OD13LkO5OFMh\|ryP	NX3IRVFvRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTF5MUizO{8oRlODTlfFVlww[T5?
NB69	MYnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	NFiyWWpKSzVyPUWuN\|k3OzFizszN	NYe1dWRSRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTF5MUizO{8oRlODTlfFVlww[T5?
NCI-H1882	NHiwfJRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	MUnJR\|UxRTVwNEOzOlQh\|ryP	M{e4NlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFEyPzF6M{evK\|5USU6JRWK8M4E,
SNU-475	MVzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	MWDJR\|UxRTVwNEexO\|Eh\|ryP	NWfLOZlLRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTF5MUizO{8oRlODTlfFVlww[T5?
RXF393	MWjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	MXLJR\|UxRTVwNEi0PVQh\|ryP	MXS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNVcyQDN5Lze+V2FPT0WUPD;hQi=>
CAL-51	MUDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	M{nDUmlEPTB;NT62Nlg2OyEQvF2=	MoDUQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVE4OTh\|Nz:nQnNCVkeHUkyvZV4>
SW1573	MlXXS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	NXjYfmRtUUN3ME21MlY{PTN2IN88US=>	M3XseVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFEyPzF6M{evK\|5USU6JRWK8M4E,
GCT	M17hRWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7	MmnQTWM2OD13Lke1PVI6KM7:TR?=	NYfHS3ZuRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTF5MUizO{8oRlODTlfFVlww[T5?
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NCI-H510A	NUXvOmRjT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	MUTJR\|UxRTR2LkewPEDPxE1?	M1juRlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFEyPzF6M{evK\|5USU6JRWK8M4E,
LB2518-MEL	MnnnS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	MkHiTWM2OD12ND63PVM4KM7:TR?=	MVO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNVcyQDN5Lze+V2FPT0WUPD;hQi=>
TE-5	NV;ucoJnT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	MU\JR\|UxRTR3Lkm1PFEh\|ryP	MonrQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVE4OTh\|Nz:nQnNCVkeHUkyvZV4>
EW-24	MnfkS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	NYnvVWFPUUN3ME20Ok4yPzJ\|IN88US=>	NECwS\|Y9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyOTdzOEO3M{c,W0GQR1XSQE9iRg>?
LB647-SCLC	NUDtVWVkT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	Ml61TWM2OD12Nj6yNVU2KM7:TR?=	MVq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNVcyQDN5Lze+V2FPT0WUPD;hQi=>
NCI-H524	MYnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	M4LRRWlEPTB;NE[uO\|E5PSEQvF2=	MkHXQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVE4OTh\|Nz:nQnNCVkeHUkyvZV4>
EW-11	NH\tWI1Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	NIPac4dKSzVyPUS2Mlk{PzNizszN	NV7sVJY2RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTF5MUizO{8oRlODTlfFVlww[T5?
KALS-1	NWe0Z\|NGT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	Mke3TWM2OD12Nz6yNFY3KM7:TR?=	MYK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNVcyQDN5Lze+V2FPT0WUPD;hQi=>
BT-474	MlfYS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	M3XUU2lEPTB;NEeuOFIyPiEQvF2=	NX3uV2FWRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTF5MUizO{8oRlODTlfFVlww[T5?
SK-N-AS	MVrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	NEnyRZZKSzVyPUS3Mlg5QCEQvF2=	M3PHSlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFEyPzF6M{evK\|5USU6JRWK8M4E,
HCC2157	NI[2SnNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	M1\NcGlEPTB;NEiuNFc3KM7:TR?=	NX[3dWpxRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTF5MUizO{8oRlODTlfFVlww[T5?
HCC1954	M4HrZ2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7	MYHJR\|UxRTR6LkG3NFQh\|ryP	NH3Td489[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyOTdzOEO3M{c,W0GQR1XSQE9iRg>?
FADU	NHXDO4ZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	MXjJR\|UxRTR6LkOwN\|Eh\|ryP	NYPuWGNCRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTF5MUizO{8oRlODTlfFVlww[T5?
S-117	MnruS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	NFXYclhKSzVyPUS4MlM5OTVizszN	MYC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNVcyQDN5Lze+V2FPT0WUPD;hQi=>
LS-1034	M2LsW2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7	NITM[VlKSzVyPUS4MlcxPjdizszN	M4G2NlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFEyPzF6M{evK\|5USU6JRWK8M4E,
CAMA-1	NV;s[ZZbT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	M2TOWWlEPTB;NEmuOVU1OyEQvF2=	MkH0QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVE4OTh\|Nz:nQnNCVkeHUkyvZV4>
LU-134-A	M{K4Vmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7	MmW4TWM2OD12OT64NFg2KM7:TR?=	NVP2e2VxRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTF5MUizO{8oRlODTlfFVlww[T5?
NCI-H1838	NXvOPYtIT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	NG[xemFKSzVyPUS5MlgxQDdizszN	NGXlWHg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyOTdzOEO3M{c,W0GQR1XSQE9iRg>?

... Click to View More Cell Line Experimental Data

Assay

Methods	Test Index	PMID
Western blot	pSrc / Src / pPlcg / Plcg / pStat5 / Stat5 ; PubMed: 22781593 Leukemia In (B) inhibition of Src, Plcg and Stat5 was seen in MLNAF cell lines treated with Ponatinib; ZNF112 expresses ZMYM2–FGFR1, CEP2A and CEP5A cells express CEP110–FGFR1 and BBC2 expresses BCR–FGFR1. pFGFR1(pY20) / FGFR1; PubMed: 22781593 Leukemia Western blot analysis, using various antibodies specific for downstream targets of FGFR1, demonstrates that Ponatinib inhibits the activation of Plcg and Src as a result of inhibiting the FGFR1 fusion kinase in human KG1 cells. cleaved caspase-3 / cleaved caspase-7 / BAX / Bcl-2; PubMed: 30959969 Molecules Western blot analysis of cleaved caspase-3, cleaved caspase-7, Bax, Bcl-2, and β-Tubulin (control) in treated cells. p-Rb(S807/811) / Rb / CDK4 / CDK6 / Cyclin D1 / CDK2 / Cyclin E1; PubMed: 30959969 Molecules Western blot analysis of Rb/p-Rb, CDK4, CDK6, Cyclin D1, CDK2, Cyclin E1, and β-Tubulin (control) in the SK-Hep-1 and SNU-423 cells treated with ponatinib. RAD51; PubMed: 30959969 Molecules The effect of ponatinib on the concentration of Rad51 in SK-Hep-1 and SNU-423 cells. p-MEK / MEK / p-ERK / ERK; PubMed: 30959969 Molecules Effect of ponatinib on the phosphorylation of Mek and Erk. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was used as a control. c-Jun / p-JNK(T183/Y185) / JNK; PubMed: 30959969 Molecules Effect of ponatinib on the phosphorylation of JNK and c-Jun. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was used as a control. p-p38 MAPK / p38 MAPK; PubMed: 30959969 Molecules Effect of ponatinib on the phosphorylation of p38. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was used as a control. p-JAK2 / JAK2 / p-STAT3 / STAT3; PubMed: 30959969 Molecules Effect of ponatinib on the phosphorylation of Jak2, and Stat3. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was used as a control. PI3K p85 / p-PI3K / p-PDK1 / PDK1 / p-mTOR / mTOR; PubMed: 30959969 Molecules Effects of ponatinib on the protein concentration and phosphorylation of PI 3-kinase, PDK1, Akt, and mTOR in HCC cells. p-PDGFRα / PDGFRα; PubMed: 24472312 Mol Cancer Ponatinib inhibited phosphorylation of PDGFRα in a concentration-dependent manner. EOL-1 and BaF3-WT or -T674I FIP1L1-PDGFRα cells were exposed to escalating concentrations of ponatinib for 24 h. PARP / Caspase-3 / Active Caspase-3; PubMed: 24472312 Mol Cancer The concentration- (for 24 h) and time-dependent (1 nM for EOL-1, 300 nM for BaF3-WT and -T674I FIP1L1-PDGFRα cells) cleavage of PARP and caspase-3 triggered by ponatinib was analyzed by immunoblotting. AIF / Cyto C ; PubMed: 24472312 Mol Cancer Ponatinib elicited release of AIF and cytochrome c into the cytosol. Cells were treated with 1 nM ponatinib for the indicated durations and the cytosolic fraction was extracted with digitonin buffer. Levels of AIF and Cytochrome c (Cyto c) were detected by immunoblotting. Mcl-1 / Survivin / Bcl-Xl / XIAP / Bax / Bcl-2 / Bim; PubMed: 24472312 Mol Cancer Immunoblotting of apoptosis-related proteins in CEL cells after treatment for 24 h. β-catenin ; PubMed: 24472312 Mol Cancer Ponatinib concentration-dependently lowered β-catenin. EOL-1 cells were incubated with ponatinib for 24 h, and cytoplasmic and nuclear extracts were determined by immunoblotting. β-catenin; PubMed: 24472312 Mol Cancer Analysis of β-catenin localization. EOL-1 cells were pretreated with 1 nM ponatinib for 24 h, immunofluorescence analysis was performed with anti-β-catenin. Nuclei were stained with 4,6-diamidino-2-phenylindole (DAPI). ABL; PubMed: 19878872 Cancer Cell In vitro [γ-32P]-ATP autophosphorylation of full-length (A) ABL or (B) ABLT315I kinase treated with AP24534, imatinib, nilotinib, or dasatinib. After incubation of tyrosine-dephosphorylated enzyme with the indicated inhibitor in the presence of [γ-32P]-ATP and separation by SDS-PAGE, signal intensity was measured by autoradiography.	22781593 30959969 24472312 19878872
Growth inhibition assay	Cell viability; PubMed: 30174789 Oncotarget (A) Effect of Ponatinib on basal viability of HUVECs. The cells were treated with Ponatinib at indicated concentrations or 0.1% DMSO (vehicle control) for 24 h.	30174789

In vivo

In a mouse xenograft model of Ba/F3 cells expressing native Bcr-Abl, AP24534 (2.5 mg/kg and 5 mg/kg) prolongs mice median survival. In the xenograft model of Ba/F3 Bcr-Abl^T315I, AP24534 (10 mg/kg–50 mg/kg) significantly suppresses tumor growth. AP24534 (30 mg/kg) decreases the phosphorylated Bcr-Abl and phosphorylated CrkL levels in the tumors. ^[2]

Protocol

Kinase Assay:^[1]	- Collapse Peptide substrate phosphorylation assays with GST-Abl kinase domains: The effect of AP24534 (0-320 nM) on GST-Abl kinase activity is assessed by using a synthetic peptide substrate (Abltide: EAIYAAPFAKKK). Assays are carried out at 30 °C for 15 min in 25 μL reaction mixture: 8 mM MOPS (pH 7), 0.2 mM EDTA, 50 μM Abltide, 30 mM MgCl₂, 10 mM β-glycerol phosphate, 1 mM EGTA, 0.002% Brij-35, 0.4 mM DTT, 0.2 mg/mL BSA, 0.4 mM sodium orthovanadate, 10 nM WT or mutant GST-Abl kinase, and 100 µM ATP/γ-³²[P]ATP (5000 cpm/pmol). Reactions are terminated by transferring a portion of the reaction mixture onto a p81 phosphocellulose filter and immersing in 0.75% phosphoric acid. Filters are washed 3 times in 0.75% phosphoric acid, rinsed in acetone, and air dried; phosphate incorporation is determined by scintillation counting. All results are corrected for background binding to the filters, as determined by omitting peptide substrate from the kinase reaction. Time course experiments to establish the linear range of enzymatic activity precedes kinase assays.
Cell Research:^[2]	- Collapse Cell lines: Ba/F3 cells expressing Bcr-Abl or a range of single mutations in the kinase domain of Bcr-Abl Concentrations: 0-625 nM Incubation Time: 72 hours Method: Ba/F3 cell lines are distributed in 96-well plates (4 × 10³ cells/well) and incubated with AP24534 for 72 hours. Proliferation is measured using a methanethiosulfonate (MTS)-based viability assay (CellTiter96 Aqueous One Solution). All values are normalized to the control wells with no drug. IC50 values are reported as the mean of three independent experiments performed in quadruplicat (Only for Reference)
Animal Research:^[1]	- Collapse Animal Models: Mouse xenograft models of Ba/F3 cells expressing Bcr-Abl or Bcr-Abl^T315I Dosages: 2.5 mg/kg and 5 mg/kg for Ba/F3 Bcr-Abl; 2.5 mg/kg–50 mg/kg for Ba/F3 Bcr-Abl^T315I Administration: Oral gavage once daily (Only for Reference)

References

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Solubility (25°C)

In vitro	DMSO	30 mg/mL (56.33 mM)
	Water	Insoluble
	Ethanol	Insoluble
In vivo	Add solvents to the product individually and in order(Data is from Selleck tests instead of citations): 1% CMC Na For best results, use promptly after mixing.	30mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information Download Ponatinib (AP24534) SDF

Molecular Weight	532.56
Formula	C₂₉H₂₇F₃N₆O
CAS No.	943319-70-8
Storage	3 years-20°C powder 2 years-80°C in solvent
Synonyms	N/A
Smiles	CC1=C(C=C(C=C1)C(=O)NC2=CC(=C(C=C2)CN3CCN(CC3)C)C(F)(F)F)C#CC4=CN=C5N4N=CC=C5

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

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mg/kg

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% DMSO+ % + % Tween 80+ % ddH₂O

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Working concentration： mg/ml；

Method for preparing DMSO master liquid: ： mg drug pre-dissolved in μL DMSO (Master liquid concentration mg/mL， Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation：Take μL DMSO master liquid, next addμL PEG300， mix and clarify, next addμL Tween 80，mix and clarify, next add μL ddH₂O，mix and clarify.

Note：1.Please make sure the liquid is clear before adding the next solvent.
2.Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.

Bio Calculators

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (mg) = Concentration (mM) × Volume (mL) × Molecular Weight (g/mol)

*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and SDS / COA (available on product pages).

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Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

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This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

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The Serial Dilution Calculator Equation

Serial Dilutions
Concertration (start):
Dilution-folds:

Computed Result

C1=C0/X	C1:	LOG(C1):
C2=C1/X	C2:	LOG(C2):
C3=C2/X	C3:	LOG(C3):
C4=C3/X	C4:	LOG(C4):
C5=C4/X	C5:	LOG(C5):
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Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Clinical Trial Information (data from https://clinicaltrials.gov, updated on 2021-09-06)

NCT Number	Recruitment	interventions	Conditions	Sponsor/Collaborators	Start Date	Phases
NCT04554459	Recruiting	Drug: Ponatinib 15 MG Oral Tablet	Acute Lymphoblastic Leukemia Adult\|Ph+ ALL\|Newly Diagnosed	Institute of Hematology and Blood Transfusion Czech Republic\|CZECRIN - Czech Clinical Research Infrastructure Network	February 16 2021	Phase 2
NCT04160546	Recruiting	Drug: Ponatinib 15 MG\|Drug: Acetylsalicylic acid 100 MG	Chronic Myeloid Leukemia Chronic Phase	Fundacion CRIS de Investigación para Vencer el Cáncer\|Incyte Biosciences UK\|Apices Soluciones S.L.	January 17 2020	Phase 2
NCT03934372	Recruiting	Drug: Ponatinib	Acute Myeloid Leukemia\|Accelerated Phase Chronic Myeloid Leukemia\|Blast Phase Chronic Myeloid Leukemia\|Chronic Phase Chronic Myeloid Leukemia\|Acute Lymphoblastic Leukemia\|Acute Lymphocytic Leukemia\|Leukemia\|Lymphoma\|Solid Tumors	Incyte Biosciences International Sàrl\|Incyte Corporation	January 29 2020	Phase 1\|Phase 2
NCT03690115	Recruiting	Drug: Ponatinib 30 MG	Leukemia Myeloid Acute	Versailles Hospital	December 2 2019	Phase 2

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

Frequently Asked Questions

Question 1:
Is the pre-solubilized inhibitor suitable for in vivo whole animal studies? Whether the solvent contains any non-DMSO stabilizer?
Answer:
Our 10mM Ponatinib in DMSO solution is in pure DMSO and there is not other stabilizer added. You can use it for animal study. The vehicle we suggest for Ponatinib is: 30% PEG400/0.5% Tween80/5% Propylene glycol. It is a milky suspension used for oral gavage.

Bcr-Abl Signaling Pathway Map

Bcr-Abl Inhibitors with Unique Features

Selective Bcr-Abl Inhibitor

Nilotinib (AMN-107) : Bcr-Abl-selective, IC50<30 nM.
Most Potent Bcr-Abl Inhibitor

GZD824 : Bcr-Abl(WT), IC50=0.34 nM; Bcr-Abl(T315I), IC50=0.68 nM.
FDA-approved Bcr-Abl Inhibitor

Dasatinib : Approved by FDA for CML and Ph+ ALL.
Newest Bcr-Abl Inhibitor

GZD824 : Orally bioavailable Bcr-Abl inhibitor for Bcr-Abl(WT) and Bcr-Abl(T315I) with IC50 of 0.34 nM and 0.68 nM, respectively.

Related Bcr-Abl Products

PX-478 2HCl ^New

PX-478 2HCl is an orally active, and selective hypoxia-inducible factor-1α (HIF-1α) inhibitor. PX-478 2HCl induces apoptosis and has anti-tumor activity. Phase 1.
Defactinib (VS-6063) ^New

Defactinib (VS-6063, PF-04554878) is a selective, and orally active FAK inhibitor. Phase 2.
SU6656 ^New

SU 6656 is a selective Src family kinase inhibitor with IC50 of 280 nM, 20 nM, 130 nM, and 170 nM for Src, Yes, Lyn, and Fyn, respectively.
Imatinib (STI571) Mesylate

Imatinib (STI571, CGP057148B, Gleevec) Mesylate is an orally bioavailability mesylate salt of Imatinib, which is a multi-target inhibitor of v-Abl, c-Kit and PDGFR with IC50 of 0.6 μM, 0.1 μM and 0.1 μM in cell-free or cell-based assays, respectively. Imatinib Mesylate (STI571) induces autophagy.
Nilotinib (AMN-107)

Nilotinib (AMN-107) is a selective Bcr-Abl inhibitor with IC50 less than 30 nM in Murine myeloid progenitor cells. Nilotinib induces autophagy through AMPK activition.

Features:A selective inhibitor of native and mutant Bcr-Abl.
Bafetinib (INNO-406)

Bafetinib (INNO-406, NS-187) is a potent and selective dual Bcr-Abl/Lyn inhibitor with IC50 of 5.8 nM/19 nM in cell-free assays, does not inhibit the phosphorylation of the T315I mutant and is less potent to PDGFR and c-Kit. Phase 2.

Features:Dual Bcr-Abl/Lyn inhibitor.
Degrasyn (WP1130)

Degrasyn (WP1130) is a selective deubiquitinase (DUB: USP5, UCH-L1, USP9x, USP14, and UCH37) inhibitor and also suppresses Bcr/Abl, also a JAK2 transducer (without affecting 20S proteasome) and activator of transcription (STAT). Degrasyn (WP1130) induces apoptosis and blocks autophagy.

Features:WP1130 has an advantage over imatinib mesylate in that its activity is not inhibited by a variety of Abl kinase mutations, including T315I.
Rebastinib (DCC-2036)

Rebastinib (DCC-2036) is a conformational control Bcr-Abl inhibitor for Abl1(WT) and Abl1(T315I) with IC50 of 0.8 nM and 4 nM, also inhibits SRC, LYN, FGR, HCK, KDR, FLT3, and Tie-2, and low activity to seen towards c-Kit. Phase 1.

Features:A conformational control inhibitor of Abl1 and T315I Abl1.
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Olverembatinib dimesylate (HQP1351, GZD824) is a novel orally bioavailable Bcr-Abl inhibitor for Bcr-Abl(WT) and Bcr-Abl(T315I) with IC50 of 0.34 nM and 0.68 nM, respectively.

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Ponatinib (AP24534)