Ponatinib (AP24534)

Catalog No.S1490

Ponatinib (AP24534) Chemical Structure

Molecular Weight(MW): 532.56

Ponatinib (AP24534) is a novel, potent multi-target inhibitor of Abl, PDGFRα, VEGFR2, FGFR1 and Src with IC50 of 0.37 nM, 1.1 nM, 1.5 nM, 2.2 nM and 5.4 nM in cell-free assays, respectively.

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Cited by 36 Publications

Purity & Quality Control

Choose Selective Bcr-Abl Inhibitors

Biological Activity

Description Ponatinib (AP24534) is a novel, potent multi-target inhibitor of Abl, PDGFRα, VEGFR2, FGFR1 and Src with IC50 of 0.37 nM, 1.1 nM, 1.5 nM, 2.2 nM and 5.4 nM in cell-free assays, respectively.
Targets
Abl [1]
(Cell-free assay)
PDGFRα [1]
(Cell-free assay)
VEGFR2 [1]
(Cell-free assay)
FGFR1 [1]
(Cell-free assay)
c-Src [1]
(Cell-free assay)
0.37 nM 1.1 nM 1.5 nM 2.2 nM 5.4 nM
In vitro

AP24534 potently inhibits native Abl, AblT315I, and other clinically important Abl kinase domain mutants with IC50 of 0.30 nM–2 nM. AP24534 doesn't inhibit Aurora kinase family members, nor does it inhibit insulin receptor or CDK2/cyclin E. AP24534 inhibits proliferation of Ba/F3 cells expressing Bcr-Abl with IC50 of 0.5 nM, as well as Ba/F3 cells expressing a range of Bcr-Abl mutants with IC50 of 0.5 nM–36 nM. The inhibition of proliferation by AP24534 is correlated with induction of apoptosis. [1-2] In leukemic cell lines containing activated forms of FLT3, KIT, FGFR1, and PDGFRα receptors, AP24534 potently inhibits receptor phosphorylation and cellular proliferation with IC50 of 0.3 nM to 20 nM. In MV4-11 (FLT3-ITD(+/+)) but not RS4;11 (FLT3-ITD(–/–)) AML cells, AP24534 inhibits FLT3 signaling and induced apoptosis at less than 10 nM. AP24534 inhibits viability of primary leukemic blasts from a FLT3-ITD positive AML patient with IC50 of 4 nM but not those from patients with AML expressing native FLT3. [3] In Ba/F3 cells engineered to express activated FGFR1-4, AP24534 potently inhibits FGFR-mediated signaling and viability with IC50 lower than 40 nM. In cell lines representing multiple tumor types (endometrial, bladder, gastric, breast, lung, and colon), and containing FGFRs dysregulated by a variety of mechanisms, AP24534 inhibits FGFR-mediated signaling with IC50 less than 40 nM and inhibits cell growth with IC50 of 7 nM–181 nM. [4]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
EoL-1-cell MVfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYfJR|UxRTBwMECwNFAxODN3OTFOwG0> MVnTRW5ITVJ?
MV-4-11 M13Tcmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NYHKPWZ1UUN3ME2wMlAxODB7NU[g{txO M1v1cnNCVkeHUh?=
MEG-01 M4LTe2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mn[zTWM2OD1yLkCwNFEzQCEQvF2= NX7DXodxW0GQR1XS
LAMA-84 NV3yRmlnT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3e3eWlEPTB;MD6wNFAyPjVizszN M2e3OXNCVkeHUh?=
CGTH-W-1 NXrMZoVmT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVTYPGRMUUN3ME2wMlAxOTZ7IN88US=> NVnUeGd5W0GQR1XS
KASUMI-1 MVnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NInQenRKSzVyPUCuNFAzOTdizszN MV7TRW5ITVJ?
BE-13 NIrBeppIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1\1Z2lEPTB;MD6wNFI2QSEQvF2= NWDqTI85W0GQR1XS
CTV-1 M{XpPWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MmPyTWM2OD1yLkCwOVIyKM7:TR?= NVLuRolYW0GQR1XS
NKM-1 M3rReGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M2XIOGlEPTB;MD6wNFY2PSEQvF2= MXTTRW5ITVJ?
MONO-MAC-6 M{fke2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXXJR|UxRTBwMEC4N|ch|ryP M2nQVXNCVkeHUh?=
A204 NFWwXWdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MmLYTWM2OD1yLkCxNlE5KM7:TR?= M{jSUnNCVkeHUh?=
GDM-1 M{PhZWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NILKUpZKSzVyPUCuNFE5OzNizszN M2XlbHNCVkeHUh?=
AN3-CA MWHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4XENGlEPTB;MD6wNVg4OyEQvF2= M3LFSXNCVkeHUh?=
BL-70 M33FU2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MWTJR|UxRTBwMEKxPVkh|ryP M2rmVXNCVkeHUh?=
Daudi M2O1VGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M4rlRWlEPTB;MD6wNlU{PyEQvF2= NI\nZ|FUSU6JRWK=
KU812 NGrybnFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2GyemlEPTB;MD6wNlg1QCEQvF2= MmSyV2FPT0WU
KG-1 MYLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWPsdZBlUUN3ME2wMlA{Ojl2IN88US=> NHXnbZlUSU6JRWK=
DOHH-2 Mlu1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NYjqTmxGUUN3ME2wMlA1QDB|IN88US=> MnHwV2FPT0WU
HuO9 NX3EU4l2T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1\0eGlEPTB;MD6wOVQ1QCEQvF2= M3H6UnNCVkeHUh?=
EB2 MV7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Ml;KTWM2OD1yLkC1OVc3KM7:TR?= M3vNcXNCVkeHUh?=
G-402 M2fjWmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MVnJR|UxRTBwME[zOFEh|ryP NWHSfplSW0GQR1XS
SU-DHL-1 M3nWSmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVTnOmN3UUN3ME2wMlA3Ozd7IN88US=> NUjIe4k4W0GQR1XS
NCI-H719 NYfmeVNTT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVPBfJlxUUN3ME2wMlA4QDh6IN88US=> MWfTRW5ITVJ?
MLMA NWezSZhkT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MnjGTWM2OD1yLkC5NFYh|ryP MXzTRW5ITVJ?
KM12 NV2xPYQyT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXj0NlcyUUN3ME2wMlA6PzN5IN88US=> NFHLWnRUSU6JRWK=
SUP-T1 MmDJS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MnjiTWM2OD1yLkC5PVgzKM7:TR?= M{XXZXNCVkeHUh?=
MZ1-PC NHzuRmhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2D1Z2lEPTB;MD6xNVM{PiEQvF2= M2HURXNCVkeHUh?=
MFE-296 NY\p[ZA{T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NEjOUIdKSzVyPUCuNVE5OzlizszN NUXsbpY3W0GQR1XS
ALL-PO MkTLS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3zj[GlEPTB;MD6xNlQ1OyEQvF2= Mkj6V2FPT0WU
GB-1 M2SxXWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NEfFT2RKSzVyPUCuNVI2OTJizszN NE\FVYlUSU6JRWK=
SIG-M5 M3nUbmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1zXWmlEPTB;MD6xN|M4KM7:TR?= M1P0O3NCVkeHUh?=
P30-OHK NEP0RYJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1XDOmlEPTB;MD6xOVY4OiEQvF2= NEPCPJlUSU6JRWK=
NCI-H716 M2K3Rmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MknaTWM2OD1yLkG2NlY3KM7:TR?= NFvvVmFUSU6JRWK=
MPP-89 M3LBZmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MYXJR|UxRTBwMU[5N|Mh|ryP M{DxRnNCVkeHUh?=
SF126 Mn;YS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M362bGlEPTB;MD6xO|A3OyEQvF2= NEnmSZlUSU6JRWK=
CTB-1 NW\1elk6T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1:1fmlEPTB;MD6xO|MyPiEQvF2= M1GwS3NCVkeHUh?=
CA46 MX7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NG[wNXNKSzVyPUCuNVc1QDlizszN MkPrV2FPT0WU
NCI-H520 MlHYS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MUDJR|UxRTBwMUe3N|Ih|ryP MXXTRW5ITVJ?
CAL-85-1 NG\nZ4dIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NHvWPG1KSzVyPUCuNVc6OjdizszN M3WySXNCVkeHUh?=
NCI-H2342 M33RWmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MofsTWM2OD1yLkG4OVc4KM7:TR?= MWHTRW5ITVJ?
697 MXTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NGn0VJZKSzVyPUCuNVg5PzFizszN MWHTRW5ITVJ?
NY NYnWcGQ2T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MUXJR|UxRTBwMUmyPFch|ryP NIXtNWVUSU6JRWK=
T98G MVrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYLJR|UxRTBwMUmzNVgh|ryP M2DsfnNCVkeHUh?=
NCI-H1703 MnnMS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MWTJR|UxRTBwMUm2NVUh|ryP M{DUe3NCVkeHUh?=
NCI-H2087 NUDEUnhRT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NWXGXHc4UUN3ME2wMlIxPDN7IN88US=> MVXTRW5ITVJ?
PF-382 MV\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYHJR|UxRTBwMkC4O|Mh|ryP MVrTRW5ITVJ?
HCT-116 NEXHUWNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MW\JR|UxRTBwMkGwOlkh|ryP MmexV2FPT0WU
CAKI-1 MUnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVTJR|UxRTBwMkG2OlYh|ryP NGnPSZdUSU6JRWK=
CCF-STTG1 MUTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NX7Z[ZB1UUN3ME2wMlIzPDd3IN88US=> M3fZUHNCVkeHUh?=
D-392MG MVnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4j4VmlEPTB;MD6yN|E4PyEQvF2= MoTEV2FPT0WU
RT-112 Ml3kS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Ml;6TWM2OD1yLkKzO|k2KM7:TR?= NXq1eVk2W0GQR1XS
MES-SA NGPoVHBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXWzVFB{UUN3ME2wMlI1OTh5IN88US=> M1W2dHNCVkeHUh?=
LC-2-ad NILTR3JIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVPMVJFGUUN3ME2wMlI1OjZ3IN88US=> NWPBU3A6W0GQR1XS
NOS-1 NGHz[HhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MmjyTWM2OD1yLkK0N|Y4KM7:TR?= NWrjfncxW0GQR1XS
NALM-6 MlTuS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MWTJR|UxRTBwMkW1OFMh|ryP NGrFT|ZUSU6JRWK=
TE-15 MnXCS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NEDuZ4dKSzVyPUCuNlYxQTFizszN M4DafXNCVkeHUh?=
786-0 MlGzS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NX\Jb5loUUN3ME2wMlI3OjJ6IN88US=> NVTES|JrW0GQR1XS
IST-MES1 M3j3NGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NYnOeYxmUUN3ME2wMlI3QDJ7IN88US=> NYDlV21YW0GQR1XS
KYSE-180 MVjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWP3SVFMUUN3ME2wMlI3QDlzIN88US=> MlTUV2FPT0WU
YT MX7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVjQT|JxUUN3ME2wMlI3QTd2IN88US=> MWDTRW5ITVJ?
A427 M4rHSmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M4DyV2lEPTB;MD6yO|I3PCEQvF2= M1HYWXNCVkeHUh?=
KYSE-450 M37aT2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVG4XlNnUUN3ME2wMlI4OzJ7IN88US=> M{PibnNCVkeHUh?=
HuH-7 MWrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mo\xTWM2OD1yLkK3O|Y{KM7:TR?= NV7ENYVpW0GQR1XS
CAS-1 MYrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MoLaTWM2OD1yLkK4N|A{KM7:TR?= NHG3bIFUSU6JRWK=
KURAMOCHI M4rNT2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MUfJR|UxRTBwMki0NVkh|ryP NYXkSXBzW0GQR1XS
ACHN M3zkbmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NI\CNYZKSzVyPUCuNlk{OjNizszN MWfTRW5ITVJ?
HGC-27 M{HTNWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NGnt[Y9KSzVyPUCuNlk{PTNizszN NFrJeIdUSU6JRWK=
A172 NUHoVW9nT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MV7JR|UxRTBwMkm1O|ch|ryP M1rUNXNCVkeHUh?=
SNU-449 M{L4WGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NE\KbnlKSzVyPUCuNlk6PjRizszN NVfsfZk1W0GQR1XS
SW982 Mn3wS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MlLJTWM2OD1yLkK5PVc5KM7:TR?= MkO5V2FPT0WU
EFO-21 MVrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MnHFTWM2OD1yLkOwOFEyKM7:TR?= MkLBV2FPT0WU
NCI-H2030 NXfjcY12T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MVXJR|UxRTBwM{GxOlQh|ryP Mk[3V2FPT0WU
HT-144 M1[wWmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NWT1fIxuUUN3ME2wMlMyOjhizszN NILRSXNUSU6JRWK=
MFE-280 MX;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2XQVmlEPTB;MD6zNVQ2OyEQvF2= M4XYfnNCVkeHUh?=
SK-LU-1 NHTTTWRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MmDDTWM2OD1yLkOxOFY3KM7:TR?= M1TZ[nNCVkeHUh?=
NCI-H2052 NGrrZZNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXTJR|UxRTBwM{G0PFQh|ryP NV74bGZCW0GQR1XS
SK-HEP-1 NVn1[Jh[T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M{\FbWlEPTB;MD6zN|M3QCEQvF2= NH7ERlRUSU6JRWK=
QIMR-WIL M{DqU2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M{W3S2lEPTB;MD6zN|QzPCEQvF2= MYPTRW5ITVJ?
A2780 NETr[nVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYDJR|UxRTBwM{SzNFQh|ryP NU\ZVG5CW0GQR1XS
ACN NEiycYlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXrJR|UxRTBwM{S4O|Mh|ryP MkXCV2FPT0WU
BxPC-3 MUDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVL2NVVTUUN3ME2wMlM1QTh7IN88US=> NXrye5ZlW0GQR1XS
BCPAP MYfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4fHOGlEPTB;MD6zOlAxOSEQvF2= NEntXHhUSU6JRWK=
HOP-62 NE\GfFhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1[xdWlEPTB;MD6zOlExPiEQvF2= M375[HNCVkeHUh?=
SW1710 NH;HNGZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1PqUWlEPTB;MD6zOlQ2PSEQvF2= M17uUHNCVkeHUh?=
OPM-2 Ml\nS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1zqcWlEPTB;MD6zOlQ4OyEQvF2= MV\TRW5ITVJ?
DBTRG-05MG MUDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NUjnV25TUUN3ME2wMlM5ODV2IN88US=> M2fNSHNCVkeHUh?=
SK-MEL-24 M1HMVmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NY\2[5hrUUN3ME2wMlM6PzlzIN88US=> MUjTRW5ITVJ?
8305C NXvVRWRWT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MVzJR|UxRTBwM{m3PVMh|ryP MmKxV2FPT0WU
OVCAR-4 Mo\hS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NF\POFJKSzVyPUCuOFE2QDZizszN M1XiSnNCVkeHUh?=
HLE NV3zbYVIT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4LGXWlEPTB;MD60NVg3KM7:TR?= NUPO[nQ3W0GQR1XS
NCI-H747 MWLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3PHSGlEPTB;MD60NlE{PyEQvF2= MkLSV2FPT0WU
SW780 NYXTWWt7T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NYnpU2luUUN3ME2wMlQzOzR4IN88US=> MWjTRW5ITVJ?
KYSE-150 MonTS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MknOTWM2OD1yLkSyOFE2KM7:TR?= M2jq[HNCVkeHUh?=
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SW1088 M1fGcGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MlrFTWM2OD1yLkS0N|kh|ryP Mn3oV2FPT0WU
SF268 MnPGS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NIHnd5BKSzVyPUCuOFQ5PiEQvF2= NYfCUI9nW0GQR1XS
RT4 M1;XeGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXXxbWRuUUN3ME2wMlQ2OTFzIN88US=> NGHReGFUSU6JRWK=
NCI-H522 MlW4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NIrST41KSzVyPUCuOFUzPyEQvF2= NEToZ2dUSU6JRWK=
LCLC-97TM1 MkLSS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NX22b|FQUUN3ME2wMlQ3OTl3IN88US=> NHXs[oNUSU6JRWK=
CAL-39 MUfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MUHJR|UxRTBwNE[1NVkh|ryP MVvTRW5ITVJ?
CAL-120 M{P1T2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NV\sSVk1UUN3ME2wMlQ4PjR|IN88US=> MlziV2FPT0WU
ESS-1 M1G3XGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MlvuTWM2OD1yLkS3PFIyKM7:TR?= MUHTRW5ITVJ?
KARPAS-45 NIPSVIlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mm\4TWM2OD1yLkS4PVM{KM7:TR?= Mor5V2FPT0WU
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VMRC-RCZ NUTwZ|JvT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MkntTWM2OD1yLkS5PVA1KM7:TR?= MoDWV2FPT0WU
HOS MYXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NFvwVIZKSzVyPUCuOVA{PjVizszN NU\YbopvW0GQR1XS
MOLT-16 NHLGUWlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXT5fos4UUN3ME2wMlUyQDB6IN88US=> MoThV2FPT0WU
RPMI-6666 NUfITY9XT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MY\JR|UxRTBwNUKxN|Uh|ryP M17kW3NCVkeHUh?=
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OVCAR-5 MWLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MUDJR|UxRTFwOUC2O|Uh|ryP MUDTRW5ITVJ?
SNG-M MULHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NI\RXnJKSzVyPUGuPVU5PThizszN MX3TRW5ITVJ?
A673 MYLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MlS1TWM2OD1zLkm3OVE3KM7:TR?= NYPwell{W0GQR1XS
L-428 MYLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NYq5XWhJUUN3ME2xMlk6OTd7IN88US=> NHjnNoRUSU6JRWK=
KM-H2 M4fRemdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHPCOYpKSzVyPUKuNFExQSEQvF2= MYPTRW5ITVJ?
MC-IXC MlzqS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NF;idVFKSzVyPUKuNFIzPzNizszN MlXUV2FPT0WU
COLO-678 MnnGS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mn[xTWM2OD1{LkC0NlA4KM7:TR?= Mln0V2FPT0WU
MEL-JUSO NH\sSldIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXfJR|UxRTJwMEe4OVEh|ryP MkH2V2FPT0WU
HCC38 NETBOnZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MnTmTWM2OD1{LkC4OFM{KM7:TR?= NH\CXpZUSU6JRWK=
UACC-62 NVLlcodqT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Ml3nTWM2OD1{LkC4PFU2KM7:TR?= MljiV2FPT0WU
OAW-42 NXHITZZUT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NU[2XXd1UUN3ME2yMlA6PzFzIN88US=> Mm\aV2FPT0WU
LB996-RCC NIDw[G5Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3r2d2lEPTB;Mj6xN|gyPyEQvF2= NVq3fVVwW0GQR1XS
COR-L105 MUjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MkjDTWM2OD1{LkG2N|c{KM7:TR?= MWXTRW5ITVJ?
RCC10RGB NWPZSoQ{T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MmnxTWM2OD1{LkG4OVc3KM7:TR?= MnGzV2FPT0WU
A431 NVTYOopWT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NUPETpBGUUN3ME2yMlE6ODF7IN88US=> NGHFUoFUSU6JRWK=
NCI-H292 NIryNYhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NYjY[ZJ4UUN3ME2yMlI{QTVzIN88US=> NX;nXJJRW0GQR1XS
SK-UT-1 NInIT41Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mo\UTWM2OD1{LkOzNVg1KM7:TR?= M13XNnNCVkeHUh?=
PANC-08-13 NEHnRXlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NWP5UW0yUUN3ME2yMlM{OjN3IN88US=> MVLTRW5ITVJ?
ST486 M2\ke2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MmW5TWM2OD1{LkSxNFQ5KM7:TR?= MkHIV2FPT0WU
SJSA-1 Mn\iS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NV7XVXJ[UUN3ME2yMlQyOjdzIN88US=> M3vCW3NCVkeHUh?=
RKO NUHZXm5[T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MoP4TWM2OD1{LkSyNFI6KM7:TR?= NUDTPXBiW0GQR1XS
NCI-H1793 Mn;0S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NHS3bm5KSzVyPUKuOFQxOjlizszN MoD4V2FPT0WU
639-V NYX5T2Q2T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M2PzeWlEPTB;Mj60OVM5OiEQvF2= M{HWenNCVkeHUh?=
ES3 M2P0Umdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NWLsVoZLUUN3ME2yMlQ6PzN5IN88US=> NY\FTXQ6W0GQR1XS
NCI-H1792 NYWwZZdYT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3LMdmlEPTB;Mj61OlgzPiEQvF2= MUPTRW5ITVJ?
NCI-H1651 MWTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NXHh[4sxUUN3ME2yMlU5OjFizszN NGnBdpRUSU6JRWK=
SN12C NYrHfVhPT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MVHJR|UxRTJwNUi0PFch|ryP M2LrUXNCVkeHUh?=
COLO-679 NGTEeGZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NH\pW3FKSzVyPUKuOVg3ODVizszN MlPvV2FPT0WU
U-118-MG NX6xZ2dPT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MoLBTWM2OD1{LkW4PFczKM7:TR?= NX2zO|I1W0GQR1XS
Ramos-2G6-4C10 NIS3WGxIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NEXrRXpKSzVyPUKuOlQ5ODdizszN NELkblZUSU6JRWK=
HCT-15 MkjOS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MYjJR|UxRTJwNki3PVIh|ryP NVHITG1OW0GQR1XS
MDA-MB-157 NWfTZZpqT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MkP4TWM2OD1{LkewO|Q4KM7:TR?= NV\Jdpg1W0GQR1XS
K5 NGWwd5RIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NFnlRpVKSzVyPUKuO|E5PDVizszN MXjTRW5ITVJ?
MKN45 NXLtXpozT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MXvJR|UxRTJwN{OzOlYh|ryP NX\Ne|B4W0GQR1XS
DMS-273 NH\qPIRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mkn3TWM2OD1{Lke1OlMzKM7:TR?= M3XOO3NCVkeHUh?=
C8166 NES3RpFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MoH1TWM2OD1{Lke4OVE{KM7:TR?= M1TXdHNCVkeHUh?=
Calu-3 MlziS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXn6UG1VUUN3ME2yMlc5QTZ7IN88US=> MlzxV2FPT0WU
SNB75 NFK5WY5Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NIT0coxKSzVyPUKuPFg2OzVizszN MUHTRW5ITVJ?
BFTC-905 M372Tmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MVTJR|UxRTJwOUCxPVEh|ryP M4LIWXNCVkeHUh?=
C-4-II NXS4[nZvT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXnpUoZuUUN3ME2yMlkyOzV|IN88US=> NHTF[nVUSU6JRWK=
MS-1 MYjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXnJR|UxRTJwOU[3Olkh|ryP Ml7HV2FPT0WU
A375 NETMVGtIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NFnzUIVKSzVyPUOuNFgyQDRizszN M3;YfXNCVkeHUh?=
KYSE-70 NVO4[HhRT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1jKcWlEPTB;Mz6wPFg5OiEQvF2= NEjo[nZUSU6JRWK=
KYSE-510 MYHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3rifmlEPTB;Mz6xNFk6PSEQvF2= NYnafZpFW0GQR1XS
LN-405 M4jLXGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHnKeGZKSzVyPUOuNVM2OThizszN MX3TRW5ITVJ?
BOKU NH\UcY5Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2Sz[GlEPTB;Mz6xOFI2PiEQvF2= NH;qTIRUSU6JRWK=
TE-10 M2LWS2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MWXJR|UxRTNwMUezOlIh|ryP M1vzTnNCVkeHUh?=
MKN1 NYnaOHo2T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHzPbHpKSzVyPUOuNVg1QDNizszN Mn;wV2FPT0WU
M059J M1zMW2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MnvxTWM2OD1|LkKwNVc{KM7:TR?= NH\k[FJUSU6JRWK=
GOTO M4TjRmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mn\KTWM2OD1|LkKyNFM6KM7:TR?= MYPTRW5ITVJ?
DK-MG MXLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWDXb3R6UUN3ME2zMlI2QTd4IN88US=> NGG5V4xUSU6JRWK=
NCI-H727 M{f0bmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXnJR|UxRTNwMk[1PEDPxE1? NX;KdINqW0GQR1XS
NB12 Mn\JS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXzQWYM{UUN3ME2zMlM{OjJ2IN88US=> NV\UNHFnW0GQR1XS
DOK MkDvS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2CyTWlEPTB;Mz6zOlE5PiEQvF2= MYrTRW5ITVJ?
KYSE-410 NXvp[Y9HT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NUnabotPUUN3ME2zMlM3Ojh5IN88US=> M1vUUnNCVkeHUh?=
AM-38 NYThNpR7T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3rJNWlEPTB;Mz6zPFA2OSEQvF2= NGTNZZdUSU6JRWK=
GR-ST NV2wPIxDT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MV3JR|UxRTNwNEW3N|Ih|ryP Mkn1V2FPT0WU
D-247MG MUPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NYHEcG84UUN3ME2zMlQ5OjZ|IN88US=> Ml:yV2FPT0WU
TE-8 NIDoW|BIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NHrlTo9KSzVyPUOuOFk{OzNizszN NHP4R|lUSU6JRWK=
LS-411N MUPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MlnZTWM2OD1|LkS5OlkzKM7:TR?= NGDPcnlUSU6JRWK=
SK-MES-1 NX7YUGs3T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MlHZTWM2OD1|LkW2OFM{KM7:TR?= NUnkcIZlW0GQR1XS
SF295 MV3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NIK5ZWdKSzVyPUOuOlE2PTJizszN NIPmTlFUSU6JRWK=
SK-MEL-30 Ml7ES5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NIXJfllKSzVyPUOuOlU6OjFizszN MlO5V2FPT0WU
LB2241-RCC M1fye2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MUHJR|UxRTNwNk[yOkDPxE1? M1f5ZXNCVkeHUh?=
PC-14 NVfIRWRNT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1fuemlEPTB;Mz62OlQ{PyEQvF2= NGLINlBUSU6JRWK=
EW-7 M{LkUWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M37wVWlEPTB;Mz62O|Y6OyEQvF2= NGDPNYhUSU6JRWK=
NMC-G1 M4j4PGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M2TMOmlEPTB;Mz63NVg{PyEQvF2= MX7TRW5ITVJ?
TE-441-T NUXFVWI2T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MlKwTWM2OD1|LkezNFI5KM7:TR?= NHjxdYtUSU6JRWK=
NCI-H1618 M1zrOWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MVjJR|UxRTNwOEG4O|Uh|ryP MVjTRW5ITVJ?
PANC-03-27 MYXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVi2fWJtUUN3ME2zMlgyQTF4IN88US=> NE\pXHhUSU6JRWK=
NCI-H2228 NYHU[2NpT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NF[3XItKSzVyPUOuPFMyOjZizszN NVnOSVV{W0GQR1XS
CAPAN-1 M3:zZmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MVTJR|UxRTNwOEewNFkh|ryP NU[yW5ZpW0GQR1XS
KLE MoX4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NUDI[I1OUUN3ME2zMlkxPjdizszN M3TufXNCVkeHUh?=
Becker MVfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MljtTWM2OD1|LkmyPFQ6KM7:TR?= NGDXUJBUSU6JRWK=
SK-PN-DW MXnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M37NSGlEPTB;ND6wO|k4PCEQvF2= M2rsWnNCVkeHUh?=
Hs-578-T NG\UdpZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVfndnR3UUN3ME20MlA5OzB4IN88US=> MmLDV2FPT0WU
RS4-11 MYDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWXtZ3Z1UUN3ME20MlEyOjV3IN88US=> NFLMd|VUSU6JRWK=
D-502MG NF[2bo9Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2TDZWlEPTB;ND6xOlk6PyEQvF2= M{jqRnNCVkeHUh?=
GI-1 NYPHUIpxT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NIiwNoNKSzVyPUSuNlgzQTlizszN M2fNVXNCVkeHUh?=
NCI-H209 MkXPS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NY\GTW5pUUN3ME20MlM{PzhizszN NEWzSnpUSU6JRWK=
SK-OV-3 MU\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NGnEc3JKSzVyPUSuOFgyPDlizszN M{TRdXNCVkeHUh?=
LOUCY NV\4NZZ1T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MYTJR|UxRTRwNEi0N|Ih|ryP Ml;vV2FPT0WU
NCI-H69 NXTKcYVuT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MkDKTWM2OD12LkWxO|k{KM7:TR?= NFXDPGpUSU6JRWK=
SCC-9 NHTPdlFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NFj4c4hKSzVyPUSuOVMyPzJizszN NH\hbGlUSU6JRWK=
NCI-H526 MkjLS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M{[zO2lEPTB;ND61PVI6OyEQvF2= MlHVV2FPT0WU
NCI-H2170 NEnkW|lIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXfJR|UxRTRwNk[3O|Eh|ryP MVXTRW5ITVJ?
SR NVP2cYtqT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M2HrOmlEPTB;ND63NFA1QCEQvF2= M1vtW3NCVkeHUh?=
CMK M1TBdGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1e0RmlEPTB;ND63NlExOSEQvF2= NGfLeYtUSU6JRWK=
COLO-829 Mn7kS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NEDwSVJKSzVyPUSuO|cxPDNizszN MoXNV2FPT0WU
HuO-3N1 NFXWVJRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M{XLWWlEPTB;ND64N|I5OSEQvF2= M2m1PHNCVkeHUh?=
5637 NWW3cY02T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NYTPXHp4UUN3ME20MlkxPDh5IN88US=> M4TqS3NCVkeHUh?=
A253 NV74TY1IT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4TIe2lEPTB;ND65O|cxQCEQvF2= NFniS|VUSU6JRWK=
NB10 M{XpSmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NIfH[25KSzVyPUWuNFc1QTdizszN M1[yTXNCVkeHUh?=
SF539 M33aPGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MUPJR|UxRTVwMEm5OVkh|ryP M2exNHNCVkeHUh?=
HAL-01 NHvpeWtIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXTl[IpyUUN3ME21MlE1PjR3IN88US=> MX\TRW5ITVJ?
MHH-ES-1 MY\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MUXJR|UxRTVwMU[5NlUh|ryP MYjTRW5ITVJ?
DEL MY\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NXfhVYlLUUN3ME21MlI4PTh{IN88US=> MnuxV2FPT0WU
NCI-H1299 MWTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHTGOVJKSzVyPUWuN|UxODdizszN NV;Lb4RSW0GQR1XS
ChaGo-K-1 NFv2NFJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4HacmlEPTB;NT6zOVExPyEQvF2= M171[XNCVkeHUh?=
HMV-II NULST2toT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MnnUTWM2OD13LkO5OFMh|ryP NWrWTHZ[W0GQR1XS
NB69 NFvKbYJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NWHUfWx6UUN3ME21MlM6PjNzIN88US=> M3;RfnNCVkeHUh?=
NCI-H1882 MnnKS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3r0bWlEPTB;NT60N|M3PCEQvF2= MkjRV2FPT0WU
SNU-475 NF;RfWdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NF7z[YdKSzVyPUWuOFcyPzFizszN NFrVZnZUSU6JRWK=
RXF393 MYjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NFXBVZhKSzVyPUWuOFg1QTRizszN M3LF[3NCVkeHUh?=
CAL-51 MYfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHnmR|BKSzVyPUWuOlI5PTNizszN Mn7kV2FPT0WU
SW1573 MVrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MoPnTWM2OD13Lk[zOVM1KM7:TR?= MWPTRW5ITVJ?
GCT MUfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3\5OmlEPTB;NT63OVkzQSEQvF2= MlfKV2FPT0WU
COR-L88 MUHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVuxSpVDUUN3ME21Mlc3QDR4IN88US=> MoTlV2FPT0WU
MDA-MB-134-VI Ml;RS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NGLTbI1KSzVyPUWuPFc2OzZizszN M{TLO3NCVkeHUh?=
NB5 NG[0d2dIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NI\vTmdKSzVyPUWuPVI1PCEQvF2= MYjTRW5ITVJ?
GMS-10 NHTV[WVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NUDtVXAyUUN3ME21Mlk3QDZzIN88US=> MkOzV2FPT0WU
CHP-212 NEDjfm1Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXnJR|UxRTZwNEiyOlch|ryP M3fIeHNCVkeHUh?=
TE-11 NIq1SFZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NFXzZpJKSzVyPU[uOVUyQDJizszN MXrTRW5ITVJ?
SW962 NVLscGJ6T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHXZZoNKSzVyPU[uOlU4PzRizszN MVfTRW5ITVJ?
MZ2-MEL M2XJbWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFTofGtKSzVyPU[uPFIxOTFizszN M2LZd3NCVkeHUh?=
C-33-A MnnmS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NUfvb3d{UUN3ME22MlkyPjh6IN88US=> NFzuVYhUSU6JRWK=
IGR-1 Mme4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NIHJemlKSzVyPU[uPVc5OjZizszN MY\TRW5ITVJ?
MOLT-4 M{L0Tmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MX3JR|UxRTdwMUW0NFYh|ryP NHqyNJJUSU6JRWK=
NCI-H1650 NX3kcVFFT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NUHqb2JWUUN3ME23MlI4ODR4IN88US=> MWnTRW5ITVJ?
AsPC-1 NHPHT5FIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NYHKTI5XUUN3ME23MlI5PjB3IN88US=> M3Xse3NCVkeHUh?=
SNU-C2B M1TheGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXnvem8xUUN3ME23MlI6PTdzIN88US=> MonxV2FPT0WU
MKN7 M1jvR2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MX;JR|UxRTdwM{eyN|Uh|ryP NIj6OoxUSU6JRWK=
SW620 NX;ROFV3T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MVzJR|UxRTdwM{iyNFUh|ryP MWPTRW5ITVJ?
SK-MEL-28 MmSzS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3TNUmlEPTB;Nz60NFc6QCEQvF2= NXPVPJNtW0GQR1XS
TE-6 NWTsVYdUT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Moq3TWM2OD15LkS0PVA5KM7:TR?= Mof0V2FPT0WU
D-263MG MoWzS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Ml7MTWM2OD15LkS1O|YzKM7:TR?= M13kd3NCVkeHUh?=
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BB65-RCC MmKxS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MlHaTWM2OD1{Mz6wN|Q1KM7:TR?= MmPMV2FPT0WU
SiHa M1XTPGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NWL4UYNyUUN3ME2yN{4{QTZ6IN88US=> MonCV2FPT0WU
NCI-H2081 M3zQdGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXrZeHpFUUN3ME2yOE4xPjRzIN88US=> NXnaV|JNW0GQR1XS
U251 M{LZVmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MUPJR|UxRTJ2LkC2Olgh|ryP Mn;WV2FPT0WU
LoVo M1vwbGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXHJR|UxRTJ2LkmyPFIh|ryP NYTaWY1ZW0GQR1XS
GCIY NV7pTHBjT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MoP4TWM2OD1{NT6yPFc4KM7:TR?= M4DxRXNCVkeHUh?=
NUGC-3 Ml7BS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NFftTWJKSzVyPUK1MlM{OTZizszN NFP3cFNUSU6JRWK=
LNCaP-Clone-FGC NGT0eI5Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MlLDTWM2OD1{NT6zOFk5KM7:TR?= M{P2XXNCVkeHUh?=
PA-1 MXnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NXLjclRmUUN3ME2yOU43QDN|IN88US=> NHnzUnBUSU6JRWK=
OE33 MlzQS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXHJR|UxRTJ3Lk[4OVMh|ryP M4PWV3NCVkeHUh?=
HT-29 NX;XcWM4T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NFj4UYdKSzVyPUK1Mlc5PzlizszN MojaV2FPT0WU
RCM-1 MXfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M{Gx[mlEPTB;MkWuPVY2PiEQvF2= M2PMTXNCVkeHUh?=
NCI-H1522 NVnMbG5GT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MlTPTWM2OD1{NT65PVEyKM7:TR?= NWe1S5IyW0GQR1XS
LB373-MEL-D Mni1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MX7JR|UxRTJ4LkG3NFgh|ryP M3zlR3NCVkeHUh?=
NCI-H1436 MkLpS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NEnqfWVKSzVyPUK2MlI4OzhizszN NUnpN|NIW0GQR1XS
COLO-824 MYTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3XqUmlEPTB;Mk[uOFAxOiEQvF2= NUnzVok1W0GQR1XS
CP50-MEL-B M2XTW2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVTlPYRzUUN3ME2yOk42Pjd3IN88US=> M2nlcnNCVkeHUh?=
EGI-1 NEHVbWdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MWrJR|UxRTJ5LkK2PVgh|ryP NELXSWZUSU6JRWK=
LAN-6 MoLiS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MoHwTWM2OD1{Nz6zOVk5KM7:TR?= NWXJRZNQW0GQR1XS
GAK NIHx[mpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MlzCTWM2OD1{Nz60NlI2KM7:TR?= MXTTRW5ITVJ?
MDA-MB-175-VII M2nKc2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFLZbodKSzVyPUK3MlUxPTNizszN NYnOVmx6W0GQR1XS
JVM-2 NXnNZYJUT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M37kZ2lEPTB;MkeuOlYzOiEQvF2= MlrrV2FPT0WU
UACC-257 NIfaNm1Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MX7JR|UxRTJ5Lk[4O|gh|ryP NFfVeYJUSU6JRWK=
SW1783 NVzQbYVnT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M{DwXGlEPTB;MkeuO|c5OyEQvF2= M3fJe3NCVkeHUh?=
HT-3 NIX5TZlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1\OZWlEPTB;MkiuNlE3PiEQvF2= NHLlempUSU6JRWK=
RL NH6wU4pIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3fGTGlEPTB;MkiuOVkyOSEQvF2= MW\TRW5ITVJ?
TK10 NH3lZ5FIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M{DE[mlEPTB;MkiuOlU{PSEQvF2= Ml3UV2FPT0WU
HT55 NXXm[Y1lT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NWmzVoZCUUN3ME2yPU4xPTZ3IN88US=> M3X1bnNCVkeHUh?=
BT-20 NF;sPJdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2G0VWlEPTB;MkmuNFU5PiEQvF2= M4S4[HNCVkeHUh?=
TGBC24TKB NWnmcpNoT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MmP4TWM2OD1{OT6xPFMyKM7:TR?= MXzTRW5ITVJ?
HSC-2 M1\XUmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M4r1NWlEPTB;MkmuN|AxOSEQvF2= NInifYpUSU6JRWK=
COLO-205 M3WydWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MV;JR|UxRTJ7LkO5OFQh|ryP MVLTRW5ITVJ?
GT3TKB NGfBVG5Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MWDJR|UxRTJ7Lk[4O|ch|ryP NYDDcYZkW0GQR1XS
T47D M{PV[Wdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFzMflZKSzVyPUK5Mlg2QDJizszN MUDTRW5ITVJ?
OMC-1 MXLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MkG0TWM2OD1|MD6xPVE6KM7:TR?= MXjTRW5ITVJ?
PFSK-1 NV3EOVVFT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Ml;YTWM2OD1|MT6yOlU6KM7:TR?= MUjTRW5ITVJ?
JEG-3 NEHIVJBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXTScJVoUUN3ME2zNU4{PTZzIN88US=> M{TqfHNCVkeHUh?=
RD MYTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M{DCZmlEPTB;M{GuOVEyPSEQvF2= NVnhN2V7W0GQR1XS
NCI-H64 NWHBVo9sT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MUDJR|UxRTN{LkSxO|kh|ryP MXrTRW5ITVJ?
DMS-114 M2nxWmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1qzZ2lEPTB;M{KuOFE5OiEQvF2= Mlu5V2FPT0WU
NCI-H661 NVHn[VdrT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M{P6S2lEPTB;M{KuOlUxOSEQvF2= M{D1ZXNCVkeHUh?=
CAL-148 NEPQbFdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NWnUbJd1UUN3ME2zNk43PjZ3IN88US=> NFK0N5dUSU6JRWK=
HuP-T3 M2C1T2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MkXxTWM2OD1|Mj65N|M5KM7:TR?= NGj3U5pUSU6JRWK=
ES4 NXzWV|V3T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NEjBOJhKSzVyPUOzMlE4ODVizszN NYiyelJ2W0GQR1XS
Ca9-22 MmP6S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NIroVFFKSzVyPUOzMlQ3ODFizszN M17OOnNCVkeHUh?=
LP-1 MXrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWrqVmMxUUN3ME2zOE4xQSEQvF2= NHHQWYlUSU6JRWK=
AU565 NYD4d3NXT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NGrBZ|ZKSzVyPUO0MlUxPjlizszN MmC2V2FPT0WU
MDA-MB-453 NVXneldDT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NFzt[ZRKSzVyPUO1MlE1OjZizszN MXzTRW5ITVJ?
SNU-C1 NGrw[nVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYPJR|UxRTN3LkG3NUDPxE1? NWX2TFViW0GQR1XS
SW13 NHzrUY9Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXj6Ond5UUN3ME2zOU41ODd4IN88US=> M1rGZnNCVkeHUh?=
NCI-H2122 NX32R4JRT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MYjJR|UxRTN3LkW1PVgh|ryP NHLib|VUSU6JRWK=
NCI-H1395 M4nDeGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M{ezWmlEPTB;M{WuOlY6OSEQvF2= MoDEV2FPT0WU
J-RT3-T3-5 MYfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MV\JR|UxRTN4LkCyPVQh|ryP MWnTRW5ITVJ?
COR-L279 MkTXS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4PzUGlEPTB;M{[uNVk5OSEQvF2= Ml7iV2FPT0WU
ATN-1 NH\RSXFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MlHpTWM2OD1|Nj64OVE3KM7:TR?= NWSyVnBRW0GQR1XS
HCC2998 MX7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NGDlcZlKSzVyPUO2Mlg2PzFizszN M{jyXHNCVkeHUh?=
UM-UC-3 MlyzS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NWrwVVFGUUN3ME2zOk45Pjd3IN88US=> MmrpV2FPT0WU
DV-90 M3PCXWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXzJR|UxRTN4LkmwOlch|ryP NYr0cGpEW0GQR1XS
ES8 NVe3OoU6T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MUXJR|UxRTN4LkmzNVgh|ryP NUjNPHo{W0GQR1XS
YAPC M3\2S2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NYfEfXNIUUN3ME2zOk46PjV2IN88US=> NFW2SW5USU6JRWK=
HCC1599 NWD1N|ZlT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3LG[2lEPTB;M{euNVYyQCEQvF2= MmewV2FPT0WU
UMC-11 NUnjWHJGT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M2\D[GlEPTB;M{euNlUxOyEQvF2= MmfUV2FPT0WU
THP-1 NVrVcVE{T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MmjzTWM2OD1|Nz6yPFY5KM7:TR?= NIDvfWJUSU6JRWK=
OCUB-M NFnrOJFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NEHBWlJKSzVyPUO3MlI5QTFizszN NGPUd4xUSU6JRWK=
NCI-H1693 NW\ud3l2T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MnnkTWM2OD1|Nz6zNlM{KM7:TR?= M1XEbHNCVkeHUh?=
NCI-H345 MUDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NUP0OGx7UUN3ME2zO{4{PjJ2IN88US=> NFvJT5dUSU6JRWK=
COLO-800 NIjKVnpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NEfGV4VKSzVyPUO3MlU3PyEQvF2= NIK5cHdUSU6JRWK=
COLO-320-HSR M3zt[Gdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NYO4bosxUUN3ME2zPE4{PDl{IN88US=> M4nNRXNCVkeHUh?=
NCI-H596 NGXUUVRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1jJS2lEPTB;M{iuOFQ5QSEQvF2= NVznO2RqW0GQR1XS
NCI-H187 NYrwVo14T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3LNO2lEPTB;M{iuO|M1PCEQvF2= MXLTRW5ITVJ?
ECC4 MoTVS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXTVPWNSUUN3ME2zPU4xQDF2IN88US=> MWLTRW5ITVJ?
KP-4 NWnmfWdWT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MULJR|UxRTN7LkGyN|Uh|ryP MXzTRW5ITVJ?
NCI-H2227 M2jJS2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mmf0TWM2OD1|OT6zPVQ6KM7:TR?= MoWyV2FPT0WU
22RV1 NHnTc2RIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MkXTTWM2OD1|OT64N|k5KM7:TR?= NWDUbmxwW0GQR1XS
SAS NUKyPXZLT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MmXKTWM2OD12MD6wPVYh|ryP NEPBSmdUSU6JRWK=
PANC-10-05 MWfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MWfJR|UxRTRyLkG4Nlgh|ryP M3m3RnNCVkeHUh?=
MDA-MB-468 MUPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NIL0XmxKSzVyPUSwMlU{PDZizszN MW\TRW5ITVJ?
RPMI-7951 MVnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVHJR|UxRTRzLkKwO|Uh|ryP NIrIR5hUSU6JRWK=
BB49-HNC NYrZU2NST3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M37vWWlEPTB;NEGuNlE4QSEQvF2= NE[xboxUSU6JRWK=
23132-87 M{fm[2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1PnO2lEPTB;NEGuNlc{OiEQvF2= NXi3RpBMW0GQR1XS
HCC1569 NF;XbVNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mmm5TWM2OD12MT6yPFg2KM7:TR?= MknXV2FPT0WU
CP66-MEL MnLCS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3jMSWlEPTB;NEKuNVg5PiEQvF2= MV\TRW5ITVJ?
K-562 MX\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NXHhZmc4UUN3ME20Nk4zQDV2IN88US=> MlrIV2FPT0WU
OCI-AML2 MkD3S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVrJR|UxRTR{Lki5O|Qh|ryP Ml\DV2FPT0WU
SW756 Mmf4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVTJR|UxRTR|LkCzPFgh|ryP M4LJfnNCVkeHUh?=
M14 NX23Z2sxT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NWr4UHI3UUN3ME20N{4{OTh7IN88US=> NXe2RWlXW0GQR1XS
NCI-H1623 NHTyRmNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NIPMcolKSzVyPUSzMlQ3PCEQvF2= NF3FPWZUSU6JRWK=
KNS-81-FD NWj4O3JsT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NEfub4RKSzVyPUSzMlc3OiEQvF2= M4rsWXNCVkeHUh?=
TGBC1TKB NWTG[nJDT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MVzJR|UxRTR|LkmwPVkh|ryP NYrqcGJDW0GQR1XS
RERF-LC-MS Ml\xS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVLJR|UxRTR|LkmyPFEh|ryP NYm3T5RrW0GQR1XS
PC-3 NIXlOohIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NIDMOnBKSzVyPUS0MlQ{PDdizszN NYnVS21vW0GQR1XS
HuP-T4 NGW5[WlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MojJTWM2OD12ND60OFc1KM7:TR?= NHP5bHpUSU6JRWK=
NCI-H510A MV7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1rhTGlEPTB;NESuO|A5KM7:TR?= NHHGOFRUSU6JRWK=
LB2518-MEL NWTReIc{T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MmP1TWM2OD12ND63PVM4KM7:TR?= M1jn[HNCVkeHUh?=
TE-5 NH\lcmlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NYrWd5p4UUN3ME20OU46PThzIN88US=> M2r3UHNCVkeHUh?=
EW-24 NYezUphCT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHH2U2lKSzVyPUS2MlE4OjNizszN Ml3pV2FPT0WU
LB647-SCLC M{DPPWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M4PxbWlEPTB;NE[uNlE2PSEQvF2= MUfTRW5ITVJ?
NCI-H524 NH2yd5VIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MkTKTWM2OD12Nj63NVg2KM7:TR?= M1P1THNCVkeHUh?=
EW-11 NI\v[nNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NFfD[4NKSzVyPUS2Mlk{PzNizszN MmrJV2FPT0WU
KALS-1 MnzCS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NFHWU2tKSzVyPUS3MlIxPjZizszN NG\GRnFUSU6JRWK=
BT-474 MXnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHW4c2VKSzVyPUS3MlQzOTZizszN NIToNZZUSU6JRWK=
SK-N-AS MUXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1\3[WlEPTB;NEeuPFg5KM7:TR?= M3;rZXNCVkeHUh?=
HCC2157 M36wdGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MoToTWM2OD12OD6wO|Yh|ryP MVXTRW5ITVJ?
HCC1954 NFTQXZFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NIfxVYJKSzVyPUS4MlE4ODRizszN NHjRVJVUSU6JRWK=
FADU MY\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MUjJR|UxRTR6LkOwN|Eh|ryP MUXTRW5ITVJ?
S-117 MU\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MoTYTWM2OD12OD6zPFE2KM7:TR?= NV23XHlPW0GQR1XS
LS-1034 NFeyPFFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MkHRTWM2OD12OD63NFY4KM7:TR?= MkfvV2FPT0WU
CAMA-1 NULZZYVQT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MlnKTWM2OD12OT61OVQ{KM7:TR?= MYjTRW5ITVJ?
LU-134-A MWfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHvDZmRKSzVyPUS5MlgxQDVizszN MmToV2FPT0WU
NCI-H1838 NXzqc48{T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NWDZSIYyUUN3ME20PU45ODh5IN88US=> NVnBPFY3W0GQR1XS

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
pSrc / Src / pPlcg / Plcg / pStat5 / Stat5 ; 

PubMed: 22781593     


In (B) inhibition of Src, Plcg and Stat5 was seen in MLNAF cell lines treated with Ponatinib; ZNF112 expresses ZMYM2–FGFR1, CEP2A and CEP5A cells express CEP110–FGFR1 and BBC2 expresses BCR–FGFR1.

pFGFR1(pY20) / FGFR1; 

PubMed: 22781593     


Western blot analysis, using various antibodies specific for downstream targets of FGFR1, demonstrates that Ponatinib inhibits the activation of Plcg and Src as a result of inhibiting the FGFR1 fusion kinase in human KG1 cells. 

cleaved caspase-3 / cleaved caspase-7 / BAX / Bcl-2; 

PubMed: 30959969     


Western blot analysis of cleaved caspase-3, cleaved caspase-7, Bax, Bcl-2, and β-Tubulin (control) in treated cells.

p-Rb(S807/811) / Rb / CDK4 / CDK6 / Cyclin D1 / CDK2 / Cyclin E1; 

PubMed: 30959969     


Western blot analysis of Rb/p-Rb, CDK4, CDK6, Cyclin D1, CDK2, Cyclin E1, and β-Tubulin (control) in the SK-Hep-1 and SNU-423 cells treated with ponatinib. 

RAD51; 

PubMed: 30959969     


The effect of ponatinib on the concentration of Rad51 in SK-Hep-1 and SNU-423 cells.

p-MEK / MEK / p-ERK / ERK; 

PubMed: 30959969     


Effect of ponatinib on the phosphorylation of Mek and Erk. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was used as a control.

c-Jun / p-JNK(T183/Y185) / JNK; 

PubMed: 30959969     


Effect of ponatinib on the phosphorylation of JNK and c-Jun. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was used as a control.

p-p38 MAPK / p38 MAPK; 

PubMed: 30959969     


Effect of ponatinib on the phosphorylation of p38. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was used as a control.

p-JAK2 / JAK2 / p-STAT3 / STAT3; 

PubMed: 30959969     


Effect of ponatinib on the phosphorylation of Jak2, and Stat3. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was used as a control.

PI3K p85 / p-PI3K / p-PDK1 / PDK1 / p-mTOR / mTOR; 

PubMed: 30959969     


Effects of ponatinib on the protein concentration and phosphorylation of PI 3-kinase, PDK1, Akt, and mTOR in HCC cells. 

p-PDGFRα / PDGFRα; 

PubMed: 24472312     


Ponatinib inhibited phosphorylation of PDGFRα in a concentration-dependent manner. EOL-1 and BaF3-WT or -T674I FIP1L1-PDGFRα cells were exposed to escalating concentrations of ponatinib for 24 h.

PARP / Caspase-3 / Active Caspase-3; 

PubMed: 24472312     


The concentration- (for 24 h) and time-dependent (1 nM for EOL-1, 300 nM for BaF3-WT and -T674I FIP1L1-PDGFRα cells) cleavage of PARP and caspase-3 triggered by ponatinib was analyzed by immunoblotting.

AIF / Cyto C ; 

PubMed: 24472312     


Ponatinib elicited release of AIF and cytochrome c into the cytosol. Cells were treated with 1 nM ponatinib for the indicated durations and the cytosolic fraction was extracted with digitonin buffer. Levels of AIF and Cytochrome c (Cyto c) were detected by immunoblotting.

Mcl-1 / Survivin / Bcl-Xl / XIAP / Bax / Bcl-2 / Bim; 

PubMed: 24472312     


Immunoblotting of apoptosis-related proteins in CEL cells after treatment for 24 h.

β-catenin ; 

PubMed: 24472312     


Ponatinib concentration-dependently lowered β-catenin. EOL-1 cells were incubated with ponatinib for 24 h, and cytoplasmic and nuclear extracts were determined by immunoblotting.

β-catenin; 

PubMed: 24472312     


Analysis of β-catenin localization. EOL-1 cells were pretreated with 1 nM ponatinib for 24 h, immunofluorescence analysis was performed with anti-β-catenin. Nuclei were stained with 4,6-diamidino-2-phenylindole (DAPI). 

ABL; 

PubMed: 19878872     


In vitro [γ-32P]-ATP autophosphorylation of full-length (A) ABL or (B) ABLT315I kinase treated with AP24534, imatinib, nilotinib, or dasatinib. After incubation of tyrosine-dephosphorylated enzyme with the indicated inhibitor in the presence of [γ-32P]-ATP and separation by SDS-PAGE, signal intensity was measured by autoradiography.

22781593 30959969 24472312 19878872
Growth inhibition assay
Cell viability; 

PubMed: 30174789     


(A) Effect of Ponatinib on basal viability of HUVECs. The cells were treated with Ponatinib at indicated concentrations or 0.1% DMSO (vehicle control) for 24 h.

30174789
In vivo In a mouse xenograft model of Ba/F3 cells expressing native Bcr-Abl, AP24534 (2.5 mg/kg and 5 mg/kg) prolongs mice median survival. In the xenograft model of Ba/F3 Bcr-AblT315I, AP24534 (10 mg/kg–50 mg/kg) significantly suppresses tumor growth. AP24534 (30 mg/kg) decreases the phosphorylated Bcr-Abl and phosphorylated CrkL levels in the tumors. [2]

Protocol

Kinase Assay:[1]
+ Expand

Peptide substrate phosphorylation assays with GST-Abl kinase domains:

The effect of AP24534 (0-320 nM) on GST-Abl kinase activity is assessed by using a synthetic peptide substrate (Abltide: EAIYAAPFAKKK). Assays are carried out at 30 °C for 15 min in 25 μL reaction mixture: 8 mM MOPS (pH 7), 0.2 mM EDTA, 50 μM Abltide, 30 mM MgCl2, 10 mM β-glycerol phosphate, 1 mM EGTA, 0.002% Brij-35, 0.4 mM DTT, 0.2 mg/mL BSA, 0.4 mM sodium orthovanadate, 10 nM WT or mutant GST-Abl kinase, and 100 µM ATP/γ-32[P]ATP (5000 cpm/pmol). Reactions are terminated by transferring a portion of the reaction mixture onto a p81 phosphocellulose filter and immersing in 0.75% phosphoric acid. Filters are washed 3 times in 0.75% phosphoric acid, rinsed in acetone, and air dried; phosphate incorporation is determined by scintillation counting. All results are corrected for background binding to the filters, as determined by omitting peptide substrate from the kinase reaction. Time course experiments to establish the linear range of enzymatic activity precedes kinase assays.
Cell Research:[2]
+ Expand
  • Cell lines: Ba/F3 cells expressing Bcr-Abl or a range of single mutations in the kinase domain of Bcr-Abl
  • Concentrations: 0-625 nM
  • Incubation Time: 72 hours
  • Method: Ba/F3 cell lines are distributed in 96-well plates (4 × 103 cells/well) and incubated with AP24534 for 72 hours. Proliferation is measured using a methanethiosulfonate (MTS)-based viability assay (CellTiter96 Aqueous One Solution). All values are normalized to the control wells with no drug. IC50 values are reported as the mean of three independent experiments performed in quadruplicat
    (Only for Reference)
Animal Research:[1]
+ Expand
  • Animal Models: Mouse xenograft models of Ba/F3 cells expressing Bcr-Abl or Bcr-AblT315I
  • Formulation: Dissolved in 25 mM citrate buffer, pH 2.75
  • Dosages: 2.5 mg/kg and 5 mg/kg for Ba/F3 Bcr-Abl; 2.5 mg/kg–50 mg/kg for Ba/F3 Bcr-AblT315I
  • Administration: Oral gavage once daily
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 30 mg/mL (56.33 mM)
Water Insoluble
Ethanol Insoluble
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
1% CMC Na
For best results, use promptly after mixing.
30mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 532.56
Formula

C29H27F3N6O

CAS No. 943319-70-8
Storage powder
in solvent
Synonyms N/A

Bio Calculators

Molarity Calculator

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Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

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Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

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Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

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Clinical Trial Information

NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT03838692 Not yet recruiting Medullary Thyroid Cancer Antonio Fojo|Millennium Pharmaceuticals Inc.|Columbia University April 2019 Phase 2
NCT03838692 Not yet recruiting Medullary Thyroid Cancer Antonio Fojo|Millennium Pharmaceuticals Inc.|Columbia University April 2019 Phase 2
NCT03878524 Not yet recruiting Breast Cancer|Prostate Cancer|Pancreatic Cancer|Acute Myelogenous Leukemia OHSU Knight Cancer Institute|Oregon Health and Science University|Prospect Creek Foundation March 14 2019 Phase 1
NCT03878524 Not yet recruiting Breast Cancer|Prostate Cancer|Pancreatic Cancer|Acute Myelogenous Leukemia OHSU Knight Cancer Institute|Oregon Health and Science University|Prospect Creek Foundation March 14 2019 Phase 1
NCT03807479 Recruiting Leukemia Myeloid Chronic-Phase GWT-TUD GmbH|Incyte Biosciences International Sàrl December 11 2018 Phase 2
NCT03807479 Recruiting Leukemia Myeloid Chronic-Phase GWT-TUD GmbH|Incyte Biosciences International Sàrl December 11 2018 Phase 2

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

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Frequently Asked Questions

  • Question 1:

    Is the pre-solubilized inhibitor suitable for in vivo whole animal studies? Whether the solvent contains any non-DMSO stabilizer?

  • Answer:

    Our 10mM Ponatinib in DMSO solution is in pure DMSO and there is not other stabilizer added. You can use it for animal study. The vehicle we suggest for Ponatinib is: 30% PEG400/0.5% Tween80/5% Propylene glycol. It is a milky suspension used for oral gavage.

Bcr-Abl Signaling Pathway Map

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID