3'-Hydroxypterostilbene

For research use only.

Catalog No.S3940 Synonyms: 3'-HPT

3'-Hydroxypterostilbene Chemical Structure

Molecular Weight(MW): 272.30

3'-Hydroxypterostilbene (3'-HPT) is one of the active constituents of Sphaerophysa salsula and Pterocarpus marsupium which may be useful in treating different types of haematological malignancies. 3'-Hydroxypterostilbene, a natural pterostilbene analogue, effectively inhibits the growth of human colon cancer cells (IC50s of 9.0, 40.2, and 70.9 µM for COLO 205, HCT-116, and HT-29 cells, respectively) by inducing apoptosis and autophagy. 3'-Hydroxypterostilbene inhibits the PI3K/Akt/mTOR/p70S6K, and p38MAPK pathways and activates the ERK1/2, JNK1/2 MAPK pathways.

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Biological Activity

Description 3'-Hydroxypterostilbene (3'-HPT) is one of the active constituents of Sphaerophysa salsula and Pterocarpus marsupium which may be useful in treating different types of haematological malignancies. 3'-Hydroxypterostilbene, a natural pterostilbene analogue, effectively inhibits the growth of human colon cancer cells (IC50s of 9.0, 40.2, and 70.9 µM for COLO 205, HCT-116, and HT-29 cells, respectively) by inducing apoptosis and autophagy. 3'-Hydroxypterostilbene inhibits the PI3K/Akt/mTOR/p70S6K, and p38MAPK pathways and activates the ERK1/2, JNK1/2 MAPK pathways.
In vitro

3'-Hydroxypterostilbene decreases cell growth in cultured human colon cancer cells (COLO 205, HCT-116, and HT-29) in a dose-dependent manner, with IC50 values of 9.0, 40.2, and 70.9 µM, respectively. 3'-Hydroxypterostilbene effectively inhibits the growth of human colon cancer cells by inducing apoptosis and autophagy. Treatment with HPSB causes reduction of mitochondrial membrane potential and induction of caspase-3 and caspage-9, which is associated with the degradation of DFF-45 and PARP, precedes the onset of apoptosis. It significantly down-regulates phosphatidylinositol 3-kinase (PI3K)/Akt and mitogen-activated protein kinases (MAPKs) signalings including decreased the phosphorylation of mammalian target of rapamycin (mTOR)[1].

In vivo 3'-Hydroxypterostilbene by i.p. injection has anti-tumor efficacy gainst colon cancer through the inhibition of inflammation, metastasis, and angiogenesis as well as through the induction of apoptosis[1].

Protocol

Cell Research:

[1]

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  • Cell lines: COLO 205 cells
  • Concentrations: 5-100 µM
  • Incubation Time: 24 h
  • Method:

    For sub-G1 cell population analysis, COLO 205 cells (2×105 cells/mL) are cultured in 12 well and treatment with various concentrations of pterostilbene or 3′-hydroxypterostilbene for 24 h. The cells are then harvested, washed with PBS, resuspended in 200 µL of PBS, and fixed in 800 µL of iced 100% ethanol at -20°C. After being left to stand overnight, the cell pellets are collected by centrifugation, resuspended in 1 mL of hypotonic buffer (0.5% Triton X-100 in PBS and 0.5 µg/mL RNase) with PI (50 µg/mL) and incubated at 37°C in the dark for 30 min. Fluorescence emitted from the PI-DNA complex is quantitated after excitation of the fluorescent dye.


    (Only for Reference)
Animal Research:

[1]

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  • Animal Models: nude mice bearing COLO 205 tumor xenografts
  • Dosages: 10 mg/kg
  • Administration: i.p.
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 54 mg/mL (198.31 mM)
Ethanol 54 mg/mL (198.31 mM)
Water Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 272.30
Formula

C16H16O4

CAS No. 475231-21-1
Storage powder
in solvent
Synonyms 3'-HPT
Smiles COC1=CC(=CC(=C1)C=CC2=CC(=C(C=C2)O)O)OC

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Clinical Trial Information

NCT Number Recruitment interventions Conditions Sponsor/Collaborators Start Date Phases
NCT01199250 Not yet recruiting Other: Laboratory Biomarker Analysis Lynch Syndrome|Recurrent Uterine Corpus Carcinoma|Stage I Uterine Corpus Cancer|Stage II Uterine Corpus Cancer|Stage III Uterine Corpus Cancer|Stage IV Uterine Corpus Cancer Gynecologic Oncology Group|National Cancer Institute (NCI) January 2100 --
NCT03643887 Not yet recruiting Drug: FMT Capsule DE|Drug: Placebo oral capsule Clostridium Difficile Infection University of Wisconsin Madison September 1 2022 Phase 2
NCT03737617 Suspended Drug: Cuvitru 20 % Injectable Solution Bronchiectasis|Immunoglobulin Subclass Deficiency University of Edinburgh|NHS Lothian August 5 2022 Phase 4
NCT03912350 Not yet recruiting Drug: Balovaptan Autism Spectrum Disorder Hoffmann-La Roche June 26 2022 Phase 1
NCT04259190 Withdrawn Behavioral: interoceptive exposure Anxiety Sensitivity Gina Boullion|University of Mississippi Oxford June 1 2022 Not Applicable

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID