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3'-Hydroxypterostilbene S6 Kinase inhibitor

Name: 3'-Hydroxypterostilbene
Brand: Selleck Chemicals
SKU: S3940
Availability: InStock
Rating: 5 (3 reviews)

Cat.No.S3940

3'-Hydroxypterostilbene (3'-HPT) is one of the active constituents of Sphaerophysa salsula and Pterocarpus marsupium which may be useful in treating different types of haematological malignancies. This compound, a natural pterostilbene analogue, effectively inhibits the growth of human colon cancer cells (IC50s of 9.0, 40.2, and 70.9 µM for COLO 205, HCT-116, and HT-29 cells, respectively) by inducing apoptosis and autophagy. It inhibits the PI3K/Akt/mTOR/p70S6K, and p38MAPK pathways and activates the ERK1/2, JNK1/2 MAPK pathways.

Chemical Structure

Molecular Weight: 272.30

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Quality Control

Batch: S394001 DMSO]54 mg/mL]false]Ethanol]54 mg/mL]false]Water]Insoluble]false Purity: 99.55%

Cited in Nature Medicine for its top-tier quality
COA
HPLC
SDS
Datasheet

99.55

Related Targets	ERK p38 MAPK Raf JNK MEK Ras KRas MAP4K TAK1 Mixed Lineage Kinase
Other S6 Kinase Inhibitors	PF-4708671 BI-D1870 LY2584702 LY2584702 Tosylate LJH685 LJI308 S6K-18 BRD7389 L-Norvaline Gingerenone A

Solubility

In vitro
Batch:

DMSO : 54 mg/mL (198.31 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)

Ethanol : 54 mg/mL

Water : Insoluble

Molarity Calculator

Mass	Concentration	Volume	Molecular Weight
Mass value Mass unit	Concentration value Concentration unit	Volume value Volume unit	Molecular weight (g/mol)

Dilution Calculator Molecular Weight Calculator

In vivo batch selection In vivo Batch:
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

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Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Chemical Information, Storage & Stability

Molecular Weight	272.30	Formula	C₁₆H₁₆O₄	Storage (From the date of receipt)	3 years-20°Cpowder
CAS No.	475231-21-1	Download SDF		Storage of Stock Solutions	1 year-80°Cin solvent 1 month-20°Cin solvent
Synonyms	3'-HPT			Smiles	COC1=CC(=CC(=C1)C=CC2=CC(=C(C=C2)O)O)OC

Mechanism of Action

Targets/IC₅₀/Ki	p70S6K p38MAPK
In vitro	3'-Hydroxypterostilbene decreases cell growth in cultured human colon cancer cells (COLO 205, HCT-116, and HT-29) in a dose-dependent manner, with IC50 values of 9.0, 40.2, and 70.9 µM, respectively. This compound effectively inhibits the growth of human colon cancer cells by inducing apoptosis and autophagy. Treatment with this compound causes reduction of mitochondrial membrane potential and induction of caspase-3 and caspage-9, which is associated with the degradation of DFF-45 and PARP, precedes the onset of apoptosis. It significantly down-regulates phosphatidylinositol 3-kinase (PI3K)/Akt and mitogen-activated protein kinases (MAPKs) signalings including decreased the phosphorylation of mammalian target of rapamycin (mTOR).
In vivo	3'-Hydroxypterostilbene by i.p. injection has anti-tumor efficacy gainst colon cancer through the inhibition of inflammation, metastasis, and angiogenesis as well as through the induction of apoptosis.
References	[1] https://pubmed.ncbi.nlm.nih.gov/25389774/

Clinical Trial Information

(data from https://clinicaltrials.gov, updated on 2024-05-22)

NCT Number	Recruitment	Conditions	Sponsor/Collaborators	Start Date	Phases
NCT01199250	Not yet recruiting	Lynch Syndrome\|Recurrent Uterine Corpus Carcinoma\|Stage I Uterine Corpus Cancer\|Stage II Uterine Corpus Cancer\|Stage III Uterine Corpus Cancer\|Stage IV Uterine Corpus Cancer	Gynecologic Oncology Group\|National Cancer Institute (NCI)\|GOG Foundation	January 2100	--
NCT05454306	Suspended	Clavicle Fracture	University of Minnesota	January 9 2028	Not Applicable
NCT05118594	Not yet recruiting	Major Depressive Disorder\|Dysthymic Disorder\|Specific Phobia\|Social Anxiety Disorder\|Panic Disorder\|Agoraphobia\|Generalized Anxiety Disorder	National Council of Scientific and Technical Research Argentina	February 2026	Not Applicable
NCT06077526	Not yet recruiting	Chronic Pain	Bridgewater College	January 13 2026	Not Applicable

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