Home Primary Antibodies ZO1 tight junction protein Antibody [A24G22]

research use only

ZO1 tight junction protein Antibody [A24G22]

Catalog No.: F2530

    Application: Reactivity:

    Usage Information

    Dilution
    1:500
    1:100
    1:60
    Application
    IHC, IF, FCM
    Reactivity
    Rat, Human, Mouse
    Source
    Rabbit Monoclonal Antibody
    Storage Buffer
    PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3
    Storage (from the date of receipt)
    -20°C (avoid freeze-thaw cycles), 2 years

    Datasheet & SDS

    Biological Description

    Specificity
    ZO1 tight junction protein Antibody [A24G22] detects endogenous levels of total ZO1 tight junction protein.
    Clone
    A24G22
    Synonym(s)
    ZO1; TJP1; Tight junction protein ZO-1; Tight junction protein 1; Zona occludens protein 1; Zonula occludens protein 1
    Background
    ZO-1 (Zonula Occludens-1) is a peripheral membrane phosphoprotein belonging to the membrane-associated guanylate kinase (MAGUK) family and serves as a critical scaffolding molecule in tight junctions. ZO-1 contains three PDZ domains that facilitate protein-protein interactions, an SH3 domain that binds various signaling proteins, and a guanylate kinase (GuK) domain connected by flexible hinge regions; its unique C-terminal region directly binds to the actin cytoskeleton, anchoring the tight junction complex to maintain cellular integrity. ZO-1 organizes transmembrane tight junction proteins such as occludin and claudins, linking them to cytosolic partners like ZO-2 and ZO-3, thus playing an essential role in assembling and maintaining the tight junction barrier that controls paracellular permeability. ZO-1 also acts as a signaling hub regulating cell growth, migration, angiogenesis, and inflammation and helps maintain epithelial cell polarity by preserving the boundary between apical and basolateral membrane domains. ZO-1 is vital for tissue homeostasis in barriers like intestinal epithelium and the blood-brain barrier, with its downregulation associated with diseases such as inflammatory bowel disease (IBD) and cancer progression, where compromised tight junctions contribute to pathological permeability and tumorigenesis.
    References
    • https://pubmed.ncbi.nlm.nih.gov/16436508/
    • https://pubmed.ncbi.nlm.nih.gov/19605556/

    Tech Support

    Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

    Handling Instructions

    Tel: +1-832-582-8158 Ext:3
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