Home Primary Antibodies VSV-G tag Antibody [N12B16]

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VSV-G tag Antibody [N12B16]

Catalog No.: F2031

    Application: Reactivity:

    Usage Information

    Dilution
    1:1000
    1:5000
    1:2000
    1:500
    Application
    WB, IHC, IF, FCM
    Reactivity
    Source
    Rabbit Monoclonal Antibody
    Storage Buffer
    PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3
    Storage (from the date of receipt)
    -20°C (avoid freeze-thaw cycles), 2 years
    Observed MW
    100 kDa
    *Why do the predicted and actual molecular weights differ?
    The following reasons may explain differences between the predicted and actual protein molecular weight.

    Datasheet & SDS

    Biological Description

    Specificity
    VSV-G tag Antibody [N12B16] detects recombinant proteins containing the VSV-G epitope tag.
    Clone
    N12B16
    Synonym(s)
    VSV epitope tag; VSV tag; VSV-G epitope tag; VSVG; Vesicular stomatitis virus glycoprotein tag; vsvg tag
    Background
    The VSV-G tag is a short, highly immunogenic epitope (sequence: QMRTRRHLHV) derived from the membrane-proximal C-terminal region of vesicular stomatitis virus glycoprotein (VSV-G), a class III viral fusion protein belonging to the rhabdovirus glycoprotein family. The native VSV-G ectodomain folds into three conserved domains: a fusion domain (FD) with membrane-interacting loops (residues ~118-136), pleckstrin homology domain (PHD), and trimerization domain (TrD), connected by flexible hinge regions (R1-R5) that undergo pH-induced refolding; the tag corresponds to a linear, solvent-exposed antigenic site in the post-fusion conformation recognized by neutralizing antibodies like PIV. When fused to recombinant proteins, the small VSV-G tag (11 amino acids) minimally perturbs native folding and enables sensitive detection via commercial monoclonal antibodies in immunoblotting, immunoprecipitation, immunofluorescence, and flow cytometry without affecting subcellular localization or interactions. Native VSV-G mediates broad receptor binding (e.g., LDL-R, CR2/CR3 domains via basic residues H8, K47, R354), clathrin-mediated endocytosis, and low-pH-triggered fusion through irreversible trimer rearrangement, underpinning its use in pseudotyping lentiviral/retroviral vectors for stable, high-titer gene delivery across diverse cell types with no disease relevance as a tag.
    References
    • https://pubmed.ncbi.nlm.nih.gov/20921141/
    • https://pubmed.ncbi.nlm.nih.gov/18345480/

    Tech Support

    Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

    Handling Instructions

    Tel: +1-832-582-8158 Ext:3
    If you have any other enquiries, please leave a message.

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