Thyroglobulin Antibody [K18H12] | Primary Antibodies

Application: Reactivity: Mouse, Rat, Human

Usage Information

Dilution
WB1:10000 - 1:50000 IHC1:250 - 1:500 IF1:50 - 1:100 FCM1:70

Application
WB, IHC, IF, FCM

Reactivity
Mouse, Rat, Human

Source
Rabbit Monoclonal Antibody

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW Observed MW
305 kDa 294-300 kDa
^*Why do the predicted and actual molecular weights differ? The following reasons may explain differences between the predicted and actual protein molecular weight.

Positive Control	Rat thyroid tissue; Human thyroid tissue; Mouse thyroid tissue; ICR thyroid tissue; Human thyroid gland papillary carcinoma tissue; Human thyroid gland follicular carcinoma tissue; Human thyroid carcinoma epithelial cells
Negative Control

Datasheet & SDS

Biological Description

Specificity
Thyroglobulin Antibody [K18H12] detects endogenous levels of total Thyroglobulin protein.

Clone
K18H12

Synonym(s)
Thyroglobulin; Tg; TG

Background
Thyroglobulin (Tg) is a large glycoprotein that acts as the precursor for thyroid hormones T3 and T4. It is primarily synthesized and secreted by thyroid follicular cells into the colloid lumen, where it undergoes iodination. Tg is a dimer composed of three main regions: region I contains 10-11 type-1 repeats; region II features three type-2 repeats forming arrowhead-like motifs stabilized by inter-domain disulfide bonds; and region III includes type-3 repeats, all leading up to a C-terminal ChEL domain near the dimer core. Key hormonogenic tyrosines, such as sites B and D, are strategically positioned for iodination, and conserved cysteine motifs like CWCV in type-1 domains help maintain structural integrity. Tg serves as the scaffold for thyroid peroxidase-mediated iodination and the coupling of iodotyrosines to produce T3/T4 precursors. These precursors are endocytosed and proteolyzed in lysosomes, releasing thyroid hormones into circulation. Tg intrinsically regulates thyroid hormone biosynthesis by suppressing the transcription of essential genes, including Tg itself, TPO, NIS (Slc5a5), and DUOX2, through apical interactions. This negative feedback mechanism counteracts TSH stimulation, helping to maintain follicular heterogeneity and optimize hormone output. The cycle of Tg accumulation and resorption is crucial for this regulation. Disruptions, such as Tg mutations leading to ER stress and impaired secretion, are linked to diseases like congenital hypothyroidism.

Background

Thyroglobulin (Tg) is a large glycoprotein that acts as the precursor for thyroid hormones T3 and T4. It is primarily synthesized and secreted by thyroid follicular cells into the colloid lumen, where it undergoes iodination. Tg is a dimer composed of three main regions: region I contains 10-11 type-1 repeats; region II features three type-2 repeats forming arrowhead-like motifs stabilized by inter-domain disulfide bonds; and region III includes type-3 repeats, all leading up to a C-terminal ChEL domain near the dimer core. Key hormonogenic tyrosines, such as sites B and D, are strategically positioned for iodination, and conserved cysteine motifs like CWCV in type-1 domains help maintain structural integrity. Tg serves as the scaffold for thyroid peroxidase-mediated iodination and the coupling of iodotyrosines to produce T3/T4 precursors. These precursors are endocytosed and proteolyzed in lysosomes, releasing thyroid hormones into circulation. Tg intrinsically regulates thyroid hormone biosynthesis by suppressing the transcription of essential genes, including Tg itself, TPO, NIS (Slc5a5), and DUOX2, through apical interactions. This negative feedback mechanism counteracts TSH stimulation, helping to maintain follicular heterogeneity and optimize hormone output. The cycle of Tg accumulation and resorption is crucial for this regulation. Disruptions, such as Tg mutations leading to ER stress and impaired secretion, are linked to diseases like congenital hypothyroidism.

References
https://pubmed.ncbi.nlm.nih.gov/26595189/ https://pubmed.ncbi.nlm.nih.gov/24251883/

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%