SMURF 2 Antibody [A20L4] | Primary Antibodies

Application: Reactivity: Human

Lane 1: HeLa, Lane 2: HeLa (KO SMURF2), Lane 3: U-2 OS

Usage Information

Dilution
WB1:500 - 1:1000

Application
WB

Reactivity
Human

Source
Rabbit Monoclonal Antibody

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW Observed MW
86 kDa 86 kDa
^*Why do the predicted and actual molecular weights differ? The following reasons may explain differences between the predicted and actual protein molecular weight.

Positive Control	HeLa cells; U-2 OS cells; SH-SY-5Y cells
Negative Control

Datasheet & SDS

Biological Description

Specificity
SMURF 2 Antibody [A20L4] detects endogenous levels of total SMURF 2 protein.

Clone
A20L4

Synonym(s)
E3 ubiquitin-protein ligase SMURF2; hSMURF2; HECT-type E3 ubiquitin transferase SMURF2; SMAD ubiquitination regulatory factor 2; SMAD-specific E3 ubiquitin-protein ligase 2; SMURF2

Background
Smurf2 (Smad ubiquitin regulatory factor 2) is a member of the NEDD4 family of HECT-type E3 ubiquitin ligases. It plays a pivotal role in negatively regulating TGF-β/BMP signaling pathways and helping maintain cellular homeostasis through targeted protein degradation. Smurf2 features an N-terminal C2 domain, which mediates membrane association and autoinhibition by binding to its own HECT domain. The central region contains three WW domains that recognize PPXY motifs on substrates or adaptor proteins, such as Smad7. Its C-terminal HECT catalytic domain possesses an active-site cysteine (Cys716) crucial for ubiquitin transfer, and this domain remains autoinhibited until activated by adaptor binding or phosphorylation. Smurf2 partners with Smad7 to target TGF-β type I receptors and receptor-regulated Smads (Smad1, Smad2, and Smad3) for ubiquitination and subsequent proteasomal degradation, effectively suppressing TGF-β/BMP signaling. Smurf2 also regulates the stability of other proteins, including RUNX2, ID2, EZH2, RNF20, and Topo IIα, influencing processes such as osteogenesis, cellular senescence, genomic stability, and cell cycle arrest. The role of Smurf2 in cancer is context-dependent: it can act as a tumor suppressor (through RNF20-mediated H2Bub1 regulation) or promote tumorigenesis (by enhancing migration in colorectal cancer).

Background

Smurf2 (Smad ubiquitin regulatory factor 2) is a member of the NEDD4 family of HECT-type E3 ubiquitin ligases. It plays a pivotal role in negatively regulating TGF-β/BMP signaling pathways and helping maintain cellular homeostasis through targeted protein degradation. Smurf2 features an N-terminal C2 domain, which mediates membrane association and autoinhibition by binding to its own HECT domain. The central region contains three WW domains that recognize PPXY motifs on substrates or adaptor proteins, such as Smad7. Its C-terminal HECT catalytic domain possesses an active-site cysteine (Cys716) crucial for ubiquitin transfer, and this domain remains autoinhibited until activated by adaptor binding or phosphorylation. Smurf2 partners with Smad7 to target TGF-β type I receptors and receptor-regulated Smads (Smad1, Smad2, and Smad3) for ubiquitination and subsequent proteasomal degradation, effectively suppressing TGF-β/BMP signaling. Smurf2 also regulates the stability of other proteins, including RUNX2, ID2, EZH2, RNF20, and Topo IIα, influencing processes such as osteogenesis, cellular senescence, genomic stability, and cell cycle arrest. The role of Smurf2 in cancer is context-dependent: it can act as a tumor suppressor (through RNF20-mediated H2Bub1 regulation) or promote tumorigenesis (by enhancing migration in colorectal cancer).

References
https://pubmed.ncbi.nlm.nih.gov/32733916/ https://pubmed.ncbi.nlm.nih.gov/11016919/

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%