Pyk2 Antibody [L19B9] | Primary Antibodies

Application: Reactivity: Human, Mouse

Lane 1: Jurkat, Lane 2: Ramos, Lane 3: Raji

Usage Information

Dilution
WB1:1000 IP1:50

Application
WB, IP

Reactivity
Human, Mouse

Source
Mouse Monoclonal Antibody

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW
116 kDa

Positive Control	Jurkat cells; Ramos cells; Raji cells
Negative Control

Datasheet & SDS

Biological Description

Specificity
Pyk2 Antibody [L19B9] detects endogenous levels of total Pyk2 protein.

Clone
L19B9

Synonym(s)
Protein-tyrosine kinase 2-beta; Calcium-dependent tyrosine kinase (CADTK); CAK-beta; CAKB; Focal adhesion kinase 2 (FADK 2); Proline-rich tyrosine kinase 2; Related adhesion focal tyrosine kinase (RAFTK); PTK2B; FAK2; PYK2; RAFTK

Background
Pyk2 (proline-rich tyrosine kinase 2) is a non-receptor tyrosine kinase closely related to FAK, highly expressed in hematopoietic cells and neurons. It comprises an N-terminal FERM domain that regulates autoinhibition and oligomerization, a central bilobal kinase domain with key autophosphorylation sites at Tyr402 (activation loop) and Tyr579/580 (regulatory tyrosines), proline-rich regions (PR1/PR2) for SH3-domain interactions, and a C-terminal focal adhesion targeting (FAT) four-helix bundle domain that binds paxillin LD motifs to localize at cell adhesions. Activation is triggered by Ca2+ influx or integrin/GPCR engagement, which relieves FERM autoinhibition and enables Tyr402 autophosphorylation, leading to Src recruitment and subsequent phosphorylation of Tyr579/580. This initiates downstream signaling through pathways such as p130Cas-Crk-DOCK180-Rac1/CDC42 for cytoskeletal remodeling and migration, Grb2-SOS-Ras-ERK1/2 for gene expression and proliferation, and PI3K-AKT for survival, while FAT-paxillin scaffolding amplifies focal adhesion turnover essential for chemotaxis in macrophages/neutrophils and synaptic plasticity in neurons. Negative regulation occurs via calpain-mediated cleavage or PP2 phosphatases. Dysregulated Pyk2 activity drives osteoclast hyperactivity in arthritis by enhancing migration and invasion, neuronal excitotoxicity in epilepsy and stroke through Ca2+-dependent overactivation, and cancer metastasis (breast, prostate, glioblastoma) by promoting invadopodia formation and anoikis resistance.

Background

Pyk2 (proline-rich tyrosine kinase 2) is a non-receptor tyrosine kinase closely related to FAK, highly expressed in hematopoietic cells and neurons. It comprises an N-terminal FERM domain that regulates autoinhibition and oligomerization, a central bilobal kinase domain with key autophosphorylation sites at Tyr402 (activation loop) and Tyr579/580 (regulatory tyrosines), proline-rich regions (PR1/PR2) for SH3-domain interactions, and a C-terminal focal adhesion targeting (FAT) four-helix bundle domain that binds paxillin LD motifs to localize at cell adhesions. Activation is triggered by Ca2+ influx or integrin/GPCR engagement, which relieves FERM autoinhibition and enables Tyr402 autophosphorylation, leading to Src recruitment and subsequent phosphorylation of Tyr579/580. This initiates downstream signaling through pathways such as p130Cas-Crk-DOCK180-Rac1/CDC42 for cytoskeletal remodeling and migration, Grb2-SOS-Ras-ERK1/2 for gene expression and proliferation, and PI3K-AKT for survival, while FAT-paxillin scaffolding amplifies focal adhesion turnover essential for chemotaxis in macrophages/neutrophils and synaptic plasticity in neurons. Negative regulation occurs via calpain-mediated cleavage or PP2 phosphatases. Dysregulated Pyk2 activity drives osteoclast hyperactivity in arthritis by enhancing migration and invasion, neuronal excitotoxicity in epilepsy and stroke through Ca2+-dependent overactivation, and cancer metastasis (breast, prostate, glioblastoma) by promoting invadopodia formation and anoikis resistance.

References
https://pubmed.ncbi.nlm.nih.gov/21482413/ https://pubmed.ncbi.nlm.nih.gov/25174335/

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%