Home Primary Antibodies PDK1 Antibody [E12L3]

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PDK1 Antibody [E12L3]

Cat.No.: F3393

    Application: Reactivity:
    • F3393-wb
      Lane 1: Hela, Lane 2: Hela (KO PDK1), Lane 3: C6, Lane 4: L-929

    Usage Information

    Dilution
    1:2000
    1:1000
    1:1000
    1:600
    Application
    WB, IP, IF, FCM
    Reactivity
    Mouse, Rat, Human
    Source
    Rabbit Monoclonal Antibody
    Storage Buffer
    PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3
    Storage (from the date of receipt)
    -20°C (avoid freeze-thaw cycles), 2 years
    Predicted MW Observed MW
    49 kDa 47 kDa, 45 kDa
    *Why do the predicted and actual molecular weights differ?
    The following reasons may explain differences between the predicted and actual protein molecular weight.
    Positive Control Mouse brain tissue; Mouse kidney tissue; Mouse spleen tissue; Mouse heart tissue; Rat brain tissue; Rat kidney tissue; Rat heart tissue; HAP1 cells; HeLa cells; Jurkat cells; L-929 cells; RAW 264.7 cells; NIH/3T3 cells; F9 cells; bEnd.3 cells; C6 cells; PC-12 cells
    Negative Control

    Datasheet & SDS

    Biological Description

    Specificity
    PDK1 Antibody [E12L3] detects endogenous levels of total PDK1 protein.
    Clone
    E12L3
    Synonym(s)
    PDHK1; PDK1; Pyruvate dehydrogenase kinase isoform 1; PDH kinase 1
    Background
    Phosphoinositide-dependent kinase 1 (PDK1) is a serine/threonine kinase of the AGC family encoded by PDPK1, acting as a master regulator transducing PI3K signals from growth factors like insulin to activate over 20 AGC kinases, including Akt, S6K, RSK, SGK, and PKC isoforms, crucial for cell survival, proliferation, metabolism, and migration. PDK1 features an N-terminal kinase domain with constitutively autophosphorylated Ser241 in the activation loop and a PIF-pocket (Gln150, Thr148, Ile119) for hydrophobic motif docking on substrates, as well as a C-terminal pleckstrin homology (PH) domain (Arg472, Arg474, Lys465) that binds PIP3/PI(3,4)P2, recruiting PDK1 to the plasma membrane. There, phosphoinositide engagement relieves cytosolic autoinhibition, promotes side-to-side dimerization, and enables high-affinity phosphorylation of activation loops (e.g., Akt Thr308, S6K Thr389) through coordinated alignment of ATP and substrate in the active site. This amplifies PI3K/Akt/mTOR signaling for protein synthesis (via 4E-BP1/S6K), glucose uptake (via AS160), anti-apoptosis (via Bad/FoxO), and cytoskeletal remodeling (via PKC/Rho), while regulating PDH phosphorylation for Warburg-like metabolic reprogramming in cancer. PDK1 is essential for development, as shown by embryonic lethality in knockout mice, and its overexpression or hyperactivation in breast, lung, and prostate cancers is linked to poor prognosis and metastasis through unchecked PI3K signaling.
    References
    • https://pubmed.ncbi.nlm.nih.gov/35387990/
    • https://pubmed.ncbi.nlm.nih.gov/37792325/

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