NMDA Receptor 1 (GluN1) Antibody [D10D12]

Catalog No.: F0486

For research use only.

    Home Primary Antibodies NMDA Receptor 1 (GluN1) Antibody [D10D12]

    Application: Reactivity:

    Usage Information

    Dilution
    1:1000
    1:50
    Application
    WB, IP
    Reactivity
    Human, Mouse, Rat
    Source
    Rabbit Monoclonal Antibody
    Storage Buffer
    PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3
    Storage (from the date of receipt)
    -20°C (avoid freeze-thaw cycles), 2 years
    Predicted MW
    120 kDa

    Datasheet & SDS

    Biological Description

    Specificity
    NMDA Receptor 1 (GluN1) Antibody [D10D12] detects endogenous levels of total NMDA Receptor 1 (GluN1) protein.
    Clone
    D10D12
    Synonym(s)
    amparnmdar
    Background
    NMDA Receptor 1 (GluN1) (N-Methyl-D-aspartate receptor subunit 1) is the obligatory glycine/D-serine-binding subunit present in all functional NMDA receptors, making it essential for receptor assembly and function in virtually all central neurons. Structurally, GluN1 consists of an extracellular amino-terminal domain (ATD) that regulates allosteric modulation, a bilobed agonist-binding domain (ABD) that binds glycine or D-serine, a transmembrane domain forming part of the ion channel pore, and an intracellular carboxyl-terminal domain (CTD) that interacts with scaffolding and signaling proteins such as PSD-95, calmodulin, and NF-L; alternative splicing of exons 5, 21, and 22 generates eight isoforms with distinct regulatory properties. Widely expressed across the CNS, GluN1 pairs with GluN2 (A–D) and sometimes GluN3 subunits to form heterotetrameric receptors whose properties depend on subunit composition. Functionally, GluN1-containing receptors act as ligand-gated ion channels that require both glutamate (binding to GluN2) and glycine/D-serine (binding to GluN1) for activation, and their voltage-dependent Mg²⁺ block and high Ca²⁺ permeability make them central to synaptic transmission, long-term potentiation, excitatory postsynaptic current duration, and activity-dependent synaptic plasticity underlying learning and memory.
    References
    • https://pubmed.ncbi.nlm.nih.gov/30037851/

    Tech Support

    Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

    Handling Instructions

    Tel: +1-832-582-8158 Ext:3
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